Type II epithelial cells from the lung of Syrian hamsters: Isolation and metabolism

Pfleger, R.C.

doi:10.1016/0014-4800(77)90027-2

Cited by 28 publications

(4 citation statements)

References 18 publications

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“…Yoneda (1974), proteolytic dispersion of lung cells has been the mainstay of isolating alveolar epithelial cells. Proteolytic enzymes used include protease mixture (Finkelstein and Shapiro, 1982;Massey et al, 1987), trypsin (Kikkawa and Yoneda, 1974;Fisher and Furia, 1977;Mason et al, 1977;Funkhouser et al, 1987;Augustin-Voss et al, 1989;Pfleger, 1977), collagenase (Wilson et al, 1986) and elastase (Dobbs et al, 1980;Dobbs et al, 1986;Goodman and Crandall 1982;Sikpi et al, 1986;Robinson et al, 1984;Brown et al, 1984;Cheek et al, 1989).…”

Section: Primary Culture Of Alveolar Pneumocytesmentioning

confidence: 97%

“…These include unit gravity separation or centrifugal elutriation (Brown et al, 1984;Wilson et al, 1986;Massey et al, 1987), centrifugation of the crude cell mixture on a discontinuous gradient of percoll (Skilrud and Martin, 1984;Richards et al, 1987;Augustin-Voss et al, 1989), ficoll (Kikkawa and Yoneda, 1974;Fisher and Furia, 1977;Kikkawa et al, 1975;Finkelstein and Shapiro, 1982;Pfleger, 1977), or metrizamide (Dobbs et al, 1980;Goodman and Crandall, 1982;Cheek et al, 1989;Sikpi et al, 1986;Robinson et al•, 1984), and removal of blood-borne cells adhering to leukocyte-common antigen (Weller and Karnovsky, 1986a;Banks et al, 1991) or IgG (Dobbs et al, 1986;Banks et al, 1991) coated on a hydrophobic surface. The IgG panning method may be the least time-consuming.…”

Section: Primary Culture Of Alveolar Pneumocytesmentioning

confidence: 99%

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Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Kim

Suh

Lubman

et al. 1992

Journal of Tissue Culture Methods

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SUMMARY: To investigate the celt physiologic and biological properties of the alveolar epithelium, we studied rat alveolar epithelial cell monolayers grown on permeable supports in primary culture. Type II alveolar epithelial cells were disaggregated using elastase, and partially purified on a discontinuous metrizamide gradient. These isolated cells were plated onto tissue culture-treated Nuclepore membrane filters at 1.5 )< 106 cells/cm 2 and maintained in a humidified incubator (5% CO2 in air, 37 ° C). After 2 days in culture, the bathing media on both sides of the cell monolayers were changed to fresh culture medium, thus removing nonadherent cells (mostly lcukocytes). These monolayers exhibit a high transmonolayer resistance (>2000 ~-cm 2) and actively transport ions. Radionuclide flux studies indicate that Na + is the predominant ionic species absorbed actively under baseline conditions, accounting for about 80% of the total active ion transport. CI-seems to be passively transported across the epithelium. However, when the epithelium is exposed to a beta-agonist (terbutaline), active absorption of Na + is increased ar, d active absorption of C1-occurs. Ahhough it is clear that both active Na + and CI-transport are dependent on Na+/K+-ATPase activity, and that Na + enters cells predominantly through channels, the specific mechanisms by which CI-enters and exits the alveolar epithelial cells remain unclear. The stimulated reabsorption of Na + and CImay be important in helping to remove excess fluid from alveolar air spaces in the lung.

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Section: Primary Culture Of Alveolar Pneumocytesmentioning

confidence: 97%

Section: Primary Culture Of Alveolar Pneumocytesmentioning

confidence: 99%

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Kim

Suh

Lubman

et al. 1992

Journal of Tissue Culture Methods

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“…Methods for the isolation of type II cells were first developed by Kikkawa and Yoneda (1974). These methods and subsequent modifications have been utilized to isolate type II cells from the adult rat (Dobbs et al, 1980;Dobbs et al, 1986a;Fisher and Furia, 1977;Fisher et al, 1980), mouse (Bortnick et al, 2003), rabbit (Finkelstein and Shapiro, 1982;Kikkawa et al, 1975), human (Robinson et al, 1984), guinea pig (Sikpi et al, 1986), hamster (Pfleger, 1977;, and bovine (Augustin-Voss et al, 1989) lung, as well as from fetal rat Kresch et al, 1987), rabbit (Aeberhard et al, 1985), and human lung.…”

Section: Introductionmentioning

confidence: 97%

Lung Lipid Composition and Surfactant Biology

Fisher¹

2015

Comparative Biology of the Normal Lung

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“…This model has been used to study the biochemistry of surfactant production (30-32) and could well be applied to the study of pulmonary toxicology. Although there are several cell culture systems for the study of Type II pneumonocytes (33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43) The dissociation of fetal tissue into single cells and its subsequent reaggregation into histotypic structures was first described by Moscona (44,45). Histotypic reaggregation has been described for fetal heart (46), brain (47), kidney (48) and liver (49).…”

Section: Introductionmentioning

confidence: 99%

Organotypic Culture of Fetal Lung Type II Alveolar Epithelial Cells: Applications to Pulmonary Toxicology

Shami¹,

Aghajanian²,

Sanders³

1984

Environmental Health Perspectives

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Techniques for isolation and culture of fetal lype II alveolar epithelial cells, as well as the morphologic and biochemical characteristics of these histotypic cultures, are described.Type II alveolar epithelial cells can be isolated from fetal rat lungs and grown in an organotypic culture system as described in this review. The fetal Type II cells resemble differentiated rat Type II cells in morphology, biochemistry, and karyotype as they grow in culture for up to 5 weeks. The cells of the mature organotypic cultures form alveolarlike structures while growing on a gelatin sponge matrix. The Type II cells also synthesize and secrete pulmonary surfactant similar in biochemical composition to that produced in vivo. This system has been used to study the effects of hormones on surfactant production and composition. The organotypic model has many potential applications to the study of pulmonary toxicology.

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Type II epithelial cells from the lung of Syrian hamsters: Isolation and metabolism

Cited by 28 publications

References 18 publications

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers

Lung Lipid Composition and Surfactant Biology

Organotypic Culture of Fetal Lung Type II Alveolar Epithelial Cells: Applications to Pulmonary Toxicology

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