Type 3 innate lymphoid cells as an indicator of renal dysfunction and serum uric acid in hyperuricemia

Abstract

Background. Type 3 innate lymphoid cells (ILC3s) are a newly identified group of innate immune cells that participate in the progression of several metabolic diseases by secreting interleukin (IL)-17 and IL-22. These cytokines are associated with hyperuricemia (HUA) severity and development; however, the relationship between ILC3s and HUA remains unclear.Objectives. To determine the characteristics of circulating ILC3s in patients with HUA.Materials and methods. Type 3 innate lymphoid cells and their subsets were detected using flow cytometry in peripheral blood mononuclear cells (PBMCs) of 80 HUA patients and 30 healthy controls (HC). Plasma levels of IL-17A and IL-22 were measured with enzyme-linked immunosorbent assay (ELISA). Clinical data of enrolled subjects were collected from electronic medical records. Results.In patients with HUA, the frequency of circulating ILC3s was elevated and positively correlated with levels of serum uric acid and serum creatinine (Scr). Although there was no significant difference in the plasma concentration of IL-17A between the patients with HUA and healthy controls, positive correlations between plasma IL-17A and the concentration of serum uric acid and frequency of circulating ILC3s were observed in the patients with HUA. Conclusions.In patients with HUA, positive correlations were detected between circulating ILC3 levels, plasma IL-17A and serum uric acid. Therefore, ILC3s and IL-17A may be useful indicators of disease severity, and are potential new therapeutic targets in HUA.

Washed microbiota transplantation promotes homing of group 3 innate lymphoid cells to the liver via the CXCL16/CXCR6 axis: a potential treatment for metabolic-associated fatty liver disease

“…Previous studies have implicated ILC3s in various metabolic and chronic liver diseases. 21 , 33 , 34 In patients with type 2 diabetes, the frequency of peripheral ILC3s is significantly lower than that in healthy individuals, and there is a negative correlation between the levels of fasting glucose and glycated hemoglobin and the frequency of peripheral ILC3s. 34 In contrast, our previous study revealed that patients with hyperuricemia present significantly higher levels of ILC3s in PBMCs, which are positively correlated with disease severity.…”

“… 34 In contrast, our previous study revealed that patients with hyperuricemia present significantly higher levels of ILC3s in PBMCs, which are positively correlated with disease severity. 33 Moreover, Wang et al observed a higher prevalence of circulating ILC3s among individuals with cirrhosis, a trend that further exacerbates as the disease advances. 21 In the present study, abnormal distribution of ILC3s was observed in human and murine MAFLD.…”

The dysregulation of immune cells induced by uric acid: mechanisms of inflammation associated with hyperuricemia and its complications

“…Therefore, ILC3s and IL-17A could serve as valuable indicators of disease severity and potential novel therapeutic targets for HUA. It has also been demonstrated that these ILC3 cytokines are associated with the severity and progression of HUA ( 109 ).…”