Type I fimbriae mediate in vitro adherence of porcine F18ac+ enterotoxigenic Escherichia coli (ETEC)

Duan, Qiangde; Nandre, Rahul; Zhou, Mingxu; Zhu, Guoqiang

doi:10.1007/s13213-017-1305-z

Cited by 9 publications

(8 citation statements)

References 39 publications

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“…AIDA-1 is associated with EAST-1 and ST genes and usually detected in F18 + E. coli (Duan et al, 2017). In the present study, detection frequency of AIDA-1 was 26.5% in F18 + E. coli, which was significantly higher than that (4.4%) in F4 + E. coli.…”

Section: Discussioncontrasting

confidence: 56%

Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

Do¹,

Byun²,

Lee³

2019

PVJ

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Ninety-one F4 + pathogenic Escherichia (E.) coli and 181 F18 + pathogenic E. coli were isolated from piglets suffering enteric colibacillosis during 2007-2016. These strains were analyzed for O-serogroups, adhesin genes (eae, paa, AIDA-1), toxin genes (LT, STa, STb, Stx2e, EAST-1), and their susceptibility to 16 antimicrobials using disc diffusion method. We found that O149 and O139 were predominant serogroups in F4 + E. coli (36.3%) and F18 + E. coli (16.6%), respectively. AIDA-1 was the most predominant adhesin gene in F18 + E. coli (26.5%) while paa was the most predominant adhesin gene in F4 + E. coli (30.8%). LT (70.3%), STb (84.6%), and EAST-1 (73.6%) were detected with high frequency in F4 + E. coli. However, STa (43.6%) and Stx2e (49.2%) were the predominant toxin genes detected in F18 + E. coli. Both F4 + and F18 + E. coli showed high resistance to tetracycline (F4 + : 91.2%, F18 + : 90.6%), chloramphenicol (F4 + : 87.9%, F18 + : 92.3%), and streptomycin (F4 + : 89.0%, F18 + : 84.0%). F18 + E. coli showed higher resistance to colistin (9.4%) rather than F4 + E. coli (2.2%). In summary, we compared serogroups, virulence factors, and antimicrobial susceptibility of F4 + and F18 + E. coli from diarrheic weaned piglets. Results of this study could be used to design control measures for enteric colibacillosis in piggeries.To Cite This Article: Do KH, Byun JW and Lee WK, 2019. Serogroups, virulence genes and antimicrobial resistance of F4 + and F18 + Escherichia coli isolated from weaned piglets. Pak Vet J, 39(2): 266-270. http://dx.

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Section: Discussioncontrasting

confidence: 56%

Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

Do¹,

Byun²,

Lee³

2019

PVJ

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“…Of the Tfp biogenesis genes, ten were more highly expressed in smooth biofilm (5 passing both significance cutoffs), including pilX , a minor pilin (Tables 1 and S1). These gene expression differences correlate with published adherence functions for their encoded proteins (19, 20) and with our observations that the smooth biofilms are more strongly attached to polystyrene surfaces than rough biofilms.…”

Section: Resultssupporting

confidence: 88%

In vitrophenotypic and transcriptomic variation inNeisseria musculimorphotypes correlate with colonization variability and persistencein vivo

Thapa

Lauderback

Simmons

et al. 2022

Preprint

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Asymptomatic colonization of the upper respiratory tract is a common trait of the two human restricted pathogens, Neisseria gonorrhoeae, and Neisseria meningitidis. In vivo models of pathogenic neisserial infections are heterologous systems that permit short-term colonization but do not fully recapitulate infections in humans. Studying Neisseria musculi (Nmus), an oral commensal, in laboratory mice allows investigation of Neisseria-host interactions that avoids host restriction barriers. Nmus produces smooth and rough morphotypes on solid media. We compared the in vitro phenotypes, biofilm transcriptomes, in vivo colonization patterns, and burdens of the two Nmus morphotypes. We observed that the two morphotypes differ in biofilm formation, pilin production, transformation frequency, and aggregation in vitro. These phenotypes strongly correlated with differential expression of a set of genes in the Nmus biofilms including those that encoded factors for bacterial attachment. In vivo, the smooth morphotype stably colonized the oral cavities of all inoculated A/J and C57BL/6J mice at higher burdens relative to the rough. Interestingly, both morphotypes colonized the oral cavities of A/Js at higher magnitudes than in C57BL/6Js. Gut colonization by the smooth morphotype was qualitatively higher than the rough. Nasal colonization in the A/Js was transient following nasal inoculations. Collectively, our results demonstrate that colonization by Nmus can be affected by various factors including Nmus morphotypes, inoculation routes, anatomical niches, and host backgrounds. The Nmus-mouse model can use variable morphotype-host combinations to study the dynamics of neisserial asymptomatic colonization and persistence in multiple extragenital niches.

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“…Fimbriae play an important role in allowing E. coli to attach to the intestinal mucosa and epithelial cells [ 36 , 37 ]. In late 1990s, the most predominant fimbriae in Korean pigs was F6, which then changed to F5 in the mid-2000s [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance, virulence genes, and phylogenetic characteristics of pathogenic Escherichia coli isolated from patients and swine suffering from diarrhea

Seo

Lee

2022

BMC Microbiol

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Background Escherichia (E.) coli causes colibacillosis in swine and humans, and is frequently associated with antimicrobial resistance. In this study we aimed to compare antimicrobial resistance, O-serogroups, virulence genes, and multi-locus sequence type of E. coli between isolates from pigs and patients suffering from diarrhea, and the most prevalent pathogenic E. coli strain from swine isolates in Korea. Methods We tested 64 and 50 E. coli strains from pigs and patients suffering from diarrhea for antimicrobial susceptibility test, virulence genes, O-serogroups, and multi-locus sequence typing. Results We confirmed that isolates from swine showed significantly higher resistance than from those from patients, especially to fluoroquinolone (ciprofloxacin: 37.5 and 10.0%; norfloxacin: 29.7 and 8.0%, respectively). Stx1 (46.0%) was most frequently detected in patients followed by stx2 (38.0%). There was no significant difference in stx2 (swine: 23.4%, patients: 38.0%). In isolates from patients, O157 (12.0%) was the most prevalent O-serogroup, and two isolates (3.1%) from pigs were confirmed to have O157. Additionally, sequence type (ST) 10 (swine: 6 isolates, patients: 2 isolates) and ST 88 (swine: 2 isolates, patients: 1 isolate) were simultaneously detected. Conclusions We found that both isolates from swine and human had the stx2 gene, which could cause severe disease. Moreover, antimicrobial resistance was significantly higher in pigs than in patients. These results suggest that pig could act as a reservoir in human infection and antimicrobial resistance could be transferred to human from pigs.

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Type I fimbriae mediate in vitro adherence of porcine F18ac+ enterotoxigenic Escherichia coli (ETEC)

Cited by 9 publications

References 39 publications

Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

In vitrophenotypic and transcriptomic variation inNeisseria musculimorphotypes correlate with colonization variability and persistencein vivo

Antimicrobial resistance, virulence genes, and phylogenetic characteristics of pathogenic Escherichia coli isolated from patients and swine suffering from diarrhea

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