Type XIV Collagen

Manon‐Jensen, Tina; Karsdal, M.A.

doi:10.1016/b978-0-12-809847-9.00014-3

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…[36][37][38][39][40] The high levels of collagen XIV in KC are notable because this collagen is known to be upregulated in areas of high mechanical stress. 41 Local mechanical stress on the wound/scar is thought to be an important risk factor for the development and progression of keloids. 42 The abnormal abundance of collagen VI in keloids has been observed previously.…”

Section: Discussionmentioning

confidence: 99%

Keloidal Collagen May Be Produced Directly by αSMA-positive Cells: Morphological Analysis and Protein Shotgun Analysis

Kaku

Ichinose

Dohi

et al. 2023

Plastic and Reconstructive Surgery - Global Open

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Background: Keloids are fibroproliferative lesions caused by abnormal dermal wound healing. Keloidal collagen (KC) is a pathognomic feature of keloids, but the mechanism by which it forms is unknown. This study aimed to evaluate the histopathology of KC and thereby gain clues into how it forms. Methods: The cross-sectional study cohort consisted of a convenience series of patients with keloids who underwent surgical excision. Skin pieces (3 mm2) were collected from the keloid center and nearby control skin. Histopathology was conducted with light and electron microscopy and immunohistochemistry. KC composition was analyzed with protein shotgun analysis. Results: Microscopic analyses revealed the ubiquitous close association between KC and αSMA-positive spindle-shaped cells that closely resembled myofibroblasts. Neither KC nor the spindle-shaped cells were observed in the control tissues. Compared with control skin, the collagen fibers in the KC were overall thinner, their diameter varied more, and their spacing was irregular. These features were particularly pronounced in the collagens in the vicinity of the spindle-shaped cells. Protein shotgun analysis did not reveal a specific collagen in KC but showed abnormally high abundance of collagens I, III, VI, XII, and XIV. Conclusions: These findings suggest that KC may be produced directly by myofibroblasts rather than simply being denatured collagen fibers. Because collagens VI and XII associate with myofibroblast differentiation, and collagen XIV associates with local mechanical stress, these collagens may reflect, and perhaps contribute to, the keloid-specific local conditions that lead to the formation of KC.

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Section: Discussionmentioning

confidence: 99%

Keloidal Collagen May Be Produced Directly by αSMA-positive Cells: Morphological Analysis and Protein Shotgun Analysis

Kaku

Ichinose

Dohi

et al. 2023

Plastic and Reconstructive Surgery - Global Open

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“…Although the endochondral ossification has been widely studied ( Long and Ornitz, 2013 ), information on the genes involved in the endochondral ossification and FHS in chickens are still scarce. COL14A1 has already been associated to calcium ligation and cell morphogenesis ( Rojas-Peña et al, 2014 ), being highly expressed when submitted to mechanical stress ( Manon-Jensen and Karsdal, 2016 ). COL16A1 was associated to osteoarthritis in humans ( Karlsson et al, 2010 ; Chou et al, 2013 ), while polymorphisms in COL8A gene were associated to the loss of function during embryogenesis leading to congenital vertebral malformations ( Gray et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers

et al. 2022

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Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers.

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Multiscale analysis of human tissue engineered matrices for next generation heart valve applications

et al. 2023

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Type XIV Collagen

Cited by 4 publications

References 15 publications

Keloidal Collagen May Be Produced Directly by αSMA-positive Cells: Morphological Analysis and Protein Shotgun Analysis

Keloidal Collagen May Be Produced Directly by αSMA-positive Cells: Morphological Analysis and Protein Shotgun Analysis

Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers

Multiscale analysis of human tissue engineered matrices for next generation heart valve applications

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