Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses

Hannan, P. C. T.; Bhogal, B.S.; Fish, J. P.

doi:10.1016/s0034-5288(18)32364-6

Cited by 130 publications

(83 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pigs that showed severe respiratory distress during the observation period were euthanized. All of surviving pigs were euthanized at day 14 post-challenge, and the lung lesions were examined and scored as described previously [25]. Briefly, the lung lesion was determined by divided the complete lung into seven lobes, each lobe was scored 1-5 by assessing the pneumonic area.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Xie

Zhou

et al. 2016

BMC Vet Res

View full text Add to dashboard Cite

Background Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs.ResultsThe S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1.0 × 109 CFU. Furthermore, the S-8△clpP△apxIIC mutant was able to induce a strong immune response in pigs, which included high levels of IgG1 and IgG2, stimulated gamma interferon (IFN-γ), interleukin 12 (IL-12), and interleukin 4 (IL-4) production, and conferred effective protection against the lethal challenge with A. pleuropneumoniae serovars 7 or 5a. The pigs in the S-8△clpP△apxIIC immunized groups have no lesions and reduced bacterial loads in the lung tissue after challenge.ConclusionsThe data obtained in this study suggest that the S-8△clpP△apxIIC mutant can serve as a highly immunogenic and potential live attenuated vaccine candidate against A. pleuropneumoniae infection.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Pigs that showed severe respiratory distress during the observation period were euthanized. At day 14 post-challenge, all surviving pigs were euthanized and lung lesions were examined and scored as mentioned above [25]. …”

Section: Methodsmentioning

confidence: 99%

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Xie

Zhou

et al. 2016

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…In this scheme, each pulmonary lobe is divided by a number of triangles depending on the size of the lobe (7 for each cranial and middle lobe, 19 for each diaphragmatic lobe and 8 for the accessory one) [11]. The number of triangles affected with lesions per lobe is multiplied per five and divided by the number of triangles of each lobe (those lobes entirely affected with these lesions would have a score of 5).…”

Section: Methodsmentioning

confidence: 99%

Comparison of four lung scoring systems for the assessment of the pathological outcomes derived from Actinobacillus pleuropneumoniae experimental infections

et al. 2014

View full text Add to dashboard Cite

BackgroundIn this study, four lung lesion scoring methods (Slaughterhouse Pleurisy Evaluation System [SPES], Consolidation Lung Lesion Score [LLS], Image analyses [IA] and Ratio of lung weight/body weight [LW/BW]) were compared for the assessment of the different pathological outcomes derived from an Actinobacillus pleuropneumoniae (App) experimental infection model. Moreover, pathological data was coupled with clinical (fever, inappetence and clinical score), production (average daily weigh gain [ADWG]) and diagnostic (PCR, ELISA and bacterial isolation) parameters within the four infection outcomes (peracute, acute, subclinically infected and non-infected).ResultsFrom the 61 inoculated animals, 9 were classified as peracute (presence of severe App-like clinical signs and lesions and sudden death or euthanasia shortly after inoculation), 31 as acutely affected (presence of App-like clinical signs and lesions and survival until the end of the experiment), 12 as subclinically infected (very mild or no clinical signs but App infection confirmed) and 9 as non-infected animals (lack of App-like clinical signs and lack of evidence of App infection). A significant correlation between all lung lesion scoring systems was found with the exception of SPES score versus LW/BW. SPES showed a statistically significant association with all clinical, production and diagnostic (with the exception of PCR detection of App in the tonsil) variables assessed. LLS and IA showed similar statistically significant associations as SPES, with the exception of seroconversion against App at necropsy. In contrast, LW/BW was statistically associated only with App isolation in lungs, presence of App-like lesions and ELISA OD values at necropsy.ConclusionsIn conclusion, SPES, LLS and IA are economic, fast and easy-to-perform lung scoring methods that, in combination with different clinical and diagnostic parameters, allow the characterization of different outcomes after App infection.

show abstract

“…Macroscopic lung alterations were quantified according to the Lung Lesion Score (LLS) proposed by Hannan et al [30] and specified in the European Pharmacopoeia for vaccine development [31]. Briefly, lung tissue alterations assessed by visual inspection and palpation were recorded in a schematic map of the porcine lung consisting of 74 triangles (7 triangles for cardial and apical lobes each, 8 triangles for the accessory lobe, 19 triangles for each of the diaphragmatic lobes).…”

Section: Methodsmentioning

confidence: 99%

Actinobacillus pleuropneumoniae challenge in swine: diagnostic of lung alterations by infrared thermography

Menzel

Beyerbach

Siewert³

et al. 2014

BMC Vet Res

View full text Add to dashboard Cite

BackgroundActinobacillus pleuropneumoniae (A.pp.) is the causative agent of porcine pleuropneumonia leading to high economic losses in the pig industry. Infrared thermography (IRT) of the thorax might offer a new method to select swine with lung alterations for further diagnostics.In this study 50 german landrace pigs were infected with A.pp. in an established model for respiratory tract disease, while 10 healthy pigs served as control animals. To avoid drift errors during IR measurements absolute skin temperatures and temperature differences between a thoracal and an abdominal region were assessed for its diagnostic validity.ResultsIRT findings during the course of experimental A.pp.-infection were verified by computed tomography (CT) before and on days 4 and 21 after infection. Significant correlations were found between clinical scores, CT score and lung lesion score. Ambient temperature, body temperature and abdominal surface temperature were factors influencing the skin surface temperature of the thorax. On day 4 but not on day 21 after infection the right thoracal temperature was significantly higher and the difference between a thoracal region in the height of the left 10th vertebra and an abdominal region was significantly lower in infected pigs than in control pigs. At a cut off of 28°C of right thoracal temperature the specificity of the method was 100% (CI 95%: 69-100%) and the sensitivity 66% (CI 95%: 51-79%).At a cut off of 2°C temperature difference between thoracal and abdominal region on the left body site the specificity of the method was 100% (CI 95%: 69-100%) and the sensitivity 32% (CI 95%: 19-47%) with all control pigs detected negative.Orientation for lung biopsy by IRT resulted in 100% specificity and sensitivity (CI 95%: 69-100%) of bacteriological examination of tissue samples during the acute stage of infection.ConclusionIRT might be a valuable tool for the detection of inflammatory lung alterations in pigs, especially during the acute stage of infection and if ambient temperatures are constant during individual measurements. External and internal factors interfere with this method, so that its application in the field might be restricted to a selection of pigs for further diagnostic with adequate specificity.

show abstract

Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses

Cited by 130 publications

References 10 publications

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Comparison of four lung scoring systems for the assessment of the pathological outcomes derived from Actinobacillus pleuropneumoniae experimental infections

Actinobacillus pleuropneumoniae challenge in swine: diagnostic of lung alterations by infrared thermography

Contact Info

Product

Resources

About