Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye

Bioactive toxins from stinging jellyfish

“…Cyanea nozakii can be observed in warm and temperate waters, especially along the coasts of China (Li et al, 2011). The venom from this jellyfish was lethal to the grass carp, Ctenopharyngodon idellus.…”

Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris

“…The nematocyst, elliptic and rod-like cell, is primarily in the surface of tentacles and functions as assault and defense arm. It is composed of a complex mixture with many bioactivities, such as enzymatic activities, hemolysis, lethality, cardiovascular toxicity and neurotoxicity and so on [17][18][19][20][21]. Therefore, in present study, an antioxidant protein named as SmP90 was successfully isolated and identified from the nematocyst of jellyfish S. meleagris by ammonium sulfate precipitation and superdex 75 with the antioxidant activity characterized for the first time.…”

“…The tentacles were excised manually from living specimens as soon as possible after capture and were then immediately frozen at −80 • C for subsequent use. The nematocysts were prepared according to the method we described before [19]. Briefly, after autolysis overnight at 4 • C, the tentacles were stirred for 10 min and filtered through a plankton net (∼280 m) to remove most of the tentacle debris.…”

Application of nanoLC–MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris

“…The tentacles were excised manually from living specimens as soon as possible after capture and were then immediately frozen at −80 • C for subsequent use. The extraction method of nematocyst proteins was the same as we described before [3]. Briefly, the nematocyst was sonicated 30 min for 10 s intervals in cold extraction buffer (4 • C, 20 mM Tris-HCl, pH 7.8, 0.15 M NaCl, 1 mM EDTA, 5 g/mL Pepstatin A and 0.5 mM PMSF).…”

“…The nematocyst, which is predominantly in the surface of jellyfish tentacles, contains many complex and diversiform biomolecules with plenty of activities, such as hemolytic activity, lethal activity, cytotoxic activity, neurotoxic activity, insecticidal activity, antioxidant activity, cardiovascular activity, enzyme activity and other potential activities [1][2][3][4][5][6][7][8][9][10]. As a result, increasing attention had been attracted to investigate the jellyfish nematocyst proteins, including bioactivity analysis, isolation, and identification of bioactive molecules.…”