Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells

Chen, C. W.; Tsai, Yu‐Hui; Deng, Win Ping; Shih, Shang-Wu; Fang, Chia Lang; Burch, Jennie; Chen, W. H.; Lai, Wen-FuThomas

doi:10.1016/j.orthres.2004.09.002

Cited by 92 publications

(70 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously investigated the signaling pathways involved in chondrogenesis under the control of type I and type II collagen, TGF␤1, and platelet-rich plasma (3,4,32,33,38). Building on the observations from our previous studies, we established a coculture system in which MSCs were directed toward chondrogenesis under the influence of mature chondrocytes, thereby creating a better simulation of physiologic conditions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro stage‐specific chondrogenesis of mesenchymal stem cells committed to chondrocytes

Chen

Lai

et al. 2009

Arthritis & Rheumatism

110

118

View full text Add to dashboard Cite

Objective. Osteoarthritis is characterized by an imbalance in cartilage homeostasis, which could potentially be corrected by mesenchymal stem cell (MSC)-based therapies. However, in vivo implantation of undifferentiated MSCs has led to unexpected results. This study was undertaken to establish a model for preconditioning of MSCs toward chondrogenesis as a more effective clinical tool for cartilage regeneration.Methods. A coculture preconditioning system was used to improve the chondrogenic potential of human MSCs and to study the detailed stages of chondrogenesis of MSCs, using a human MSC line, Kp-hMSC, in commitment cocultures with a human chondrocyte line, hPi (labeled with green fluorescent protein [GFP]). In addition, committed MSCs were seeded into a collagen scaffold and analyzed for their neocartilage-forming ability.Results. Coculture of hPi-GFP chondrocytes with Kp-hMSCs induced chondrogenesis, as indicated by the increased expression of chondrogenic genes and accumulation of chondrogenic matrix, but with no effect on osteogenic markers. The chondrogenic process of committed MSCs was initiated with highly activated chondrogenic adhesion molecules and stimulated cartilage developmental growth factors, including members of the transforming growth factor ␤ superfamily and their downstream regulators, the Smads, as well as endothelial growth factor, fibroblast growth factor, insulin-like growth factor, and vascular endothelial growth factor. Furthermore, committed Kp-hMSCs acquired neocartilageforming potential within the collagen scaffold. Osteoarthritis (OA), one of the most common musculoskeletal diseases, has been ascribed to an imbalance in cartilage homeostasis in the aging process (1,2). Hyaline cartilage has little capacity for self-repair. As a result, continuous mechanical stress can lead to the degradation of articular cartilage, culminating in a vicious cycle of destructive processes (3). Many therapeutic interventions directed at the restoration of the reparative capacity of chondrocytes have been explored (3-5). One of the emerging approaches is cell-based therapy, in which expanded chondrocytes are used for cartilage repair. However, the outcome of this approach has been disappointing, due to the difficulties in maintaining chondrocytic phenotypes and the decrease in proliferative capacity of autologous chondrocytes with increasing age (1,6,7). Conclusion. These findings help define the molecular markers of chondrogenesis and more accuratelyCell therapy based on autologous mesenchymal stem cells (MSCs), which have a vast proliferative ca-

show abstract

Section: Discussionmentioning

confidence: 99%

“…We have also studied the expression of matrix proteins, the type I and type II collagens, which induce chondrogenesis in human MSCs under the influence of TGF␤1 (3). In this study, we more accurately delineate the stages and define the molecular markers that are involved in chondrocytic differentiation of MSCs.…”

mentioning

confidence: 99%

In vitro stage‐specific chondrogenesis of mesenchymal stem cells committed to chondrocytes

Chen

Lai

et al. 2009

Arthritis & Rheumatism

110

118

View full text Add to dashboard Cite

show abstract

“…Specific concentrations have been shown to increase fibroblast proliferation, increase collagen production, glycosaminoglycan expression, and encourage MSC's to differentiate into fibroblasts (Spindler, Imro et al 1996;Locklin, Oreffo et al 1998;Meaney Murray, Rice et al 2003;Chen, Tsai et al 2004;Chen, Tsai et al 2005;Moreau, Chen et al 2005;Giannouli and Kletsas 2006;Marenzara, Wilson-Jones et al 2006). Table 7 summarises the effects that different concentrations of TGF-β1 have had on fibroblasts and MSC's.…”

Section: In Vitro Studies With Tgf-β1mentioning

confidence: 99%

Tissue Engineering of Ligaments

Rathbone¹,

Cartmell²

2011

Tissue Engineering for Tissue and Organ Regeneration

View full text Add to dashboard Cite

“…Stem cellbased therapies that harmonize with tissue-engineering technologies, and biomaterials are vital for the continuous advance of cartilage regenerative medicine [2,3].…”

Section: Introductionmentioning

confidence: 99%

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fuentes-Mera¹,

Camacho²,

Moncada‐Saucedo³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

View full text Add to dashboard Cite

Articular cartilage injuries caused by traumatic/mechanical progressive degeneration result in joint pain, swelling, the consequent loss of joint function, and eventually osteoarthritis. Articular tissue possesses a poor ability to regenerate that further complicates the therapeutic approaches. Mesenchymal stem cells (MSCs) have emerged as a promising alternative treatment. Recently, it has been reported that a wide variety of strategies ranging from merely using cells in the injured area to employ biofunctional substitutes in which cells are harmonizing with scafolding and growth factors to create an engineered cartilage tissue.This chapter reviews the state-of the-art in cartilage tissue engineering focused on tissue engineering approaches designed to recapitulate the native development of cartilage and its tridimensional structure as an osteochondral unit. Since the production of hypertrophied tissue is one of the most critical challenges to overcome in chondral tissue regeneration, here we show new strategies to minimize hypertrophy in cartilage. Finally, the eicacy and safety of diferent treatments of cartilage in current clinical trials will be discussed.While the framework provides new features and beneits concerning the strategies for articular tissue regeneration, this chapter presents a set of tools to improve approaches to orthopedic regenerative medicine based on the use of MSCs.Keywords: MSCs, MSCs-subpopulations, cartilage regeneration, cartilage tissue engineering, hypertrophy © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionThe chondrocytes are the only cells found in cartilage. The chondrocytes demonstrate distinctive properties such as being metabolically active in order to maintain the renewal of the extracellular matrix (ECM) by synthesizing collagens, proteoglycans, hyaluronic acid, and glycoproteins. Restoration of the cartilage damage is still challenging for orthopedic medicine due to its poor ability to regenerate [1].Mesenchymal stem cells (MSCs) have potential applications in tissue engineering, and regenerative medicine represents an atractive option for repairing lesions in cartilage. Stem cellbased therapies that harmonize with tissue-engineering technologies, and biomaterials are vital for the continuous advance of cartilage regenerative medicine [2,3].Once the relationship between structure function in normal and damaged tissues is understood and the development of biological substitutes for the repair or regeneration can be reached. To develop a biological substitute, tissue engineering uses scafolds, cells, and growth factors. Each of these elements alone is able to promote tissue regeneration, but composites fabricated in combination would be more efective [4,5].The objective of the present chapter is, therefore, t...

show abstract

Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells

Cited by 92 publications

References 41 publications

In vitro stage‐specific chondrogenesis of mesenchymal stem cells committed to chondrocytes

In vitro stage‐specific chondrogenesis of mesenchymal stem cells committed to chondrocytes

Tissue Engineering of Ligaments

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Contact Info

Product

Resources

About