Two-Step Sensing, Colorimetric and Ratiometric Fluorescent Probe for Rapid Detection of Bisulfite in Aqueous Solutions and in Living Cells

Abstract

Bisulfite and sulfite (HSO3–/SO32–) are not only widely used toxic chemicals but also active anions with important biological functions. Hence, the development of new detection methods for HSO3–/SO32– is important for environmental security and human health. In this paper, we report a symmetrical hemicyanine for the detection of HSO3–/SO32–, SHC, which is constructed with p-diphthalaldehyde with trimethylbenzoindolium via condensation. The red fluorescent probe can fast respond to HSO3–/SO32– (<30 s) to give cyan fluorescence, and its sensing process is twice nucleophilic additions, which were observed from fluorescence response, initial ratiometric change, and subsequent turn-on increment; especially in a low-concentration level, the ratiometric fluorescence measurement can eliminate environmental interference. This probe can achieve a quantitative detection of HSO3–/SO32– in a wide concentration range. Furthermore, the probe SHC is a mitochondria-specific probe for ratiometric fluorescent detection of HSO3–/SO32– in living cells.

A ratiometric and colorimetric probe for detecting Hg2+ based on naphthalimide–rhodamine and its staining function in cell imaging

“…Fluorescent probes have become powerful tools for the detection of heavy metal ions due to their low cost, high selectivity, and low concentration. [1][2][3][4][5] Among heavy metal ions, Hg 2+ is one of the most troublesome ions because it easily goes through the biolm, and then causes serious damage to the central nervous and endocrine systems. [6][7][8][9][10] In view of this, the synthesis of probes to identify Hg 2+ has attracted sustained attention.…”

A 1-Hydroxy-2,4-Diformylnaphthalene-Based Fluorescent Probe and Its Detection of Sulfites/Bisulfite

“…1 H NMR (600 MHz, CD 3 OD): δ 10.21 (s, 1H), 10.04 (s, 1H), 9.20-9.22 (d, J = 12 Hz, 1H), 9.00-9.07 (d, J = 42 Hz, 1H), 8.00-8.02 (d, J = 12 Hz, 1H), 6.92-7.92 (m, 4H), 6.68-6.59 (d, J = 61H), 5.81-5.83 (d, J = 12, 1H), 5.33 (s, 1H), 3.90 (s, 1H), 2.75 (s, 3H), 1.35 (s, 3H), 1.35 (s, 3H). 13…”

“…The following are available online, Figure S1: HRMS spectrum of L title, Figure S2: 1 H NMR of probe L, Figure S3: 13 Funding: This research received no external funding.…”

A ratiometric fluorescent probe for the rapid and specific detection of HSO3− in water samples

“…Ratiometric fluorescent probes can greatly reduce the adverse effects of these interference factors and improve the sensitivity, specificity and quantitative detection range because they use the ratio of fluorescence intensities at two different emission wavelengths as the detection parameter. [36][37][38][39][40][41][42] Although some fluorescent probes based on the ratiometric principle have been reported, [43][44][45][46][47][48][49][50][51][52] these still have some shortcomings such as complex structure and long response time (Table S1).…”