Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endo- to ecodormancy in apple buds

Cattani, Amanda Malvessi; Falavigna, Vítor da Silveira; Silveira, Carolina Pereira; Buffon, Vanessa; Maraschin, Felipe dos Santos; Pasquali, Giancarlo; Revers, L. F.

doi:10.1007/s00299-020-02595-z

Cited by 11 publications

(9 citation statements)

References 66 publications

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“…This group of genes was differentially expressed during dormancy progression in peach and apricot ( Yu et al, 2020 ). From the cytokinin signaling pathway, the type-B cytokinin response regulators BRR1, 7, 8, 9 , and 10 were included in this study, as they were found to play a role in the endodormancy to ecodormancy transition in apple previously ( Cattani et al, 2020 ). Not only hormones but also respiratory stress has been proposed to play a role in dormancy release ( Vimont et al, 2019 ); therefore, the gene Respiratory burst oxidase protein F (RBOH, MD14G1211700) was included, which is differentially expressed in cherry, peach, and apricot, as well as Md_peroxidase , mentioned above).…”

Section: Resultsmentioning

confidence: 99%

Time-Resolved Analysis of Candidate Gene Expression and Ambient Temperature During Bud Dormancy in Apple

2022

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Winter dormancy – a period of low metabolic activity and no visible growth – appears as an adaptation to harsh winter conditions and can be divided into different phases. It is tightly controlled by environmental cues, with ambient temperature playing a major role. During endodormancy, a cultivar-specific amount of cold needs to be perceived, and during ecodormancy, heat hours accumulate before bud burst and anthesis in spring. Expression analysis, performed in several key fruit tree species, proved to be very useful in elucidating the molecular control of onset and release of dormancy. However, the time resolution of these experiments has been limited. Therefore, in this study, dense time-series expression analysis was conducted for 40 candidate genes involved in dormancy control, under the cool-temperate climate conditions in Dresden. Samples were taken from the cultivars ‘Pinova’ and ‘Gala,’ which differ in flowering time. The set of candidate genes included well-established dormancy genes such as DAM genes, MdFLC-like, MdICE1, MdPRE 1, and MdPIF4. Furthermore, we tested genes from dormancy-associated pathways including the brassinosteroid, gibberellic acid, abscisic acid (ABA), cytokinin response, and respiratory stress pathways. The expression patterns of well-established dormancy genes were confirmed and could be associated with specific dormancy phases. In addition, less well-known transcription factors and genes of the ABA signaling pathway showed associations with dormancy progression. The three ABA signaling genes HAB1_chr15, HAI3, and ABF2 showed a local minimum of gene expression in proximity of the endodormancy to ecodormancy transition. The number of sampling points allowed us to correlate expression values with temperature data, which revealed significant correlations of ambient temperature with the expression of the Malus domestica genes MdICE1, MdPIF4, MdFLC-like, HAB1chr15, and the type-B cytokinin response regulator BRR9. Interestingly, the slope of the linear correlation of temperature with the expression of MdPIF4 differed between cultivars. Whether the strength of inducibility of MdPIF4 expression by low temperature differs between the ‘Pinova’ and ‘Gala’ alleles needs to be tested further.

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Section: Resultsmentioning

confidence: 99%

Time-Resolved Analysis of Candidate Gene Expression and Ambient Temperature During Bud Dormancy in Apple

2022

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“…S10 a). Recently, it was proposed that CK stimuli repress MdDAM1 expression (Cattani et al ., 2020 ), suggesting that a negative feedback loop between MADS TFs and CKs may exist. MdGA2ox1 ( GA2‐oxidase 1 ; MD05G1207000) encodes an enzyme responsible for the deactivation of bioactive gibberellin (GA), preventing the accumulation of this growth‐promoter hormone during dormancy (Yamaguchi, 2008 ; Beauvieux et al ., 2018 ; Fadón et al ., 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Unraveling the role of MADS transcription factor complexes in apple tree dormancy

et al. 2021

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 A group of MADS transcription factors (TFs) are believed to control temperature-mediated bud dormancy. These TFs are encoded by genes similar to SHORT VEGETATIVE PHASE (SVP) from Arabidopsis and called DORMANCY-ASSOCIATED MADS-BOX (DAM). MADS proteins form transcriptional complexes whose combinatory composition define their molecular function. However, how MADS multimeric complexes control the dormancy cycle in trees is unclear.  Apple MdDAM and other dormancy-related MADS proteins form complexes with MdSVPa, which is essential for the transcriptional complexes ability to bind to DNA. Sequential DNA-affinity purification sequencing (seq-DAP-seq) was performed to define the genome-wide binding sites of apple MADS TF complexes. Target genes associated with the binding sites were identified by combining seq-DAP-seq data with transcriptomics datasets obtained by the glucocorticoid receptor system, and RNA-seq data related to apple dormancy.  We have determined a gene regulatory network (GRN) formed by MdSVPa-containing complexes, which regulate the dormancy cycle in response to environmental cues and hormonal signaling pathways. Moreover, novel molecular evidence on the evolutionary functional segregation between DAM and SVP proteins in the Rosaceae is presented. MdSVPa sequentially forms complexes with the MADS TFs that predominate at each dormancy phase, altering its DNA-binding specificity and, therefore, the transcriptional regulation of its target genes.

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“…Overexpression of PmDAM6 increases the ABA content in the late dormancy and bud breaking stages, while decreasing CK level in Prunus [121]. CK-triggered responses down-regulate MdoDAM1 via MdoBRRs, which further reduces ABA content and promotes the release of bud dormancy [122]. In addition to DAM hub, the enhancement of light-mediated CKs signals further negatively regulates ABA content in the dormant bud, indicating that light signal is also involved in the cross-talk between CKs and ABA [123][124][125][126].…”

Section: Aba and Cytokininsmentioning

confidence: 99%

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

Pan

Liang

Sui

et al. 2021

Genes

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Bud dormancy is an evolved trait that confers adaptation to harsh environments, and affects flower differentiation, crop yield and vegetative growth in perennials. ABA is a stress hormone and a major regulator of dormancy. Although the physiology of bud dormancy is complex, several advancements have been achieved in this field recently by using genetics, omics and bioinformatics methods. Here, we review the current knowledge on the role of ABA and environmental signals, as well as the interplay of other hormones and sucrose, in the regulation of this process. We also discuss emerging potential mechanisms in this physiological process, including epigenetic regulation.

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Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endo- to ecodormancy in apple buds

Cited by 11 publications

References 66 publications

Time-Resolved Analysis of Candidate Gene Expression and Ambient Temperature During Bud Dormancy in Apple

Time-Resolved Analysis of Candidate Gene Expression and Ambient Temperature During Bud Dormancy in Apple

Unraveling the role of MADS transcription factor complexes in apple tree dormancy

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

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