Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin

Abstract

Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of Acipenser baerii and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (Td) at 22.5 and melting temperature (Tm) at 72.5 °C. Coating SCC potentiated proliferation, migration, and invasion of human dermal fibroblast adult (HDFa) cells. Furthermore, SCC upregulated the gene expression of extracellular matrix (ECM) components (col Iα1, col IIIα1, elastin, and Has2) and epithelial-mesenchymal transition (EMT) molecules (N-cadherin, Snail, and MMP-1) in HDFa. Pretreatment with Akt and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated the HDFa invasion caused by SCC. In mice, the application of SCC on dorsal wounds effectively facilitated wound healing as evidenced by 40–59% wound contraction, whereas the untreated wounds were 18%. We observed that SCC reduced inflammation, promoted granulation, tissue formation, and ECM deposition, as well as re-epithelialization in skin wounds. In addition, SCC markedly upregulated the production of growth factors in the dermis, and dermal and subcutaneous white adipose tissue; in contrast, the administration of tilapia skin collagen (TSC) characterized by typical type I collagen was mainly expressed in the epidermis. Collectively, these findings indicate SCC accelerated wound healing by targeting fibroblast in vitro and in vivo.

Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects

“…Therefore, based on the information reported in several studies [3,[12][13][14]24,50,51,56,[74][75][76]79,82,90,95,102,[106][107][108][113][114][115][116][117][118][119]127,128,[130][131][132][133], the composition of main and minor collagen types in ECM to consider the quality of a repaired cartilage as hyaline-type cartilage should be:…”

“…The mature articular cartilage comprises more than 90-95% of cartilage collagen. Type II and type XI collagen co-polymerize with type IX collagen to form a heteropolymeric fibrillar framework that gives cartilage its tensile strength [3,50,51].…”

Novel Antioxidant Collagen Peptides of Siberian Sturgeon (Acipenserbaerii) Cartilages: The Preparation, Characterization, and Cytoprotection of H2O2-Damaged Human Umbilical Vein Endothelial Cells (HUVECs)

“…In the receiving process of sturgeon eggs, cartilage, which accounts for 10% of the sturgeon’s weight, becomes a by-product. Therefore, active substances in sturgeon cartilage, such as chondroitin sulfate [ 38 ], collagen [ 39 , 40 ], and anti-inflammatory peptides [ 41 ], were studied constantly to replace shark cartilage, which is used in health and functional products. The Siberian sturgeon, Acipenser baerii Brandt, inhabits large Siberian rivers from the Ob to the Kolyma and Lake Baikal, and it is one of the important breeding varieties in China.…”

Treatment of Acute Wounds With Recombinant Human-Like Collagen and Recombinant Human-Like Fibronectin in C57BL/6 Mice Individually or in Combination

“…In the inflammatory stage, collagen will produce a strong and rapid inflammatory response, which is temporary and will subside soon, so as to promote wound healing. In the proliferative phase, on the one hand, collagen plays a chemotactic role, which significantly promotes the adhesion and effectively increases the proliferation of FN around the wound ( Huang et al, 2012 ; Smithmyer et al, 2014 ; Lai et al, 2020 ). On the other hand, it can activate macrophages, induce the expression of various cytokines and growth factors, and promote angiogenesis ( Lin et al, 2006 ; To and Midwood, 2011 ; Elbialy et al, 2020 ).…”