Typing of Enterococcus spp. strains in 4 hospitals in the Małopolska region in Poland

Talaga, Katarzyna; Odrowąż-Konduracka, Dalma; Paradowska, Beata; Jagiencarz-Starzec, Barbara; Wolak, Zdzisław; Bulanda, Małgorzata; Szczypta, Anna

doi:10.17219/acem/68265

Cited by 4 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be emphasized that all E. faecium isolates included in the study were resistant to ampicillin, imipenem and norfloxacin, which, unfortunately, is not unusual, since according to the epidemiological data and reports of other authors, hospital-associated E. faecium is typically resistant to ampicillin and fluoroquinolones [1,13,14,[20][21][22]. Moreover, 17 out of the 18 isolates demonstrated HLAR, including 27.7% with the HLSR and 38.8% with the HLGR; an additional 27.7% of the isolates were both HLSR and HLGR.…”

Section: Discussionmentioning

confidence: 89%

“…This observation is in line with other reports. In the study of Talaga et al [13] analysing enterococcal strains from four Polish hospitals, fluoroquinolone resistance ranged between 33.3% up to 96%. The authors also observed that ampicillin-and vancomycin resistance was demonstrated by all E. faecium isolates from the two hospitals, including 87.3% of the isolates presenting the HLAR phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteremia and abdominal infections (which are frequent sources of bacteremia/septicaemia) represent clinical presentations typical for enterococci [14]. The ICU stay, in turn, is considered an important risk factor for enterococcal infections [13,14,20]. Although during a 5-month collection period nearly an equal number of E. faecalis (n=17; 48.5%) and E. faecium (n=18; 51.4%) was found, we have observed the important role of E. faecium as an etiologic agent of HAIs outcompeting E. faecalis in two hospital wards in which we simultaneously observed the highest rate of enterococcal isolation, namely in the ICU and IMU.…”

Section: Discussionmentioning

confidence: 99%

“…There have been relatively few literature data raising the problem of enterococcal antibiotic resistance in Poland, with the most recent reports being by Chmielarczyk et al (2021) [12], Krawczyk et al (2020) [9], Kamińska et al (2019) [3], Talaga-Ćwiertnia & Bulanda (2018) [11], [13], and Gawryszewska et al (2017Gawryszewska et al ( , 2016 [14,15].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of the phenotypic and genotypic antimicrobial resistance profiles of clinically significant enterococci isolated in the Provincial Specialist Hospital in Lublin, Poland

Bogut

Mrozik

Czaja

et al. 2021

Current Issues in Pharmacy and Medical Sciences

View full text Add to dashboard Cite

The increasing significance of enterococci as healthcare-associated pathogens can be linked to their limited susceptibility to antibiotics. In this study, phenotypic and genotypic resistance profiles of 35 [n=18 E. faecium (Efm); n=17 E. faecalis (Efs)] invasive isolates cultured from hospitalized patients were analysed. Phenotypic identification was verified by the multiplex PCR targeting the 16S rDNA and the ddl genes encoding for the Efs and Efm – specific ligases. Antimicrobial susceptibility was determined using the disc diffusion method and E-tests. The high-level streptomycin resistance (HLSR), high-level gentamicin resistance (HLGR) and glycopeptide resistance was verified by amplification of the ant(6)-Ia, aac(6’)-Ie-aph(2’’)-Ia, as well as vanA and vanB genes, respectively. More than 70% of all isolates were cultured from patients in the Intensive Care and Internal Medicine Units. Blood was the predominant (77%) site of isolation. All Efm isolates were resistant to ampicillin, imipenem, and norfloxacin; 17 isolates demonstrated high-level aminoglycoside resistance (HLAR), including 27.7% with HLSR, 38.8% with HLGR and 27.7% with both phenotypes. HLAR was also common in Efs (HLSR>70%, HLGR>50%), followed by norfloxacin (64.7%) and ampicillin (11.7%) resistance. The ant(6)-Ia and aac(6’)-Ie-aph(2’’)-Ia genes were detected in >90% of the HLSR and HLGR isolates, respectively. Glycopeptide resistance was detected in 4 (22.2%) Efm isolates and mediated by the vanA gene. 19 (54.3%) isolates were multidrug resistant, including 17 (89.5%) Efm. All isolates were susceptible to linezolid. The study constitutes a contribution to the analysis of enterococcal antimicrobial resistance in Polish hospitals. The monitoring of enterococcal prevalence and antimicrobial resistance is crucial to control and prevent infections.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of the phenotypic and genotypic antimicrobial resistance profiles of clinically significant enterococci isolated in the Provincial Specialist Hospital in Lublin, Poland

Bogut

Mrozik

Czaja

et al. 2021

Current Issues in Pharmacy and Medical Sciences

View full text Add to dashboard Cite

show abstract

“…It was established that despite the general tendency of the VanA phenotype to dominate, in the Lesser Poland, E. faecium with the VanB phenotype has a larger percentage share in the VRE population than E. faecium with the VanA phenotype. All of the E. faecium isolates, including VREs, were sensitive to quinupristin/dalfopristin [68].…”

Section: Epidemiological Situation In Polandmentioning

confidence: 99%

Analysis Of Selected Genetic Traits, Phenotypes, And The Epidemiological Threat Of Enterococcus Bacteria Resistant To Vancomycin

Rogóż

Sypniewski

Bednarek

2019

Postępy Mikrobiologii - Advancements of Microbiology

View full text Add to dashboard Cite

Enterococci are Gram-positive bacteria that belong to facultative anaerobic cocci. Species belonging to the Enterococcus genus generally display little infectious potential, although they can cause serious nosocomial infections. The groups at high risk include patients with proliferative diseases, chronic liver diseases, and graft recipients. Since 1980s infections with enterococci resistant to numerous antibiotics have been observed with increasing frequency. There are two independent ways of developing resistance to vancomycin, connected with the common use of vancomycin for MRSA treatment and the non-medical use of this antibiotic. Nine phenotypes of vancomycinresistant enterococcal strains can be distinguished: VanA, VanB, VanC, VanD, VanE, VanG, VanL, VanM, VanN. These phenotypes differ at the molecular level to a different extent. Current treatments of enterococcal infections usually include drugs such as linezolid, quinupristin/dalfopristin, daptomycin, tigecycline, and chloramphenicol. Data available from Europe and other parts of the world indicate a constant increase in the number of emerging VRE isolates, as well as strains resistant to antibiotics other than vancomycin. 1. Introduction. 2. Infections with enterococci. 3. Treatment of enterococcal infections and antimicrobial resistance. 4. Development of VRE phenomenon. 5. Drugs used to control infections with VRE strains. 6. Routes of VRE spread. 7. VRE phenotypes. 8. Molecular characteristics of VRE phenotypes. 9. Epidemiological situation in the world. 10. Epidemiological situation in Poland. 11. Epidemiological situation in Europe. 12. Summary ANALIZA WYBRANYCH CECH GENETYCZNYCH, FENOTYPÓW I ZAGROŻENIA EPIDEMIOLOGICZNEGO BAKTERIAMI Z RODZAJU ENTEROCOCCUS OPORNYMI NA WANKOMYCYNĘ Streszczenie: Enterokoki to bakterie Gram-dodatnie, należące do względnie beztlenowych ziarniaków. Gatunki należące do rodzaju Entero coccus na ogół mają niewielki potencjał infekcyjny, jednak mogą wywoływać groźne zakażenia szpitalne. Do grupy podwyższonego ryzyka zalicza się pacjentów z chorobami rozrostowymi, z przewlekłymi chorobami wątroby oraz po przeszczepach. Od lat osiemdziesiątych XX w. obserwuje się pojawiające się coraz częściej zakażenia enterokokami opornymi na liczne antybiotyki. Istnieją dwie, niezależne od siebie drogi rozwoju oporności na wankomycynę, związane z powszechnym leczeniem MRSA przy pomocy wankomycyny oraz jej zastosowaniem pozamedycznym. Wśród opornych na wankomycynę szczepów enterokoków można wyróżnić 9 fenotypów: VanA, VanB, VanC, VanD, VanE, VanG, VanL, VanM, VanN. Wszystkie te fenotypy różnią się między sobą w większym lub mniejszym stopniu na poziomie molekularnym. Obecnie stosowanymi w leczeniu infekcji wywołanych enterokokami są m. in. linezolid, chinuprystyna/dalfoprystyna, daptomycyna, tigecyklina i chloramfenikol. Posiadane obecnie dane, zarówno z terenu Europy, jak i całego świata wskazują na stały wzrost ilości pojawiających się izolatów VRE, jak również opornych na antybiotyki inne niż wankomycyna.

show abstract

Antibiogram Pattern and Virulence Trait Characterization of Enterococcus Species Clinical Isolates in Eastern India: A Recent Analysis

Mohanty

Behera

2022

J Lab Physicians

View full text Add to dashboard Cite

Objective We aimed to evaluate the current antimicrobial susceptibility pattern and characterize putative virulence traits among Enterococcus species isolates from various clinical specimens in view of their increased isolation rates in both community-related and serious nosocomial infections, as well as resistance to many antibiotics. Methods Study (April 2017–March 2018) included consecutive, nonrepeated, discrete, and clinically significant isolates of enterococci. Susceptibility testing included detection of high-level aminoglycoside-resistant (HLAR) and glycopeptide-resistant enterococci (GRE). All screen-positive GRE isolates were investigated by polymerase chain reaction for species confirmation and presence of vanA/vanB genes. Virulence genes ace, asa1, cyt, efa, esp, gelE, and hyl were investigated by molecular methods. Hemolysin and biofilm production were studied using phenotypic methods. Results Of 111 isolates, 89 (80.1%), 16 (14.4%), and 6 (5.4%) were from urine, pus, and blood, respectively, consisting predominantly of E. faecalis (67, 60.4%) and E. faecium (32, 28.8%). E. hirae (5, 4.5%) was the predominant non-E. faecalis non-E. faecium isolate. Other species were E. durans (4, 3.6%), E. avium (2, 1.8%), and E. mundtii (1, 0.9%). Seven (6.3%) out of the 111 isolates were GRE, all vanA genotype. HLAR was observed in 70 (63.1%) isolates, significantly higher in E. faecium than E. faecalis (81.2 vs. 58.2%; p < 0.05). All were susceptible to daptomycin. Hemolysin activity and biofilm production were observed in 38 (34.2%) and 36 (32.4%) isolates. Most frequent virulence genes were efa (77, 69.4%), ace (71, 63.9%), asa1 (67, 60.3%), and gelE (66, 59.4%). There was a predominant association of esp and hyl genes with E. faecium and that of the other genes with E. faecalis. Conclusion The study will contribute to the existing limited data on virulence trait characterization of clinical E. spp. isolates in India. At the same time, it will help to serve as a guide in the choice of empirical therapy in enterococcal infections leading to favorable clinical outcomes by decreasing the clinical failure, microbiological persistence, and associated mortality, and will lead to future studies on controlling the spread of virulent and multiresistant isolates.

show abstract

Typing of Enterococcus spp. strains in 4 hospitals in the Małopolska region in Poland

Abstract: Background. In the Małopolska province, the first case of vancomycin resistant enterococci (VRE) occurrence was an outbreak in 2001 caused by strains of the genus E. faecium carrying the vanA operon.

Cited by 4 publications

References 21 publications

Analysis of the phenotypic and genotypic antimicrobial resistance profiles of clinically significant enterococci isolated in the Provincial Specialist Hospital in Lublin, Poland

Analysis of the phenotypic and genotypic antimicrobial resistance profiles of clinically significant enterococci isolated in the Provincial Specialist Hospital in Lublin, Poland

Analysis Of Selected Genetic Traits, Phenotypes, And The Epidemiological Threat Of Enterococcus Bacteria Resistant To Vancomycin

Antibiogram Pattern and Virulence Trait Characterization of Enterococcus Species Clinical Isolates in Eastern India: A Recent Analysis

Contact Info

Product

Resources

About