Two-trace two-dimensional correlation spectra (2T2D-COS) analysis using FTIR spectra to monitor the immune response by COVID-19

Abstract

There is a growing trend in using saliva for SARS-CoV-2 detection with reasonable accuracy. We have studied the responses of IgA, IgG, and IgM in human saliva by directly comparing disease with control analyzing two-trace two-dimensional correlation spectra (2T2D-COS) employing Fourier transform infrared (FTIR) spectra. The findings show the decrease of IgG and IgM salivary antibodies in the 50, 60, 65, and 75-years samples. Among the various COVID-19 populations studied, the female 30 years group reveal defence mechanisms exhibited by IgM and IgA. Lipids and fatty acids decrease, resulting in lipid oxidation due to the SARS-CoV-2 in the samples studied. Study shows salivary thiocyanate plays defence against SARS-CoV-2 in male population in 25 and 35 age groups. The receiver operation characteristics statistical method shows a sensitivity of 98% and specificity 94% for the samples studied. The measure of accuracy computed as F1 score and G score has a high value which supports the validation of our study. The 2T2D-COS analysis has the potential to monitor the progression of immunoglobulin’s response function of COVID-19 with good accuracy, which could help in diagnosing clinical trials.