Type 1 collagen: Synthesis, structure and key functions in bone mineralization

Selvaraj, Vimalraj; Sekaran, Saravanan; Dhanasekaran, Anuradha; Warrier, Sudha

doi:10.1016/j.diff.2024.100757

Cited by 13 publications

(2 citation statements)

References 82 publications

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“…Collagen is a member of the fibrous protein family dominating in the extracellular matrix (ECM), which provides structural support to the ECM as well as stabilizes growth factors, and regulates cell adhesion and communication between cells and the ECM ( Das et al, 2019 ; Selvaraj et al, 2024 ). Collagen type I, expressed in nearly all ECM and connective tissues in the human body, is the most abundant type of collagen, with its most significant functional roles in the skin while collagen type III occupies about 8%–11% ( Davison-Kotler et al, 2019 ; Amirrah et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of inflammatory suppression and fibroblast infiltration in tissue remodelling by supercritical CO2 acellular dermal matrix (scADM) utilizing Sprague Dawley models

Ngan Giang,

Le,

Ngoc Chien

et al. 2024

Front. Bioeng. Biotechnol.

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Human skin-derived ECM aids cell functions but can trigger immune reactions; therefore it is addressed through decellularization. Acellular dermal matrices (ADMs), known for their regenerative properties, are used in tissue and organ regeneration. ADMs now play a key role in plastic and reconstructive surgery, enhancing aesthetics and reducing capsular contracture risk. Innovative decellularization with supercritical carbon dioxide preserves ECM quality for clinical use. The study investigated the cytotoxicity, biocompatibility, and anti-inflammatory properties of supercritical CO2 acellular dermal matrix (scADM) in vivo based on Sprague Dawley rat models. Initial experiments in vitro with fibroblast cells confirmed the non-toxic nature of scADM and demonstrated cell infiltration into scADMs after incubation. Subsequent tests in vitro revealed the ability of scADM to suppress inflammation induced by lipopolysaccharides (LPS) presenting by the reduction of pro-inflammatory cytokines TNF-α, IL-6, IL-1β, and MCP-1. In the in vivo model, histological assessment of implanted scADMs in 6 months revealed a decrease in inflammatory cells, confirmed further by the biomarkers of inflammation in immunofluorescence staining. Besides, an increase in fibroblast infiltration and collagen formation was observed in histological staining, which was supported by various biomarkers of fibroblasts. Moreover, the study demonstrated vascularization and macrophage polarization, depicting increased endothelial cell formation. Alteration of matrix metalloproteinases (MMPs) was analyzed by RT-PCR, indicating the reduction of MMP2, MMP3, and MMP9 levels over time. Simultaneously, an increase in collagen deposition of collagen I and collagen III was observed, verified in immunofluorescent staining, RT-PCR, and western blotting. Overall, the findings suggested that scADMs offer significant benefits in improving outcomes in implant-based procedures as well as soft tissue substitution.

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Section: Discussionmentioning

confidence: 99%

Assessment of inflammatory suppression and fibroblast infiltration in tissue remodelling by supercritical CO2 acellular dermal matrix (scADM) utilizing Sprague Dawley models

Ngan Giang,

Le,

Ngoc Chien

et al. 2024

Front. Bioeng. Biotechnol.

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show abstract

“…Collagen is a member of the fibrous protein family dominating in the extracellular matrix (ECM), which provides structural support to the ECM as well as stabilizes growth factors, and regulates cell adhesion and communication between cells and the ECM (Das et al, 2019;Selvaraj et al, 2024). Collagen type I, expressed in nearly all ECM and connective tissues in the human body, is the most abundant type of collagen, with its most significant functional roles in the skin while collagen type III occupies about 8%-11% (Davison-Kotler et al, 2019;Amirrah et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

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Thermoresponsive and Supramolecular Polymers: Interesting Biomaterials for Drug Delivery

Darvishi,

Ansari

2024

Biotechnology Journal

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How to use and deliver drugs to diseased and damaged areas has been one of the main concerns of pharmacologists and doctors for a long time. With the efforts of researchers, the advancement of technology, and the involvement of engineering in the health field, diverse and promising approaches have been studied and used to achieve this goal. A better understanding of biomaterials and the ability of production equipment led researchers to offer new drug delivery systems to the world. In recent decades, responsive polymers (exclusively to temperature and pH) and supramolecular polymers have received much attention due to their unique capabilities. Although this field of research still needs to be scrutinized and studied more, their recognition, examination, and use as drug delivery systems is a start for a promising future. This review study, focusing on temperature‐responsive and supramolecular biomaterials and their application as drug delivery systems, deals with their structure, properties, and role in the noninvasive and effective delivery of medicinal agents.

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Type 1 collagen: Synthesis, structure and key functions in bone mineralization

Cited by 13 publications

References 82 publications

Assessment of inflammatory suppression and fibroblast infiltration in tissue remodelling by supercritical CO2 acellular dermal matrix (scADM) utilizing Sprague Dawley models

Assessment of inflammatory suppression and fibroblast infiltration in tissue remodelling by supercritical CO2 acellular dermal matrix (scADM) utilizing Sprague Dawley models

DataSheet1.ZIP

Thermoresponsive and Supramolecular Polymers: Interesting Biomaterials for Drug Delivery

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