Two translesion synthesis DNA polymerase genes, AtPOLH and AtREV1, are involved in development and UV light resistance in Arabidopsis

Santiago, María Jesús; Alejandre-Durán, Encarna; Muñoz-Serrano, A.; Ruiz-Rubio, Manuel

doi:10.1016/j.jplph.2007.11.012

Cited by 9 publications

(9 citation statements)

References 36 publications

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“…On the other hand, the residual lesions detectable after long repair incubation support the concept that some parts of the genome might be more refractory to repair and therefore plants might have also evolved mechanisms allowing them to tolerate CPDs present at lower levels in their genome (Britt ). In agreement with such an assumption, it was shown that plant translesion DNA polymerases were able to replicate CPD containing DNA, thus enhancing the UV‐tolerance in Arabidopsis (Santiago et al ).…”

Section: Discussionmentioning

confidence: 69%

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

Manova

Georgieva

Borisov

et al. 2016

Physiologia Plantarum

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Barley stress response to ultraviolet radiation (UV) has been intensively studied at both the physiological and morphological level. However, the ability of barley genome to repair UV-induced lesions at the DNA level is far less characterized. In this study, we have investigated the relative contribution of light-dependent and dark DNA repair pathways for the efficient elimination of cyclobutane pyrimidine dimers (CPDs) from the genomic DNA of barley leaf seedlings. The transcriptional activity of barley CPD photolyase gene in respect to the light-growth conditions and UV-C irradiation of the plants has also been analyzed. Our results show that CPDs induced in the primary barley leaf at frequencies potentially damaging DNA at the single-gene level are removed efficiently and exclusively by photorepair pathway, whereas dark repair is hardly detectable, even at higher CPD frequency. A decrease of initially induced CPDs under dark is observed but only after prolonged incubation, suggesting the activation of light-independent DNA damage repair and/or tolerance mechanisms. The green barley seedlings possess greater capacity for CPD photorepair than the etiolated ones, with efficiency of CPD removal dependent on the intensity and quality of recovering light. The higher repair rate of CPDs measured in the green leaves correlates with the higher transcriptional activity of barley CPD photolyase gene. Visible light and UV-C radiation affect differentially the expression of CPD photolyase gene particularly in the etiolated leaves. We propose that the CPD repair potential of barley young seedlings may influence their response to UV-stress.

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Section: Discussionmentioning

confidence: 69%

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

Manova

Georgieva

Borisov

et al. 2016

Physiologia Plantarum

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“…It plays a major role in shoot apical meristem (SAM) development in rice [32]. DNA polymerase beta is involved in DNA synthesis during DNA replication, increasing resistance against ultraviolet light [33,34]. The UMP-CMP kinase is involved in the pyrimidine biosynthetic pathway and is elevated during seedling development and fruit ontogeny [35].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Phosphoproteins in Somatic Embryogenesis Acquisition during Oil Palm Tissue Culture

Aroonluk

Roytrakul

Jantasuriyarat

2019

Plants

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Somatic embryogenesis during oil palm tissue culture is a long process. The identification of the proteins that control this process may help to shorten the time of oil palm tissue culture. We collected embryogenic callus and somatic embryos at the globular, torpedo, and cotyledon maturation stages, as well as from plantlets, for total protein extraction. An enrichment column was used to enrich the phosphoproteins, which were subjected to tryptic enzyme digestion. Each sample was analyzed with nano-liquid chromatography–tandem mass spectrometry (nano LC-MS/MS). A total of 460 phosphoproteins were identified and analyzed. The functional characterization of phosphoproteins were observed as highest in the metabolic process, protein/nucleotide/ion binding, and membrane component. The different phosphoproteins are involved in the control of vegetative growth, cellular differentiation, cell morphogenesis, and signaling roles in plants. The Quantitative Real-Time Reverse Transcription-PCR technique (qPCR) was successfully used to verify the expression of genes, and the results were consistent with the level of protein expression from nano-LC-MS/MS. The E3 ubiquitin-protein ligase and sister chromatid cohesion PDS5 were specifically expressed only in the somatic embryo and plantlet, and these could be used as protein biomarkers to determine the oil palm somatic embryo maturation stage. This study sheds light on the protein phosphorylation mechanism that regulates somatic embryogenesis transition during oil palm tissue culture.

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“…1 µl of the reverse transcriptase (RT) reaction product served as template in a 32 cycle PCR with Taq DNA polymerase (Qiagen, Hilden, Germany) using Rad54-RT-LP and Rad54-RP primers, which bind within the 19th and 21st exon, respectively. Amplification of APT1 (adenine phosphoribosyl transferase 1) transcripts with primers AtAPT1-F (5′-CCTCCTATTGCGTTGGCTATTG-3′) and AtAPT1-R (5′-TCTCCTTTCCCTTAAGCTCTGG-3′) was compared as internal control (Santiago et al 2008). PCR and RT-PCR products were analyzed by agarose gel electrophoresis and ethidium bromide staining.…”

Section: Total Nucleic Acid Extractionmentioning

confidence: 99%

Rad54 is not essential for any geminiviral replication mode in planta

2014

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The circular single-stranded DNA of phytopathogenic geminiviruses is propagated by three modes: complementary strand replication (CSR), rolling circle replication (RCR) and recombination-dependent replication (RDR), which need host plant factors to be carried out. In addition to necessary host polymerases, proteins of the homologous recombination repair pathway may be considered essential, since geminiviruses are particularly prone to recombination. Among several others, Rad54 was suggested to be necessary for the RCR of Mungbean yellow mosaic India virus. This enzyme is a double-stranded DNA-dependent ATPase and chromatin remodeller and was found to bind and modulate the viral replication-initiator protein in vitro and in Saccharomyces cerevisiae. In contrast to the previous report, we scrutinized the requirement of Rad54 in planta for two distinct fully infectious geminiviruses with respect to the three replication modes. Euphorbia yellow mosaic virus and Cleome leaf crumple virus were inoculated into Rad54-deficient and wildtype Arabidopsis thaliana plant lines to compare the occurrence of viral DNA forms. Replication intermediates were displayed in the time course of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization. The experiments showed that Rad54 was neither essential for CSR, RCR nor RDR, and it had no significant influence on virus titers during systemic infection.

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Two translesion synthesis DNA polymerase genes, AtPOLH and AtREV1, are involved in development and UV light resistance in Arabidopsis

Cited by 9 publications

References 36 publications

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

Efficient removal of cyclobutane pyrimidine dimers in barley: differential contribution of light‐dependent and dark DNA repair pathways

Identification and Characterization of Phosphoproteins in Somatic Embryogenesis Acquisition during Oil Palm Tissue Culture

Rad54 is not essential for any geminiviral replication mode in planta

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