Two TIP‐like genes encoding aquaporins are expressed in sunflower guard cells

Sarda, Xavier; Tousch, Didier; Ferrare, Karine; LeGrand, Edmund K.; Dupuis, J.; Casse-Delbart, Francine; Lamaze, Thierry

doi:10.1046/j.1365-313x.1997.12051103.x

Cited by 135 publications

(76 citation statements)

References 34 publications

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“…The theoretical possibility that hydraulic resistance of the guard cell membrane is temporarily increased (e.g. by regulation of aquaporins; Sarda et al, 1997;Huang et al, 2002), which would prevent quick equilibration, appears counterproductive because this would impede turgor loss of guard cells in a situation that demands stomatal closure.…”

Section: Discussionmentioning

confidence: 99%

Localization of Mechanisms Involved in Hydropassive and Hydroactive Stomatal Responses of Sambucus nigra to Dry Air

Kaiser

Legner

2006

Plant Physiology

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The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading to active closure are not yet known. This study attempts to evaluate the importance of a single pore's transpiration on its own response and that of adjacent pores. Selected stomata on attached intact leaves of Sambucus nigra were sealed with mineral oil and the response to a reduction of humidity was continuously observed in situ. Blocking a pore's transpiration had no appreciable effect on hydropassive opening and subsequent stomatal closure. If the adjacent stomata were additionally sealed, the closing response was reduced, but not the hydropassive opening. On the other hand, sealing the entire leaf surface, except a small area including the observed stomata, also reduced stomatal closure. These results indicate that strictly local processes triggered by a pore's own transpiration are not required to induce stomatal closure. To describe the effect of one pore's transpiration on the hydropassive and hydroactive responses of neighboring stomata, a simple spatial model was constructed. It suggests that 90% of the closing effect covers an area of approximately 0.5 mm 2 , whereas the effect on hydropassive opening affects an area of approximately 1 mm 2 . This divergence may suggest mechanisms other than or in addition to those involving changes of local leaf water potential.

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Section: Discussionmentioning

confidence: 99%

Localization of Mechanisms Involved in Hydropassive and Hydroactive Stomatal Responses of Sambucus nigra to Dry Air

Kaiser

Legner

2006

Plant Physiology

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“…High expression levels of particular PIP and TIP aquaporins have been associated with: (i) high cell expansion rates Kaldenhoff et al 1995;Chaumont et al 1998;Weig & Eisenbarth 2000) (ii) cell differentiation (Kaldenhoff et al 1995); (iii) cells that exhibit large changes in volume, e.g. guard cells and pulvinar motor cells (Kaldenhoff et al 1995;Sarda et al 1997;Fleuratlessard et al 1997) (iv) high rates of water flow through cells (Barrieu, Chaumont & Chrispeels 1998;Kirch et al 2000;Otto & Kaldenhoff 2000;Frangne et al 2001) (v) the formation of a large central vacuole . For three TIP isoforms (γ α δ) there is an association with different types of vacuole (Jauh et al 1998;Jauh, Phillips & Rogers 1999).…”

Section: Patterns Of Mip Locationmentioning

confidence: 99%

“…Some members of the TIP-family also showed changed expression patterns when plants were exposed to water stress: rTIP in rice (Liu, Umeda & Uchimiya 1994), SunTIP7 in sunflower (Sarda et al 1997), and Bob-TIP26 in cauliflower (Barrieu et al 1999) were all expressed more. Conversely SunTIP18 in sunflower (Sarda et al 1999) and Cp-TIP in the resurrection plant (Mariaux et al 1998) and Mc-MIP F in Mesembryanthemum crystallinum (Kirch et al 2000) were down-regulated, when the plants lacked water.…”

Section: Effect Of Water Stress On the Expression Of Mip Genesmentioning

confidence: 99%

Plant aquaporins: multifunctional water and solute channels with expanding roles

Tyerman¹,

Niemietz²,

Bramley³

2002

Plant Cell & Environment

561

360

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There is strong evidence that aquaporins are central components in plant water relations. Plant species possess more aquaporin genes than species from other kingdoms. According to sequence similarities, four major groups have been identified, which can be further divided into subgroups that may correspond to localization and transport selectivity. They may be involved in compatible solute distribution, gas-transfer (CO 2 , NH 3 ) as well as in micronutrient uptake (boric acid). Recent advances in determining the structure of some aquaporins gives further details on the mechanism of selectivity. Gating behaviour of aquaporins is poorly understood but evidence is mounting that phosphorylation, pH, pCa and osmotic gradients can affect water channel activity. Aquaporins are enriched in zones of fast cell division and expansion, or in areas where water flow or solute flux density would be expected to be high. This includes biotrophic interfaces between plants and parasites, between plants and symbiotic bacteria or fungi, and between germinating pollen and stigma. On a cellular level aquaporin clusters have been identified in some membranes. There is also a possibility that aquaporins in the endoplasmic reticulum may function in symplasmic transport if water can flow from cell to cell via the desmotubules in plasmodesmata. Functional characterization of aquaporins in the native membrane has raised doubt about the conclusiveness of expression patterns alone and need to be conducted in parallel. The challenge will be to elucidate gating on a molecular level and cellular level and to tie those findings into plant water relations on a macroscopic scale where various flow pathways need to be considered.

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“…In the latter material, expression of most PIP genes was higher during the day than at night (Heinen et al, 2014). In sunflower (Helianthus annuus) guard cells, expression of vacuolar aquaporin SunTIP7 also showed diurnal variations, with an expression peak in phase with stomatal closure (Sarda et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…However, it has remained unclear whether the accompanying water flow across the guard cell plasma membrane and tonoplast can simply be mediated by the lipid phase of membranes or whether aquaporins, channel proteins that facilitate transmembrane water transport, are involved. In fact, information on aquaporin expression in guard cells has remained scarce (Sarda et al, 1997;Sun et al, 2001;Fraysse et al, 2005;Shope and Mott, 2006;Yang et al, 2006;Heinen et al, 2014). Plasma membrane intrinsic proteins (PIPs) represent the most abundant aquaporins in the plant plasma membrane (Santoni et al, 2003), and transcriptome analysis indicated expression of 8 and 12 PIP isoforms in stomata of Arabidopsis thaliana and stomatal complexes of maize (Zea mays), respectively (Leonhardt et al, 2004;Heinen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation

et al. 2015

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International audienceStomatal movements in response to environmental stimuli critically control the plant water status. Although these movements are governed by osmotically driven changes in guard cell volume, the role of membrane water channels (aquaporins) has remained hypothetical. Assays in epidermal peels showed that knockout Arabidopsis thaliana plants lacking the Plasma membrane Intrinsic Protein 2;1 (PIP2;1) aquaporin have a defect in stomatal closure, specifically in response to abscisic acid (ABA). ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells, which were both abrogated in pip2;1 plants. Open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6), a protein kinase involved in guard cell ABA signaling, was able to phosphorylate a cytosolic PIP2;1 peptide at Ser-121. OST1 enhanced PIP2;1 water transport activity when coexpressed in Xenopus laevis oocytes. Upon expression in pip2;1 plants, a phosphomimetic form (Ser121Asp) but not a phosphodeficient form (Ser121Ala) of PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-dependent activation and was able to restore ABA-dependent stomatal closure in pip2;1. This work supports a model whereby ABA-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121

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Two TIP‐like genes encoding aquaporins are expressed in sunflower guard cells

Cited by 135 publications

References 34 publications

Localization of Mechanisms Involved in Hydropassive and Hydroactive Stomatal Responses of Sambucus nigra to Dry Air

Localization of Mechanisms Involved in Hydropassive and Hydroactive Stomatal Responses of Sambucus nigra to Dry Air

Plant aquaporins: multifunctional water and solute channels with expanding roles

Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation

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