Two unisexual artificial polyploid clones constructed by genome addition of common carp (Cyprinus carp) and crucian carp (Carassius auratus)

Wu, Qingping; Ye, Yuzhen; Dong, Xinhong

doi:10.1360/02yc0198

Cited by 16 publications

(18 citation statements)

References 17 publications

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“…These allotetraploid carps are able to produce their offspring by the process of gynogenesis (Wu et al 2003). We have found no difference in morphometric traits, cytogenetic traits, and PCR markers between the gynogenetic offspring of the allotetraploid carp and their female parent (Ye et al 2002;Wu et al 2003). In this study of the process of polyploidization, the red crucian carp acted as the maternal parent.…”

Section: Discussionmentioning

confidence: 54%

“…The tetraploid carp, with two sets of chromosomes from the common carp and the crucian carp, were produced as described by Wu et al (2003). In this study, the firstgeneration gynogenetic tetraploid carp (gF1) were produced using one female allotetraploid carp (Fac) and sperm (as inducer) from one of five species: Chinese blunt snout bream (Megalobrama amblycephala), red crucian carp (Carassius auratus), common carp (Cyprinus carpio), gudgeon topmouth (Pseudorasbora parva), and sharpbelly (Hemiculter leucisculus).…”

Section: Experimental Fishes and Production Of Two Generations Of Gynmentioning

confidence: 99%

“…This carp was generated by genome addition of the common carp (Cyprinus carpio) and the crucian carp (Carassius auratus). Our early studies indicated that morphometric traits, cytogenetic traits, and PCR markers of the gynogenetic offspring of the allotetraploid carp were identical to their female parent (Ye et al 2002;Wu et al 2003). To investigate the contribution of male genetic material to the artificial gynogenetic tetraploid offspring, we used microsatellite markers and the D-loop sequence of mitochondrial DNA (mtDNA) to compare the allotetraploid parental female and its offspring generated gynogenetically using sperm from five different fish species.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species

Wang

Zhou

et al. 2009

Biochem Genet

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Microsatellite markers and D-loop sequences of mtDNA from a female allotetraploid parent carp and her progenies of generations 1 and 2 induced by sperm of five distant fish species were analyzed. Eleven microsatellite markers were used to identify 48 alleles from the allotetraploid female. The same number of alleles (48) appeared in the first and second generations of the gynogenetic offspring, regardless of the source of the sperm used as an activator. The mtDNA D-loop analysis was performed on the female tetraploid parent, 25 gynogenetic offspring, and 5 sperm-donor species. Fourteen variable sites from the 1,018 bp sequences were observed in the offspring as compared to the female tetraploid parent. Results from D-loop sequence and microsatellite marker analysis showed exclusive maternal transmission, and no genetic information was derived from the father. Our study suggests that progenies of artificial tetraploid carp are genetically stable, which is important for genetic breeding of this tetraploid fish.

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Section: Discussionmentioning

confidence: 54%

Section: Experimental Fishes and Production Of Two Generations Of Gynmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species

Wang

Zhou

et al. 2009

Biochem Genet

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“…There were already some reports about the formations of tetraploids by controlling chromosomes at home and abroad [13] . But it was the first time to report on the formation of bisexual fertile tetraploids producing diploid gametes stably by means of androgenesis.…”

Section: The Ploidy Level and Fertility Of Amentioning

confidence: 99%

The formation of improved tetraploid population of red crucian carp × common carp hybrids by androgenesis

et al. 2007

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Bisexual fertile diploid androgenetic individuals (A(0)) (2n=100) were formed by androgenesis. In this way, the diploid spermatozoa from male allotetraploid hybrids (AT) (4n=200) of red crucian carp (Carassius auratus red var.) (female) x common carp (Cyprinus carpio L.) (male) were used to fertilize the UV-treated haploid eggs of goldfish (Carassius auratus), and living androgenetic diploid fish were developed. The A(0) became sexually mature at the age of 2 years, and they fertilized with each other to form their offspring (A(1)). In this study, we observed the chromosomal number, gonadal structure and appearance of A(1) fish. The results are as follows: (1) In A(1), there were 85% tetraploids (A(1)-4n), 10% triploids (A(1)-3n) and 5% diploids (A(1)-2n), suggesting that diploid A(0) could produce diploid gametes. It was concluded that the formation of diploid gametes generated from diploid A(0) was probably related to the mechanism of pre-meiotic endoreduplication. (2) Among A(1), only A(1)-4n possessed normal ovaries and testes. The mature males of A1-4n produced white semen. Under the electron microscope, the head of diploid sperm generated by A(1)-4n was bigger than that of haploid sperm generated by red crucian carp. In the testes of the A(1)-4n, there were many mature normal spermatozoa with a head bearing plasma membrane and a tail having the typical structure of "9+2" microtubules. Between the head and the tail, there were some mitochondria. The ovaries of A(1)-4n developed well and mainly contained II, III and IV-stage oocytes. The IV-stage oocytes were surrounded by inner and outer follicular cells. The micropyle was observed on the oolemma of follicular cells. There were abundant yolks and plenty of endoplasmic reticulum in the cytoplasm of IV-stage oocytes. Because A(1)-2n and A(1)-3n were distant crossing diploid hybrids and triploid hybrids respectively, they possessed abnormal gonads, and no mature semen and eggs were observed. (3) Compared with allotetraploids, the A(1)-4n fish not only had advantages such as fast growth rate and strong resistibility but also showed some new good performances such as high ratio of body width to body length, smaller heads and shorter tails. These results indicated that androgenesis could produce bisexual fertile tetraploids and improve the shape of allotetraploid hybrids as well, which will be of great significance in both the cell genetics research and fish breeding.

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“…(♀) × red crucian carp (Carassius auratus red var.) (♂) generated diploid eggs which fertilized with the haploid sperm and formed the triploid hybrids with the ability to produce the triploid eggs [5] . The triploid eggs developed into triploid offspring following activation by UV-irradiated sperm.…”

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confidence: 99%

Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

et al. 2007

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This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G(3)), which was generated from the diploid eggs produced by the second gynogenetic generation (G(2)) of red crucian carp x common carp, and determined the chromosomal numbers of G(3), G(2)xscatter scale carp and G(2)xallotetraploid hybrids of red crucian carp x common carp. The results showed that the diploid eggs of G(2) with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G(3) with 100 chromosomes. Similar to the first and second gynogenetic generations (G(1) and G(2)), G(3) was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G(2) with scatter scale carp, and crossing G(2) with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G(2) ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G(2). The establishment of the diploid gynogenesis clonal line (G(1), G(2) and G(3)) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gynogenetic progeny of allotetraploid hybrids of red crucian carp x common carp had important significances in both biological evolution and production application.

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Two unisexual artificial polyploid clones constructed by genome addition of common carp (Cyprinus carp) and crucian carp (Carassius auratus)

Cited by 16 publications

References 17 publications

Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species

Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species

The formation of improved tetraploid population of red crucian carp × common carp hybrids by androgenesis

Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

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