Typing of sequential bacterial isolates by pulsed-field gel electrophoresis

Hartstein, Alan I.; Chetchotisakd, Ploenchan; Phelps, Charles L.; LeMonte, Ann M.

doi:10.1016/0732-8893(95)00139-8

Cited by 18 publications

(13 citation statements)

References 15 publications

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“…The pattern obtained by pulsed-field gel electrophoresis ( 9 ), using the rare-cutting enzyme Nhe I, (data not shown), was identical to patterns found among meningococci X strains isolated in United Kingdom and belonging to ST-750, clonal group X-II ( 1 ). In particular, ST-2888 resembles, except for gdh gene sequence, ST-2317, which was found among the X meningococci isolated in the United Kingdom in 2002 with phenotype X:4:P1.7 (http://pubmlst.org/neisseria).…”

supporting

confidence: 56%

Neisseria meningitidisSerogroup X Sequence Type 2888, Italy

Fazio¹,

Starnino²,

Soldà³

et al. 2010

Emerg. Infect. Dis.

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supporting

confidence: 56%

Neisseria meningitidisSerogroup X Sequence Type 2888, Italy

Fazio¹,

Starnino²,

Soldà³

et al. 2010

Emerg. Infect. Dis.

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“…PFGE was performed as already described [13]. Briefly, the bacterial DNAs were digested with 30 U of the restriction endonuclease Nhe I (NewEngland, Biolabs, USA) overnight at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Serogroup C meningococci in Italy in the era of conjugate menC vaccination

et al. 2009

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BackgroundTo assess changes in the pattern of Invasive Meningococcal Disease (IMD) in Italy after the introduction of conjugate menC vaccine in the National Vaccine Plan 2005–2007 and to provide information for developing timely and appropriate public health interventions, analyses of microbiological features of isolates and clinical characteristics of patients have been carried out. In Italy, the number of serogroup C meningococci fell progressively following the introduction of the MenC conjugate vaccine, recommended by the Italian Ministry of Health but implemented according to different regional strategies.MethodsIMD cases from January 2005 through July 2008 reported to the National Meningococcal Surveillance System were considered for this study. Serogrouping and sero/subtyping were performed on 179 serogroup C strains received at the National Reference Laboratory of the Istituto Superiore di Sanità. Antibiotic susceptibility testing was possible for 157 isolates. MLST (Multilocus sequence typing), porA VRs (Variable Region) typing, PFGE (Pulsed Field Gel Electrophoresis), VNTR (Variable Number Tandem Repeats) analyses were performed on all C:2a and C:2b meningococci (n = 147), following standard procedures.ResultsIn 2005 and 2008, IMD showed an incidence of 0.5 and 0.3 per 100,000 inhabitants, respectively. While the incidence due to serogroup B remained stable, IMD incidence due to serogroup C has decreased since 2006. In particular, the decrease was significant among infants. C:2a and C:2b were the main serotypes, all C:2a strains belonged to ST-11 clonal complex and all C:2b to ST-8/A4. Clinical manifestations and outcome of infections underlined more severe disease caused by C:2a isolates. Two clusters due to C:2a/ST-11 meningococci were reported in the North of Italy in December 2007 and July 2008, respectively, with a high rate of septicaemia and fatal outcome.ConclusionPublic health surveillance of serogroup C invasive meningococcal disease and microbiological/molecular characterization of the isolates requires particular attention, since the hyper-invasive ST-11 predominantly affected adolescents and young adults for whom meningococcal vaccination was not recommended in the 2005–2007 National Vaccine Plan.

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“…In particular, multilocus sequence typing (MLST) (10), porA variable region (VR) typing (5,13), and pulsed-field gel electrophoresis (PFGE) (6) were performed in parallel with a sample of 25 serogroup C strains from our reference laboratory collection (13 representatives of serotype C:2b and 12 representatives of C:2a, with the different serosubtypes P1.5, P1.5,2, and P1.2). All isolates were serogrouped and serosubtyped as previously described (12).…”

mentioning

confidence: 99%

First Report of Capsule Replacement among Electrophoretic Type 37 Neisseria meningitidis Strains in Italy

et al. 2003

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This report describes the C-to-B capsular switching in four Neisseria meningitidis strains belonging to the electrophoretic type 37 (ET-37) complex. In particular, one strain belonged to the new sequence type 1860, which was first detected in the year 2000 in Italy and is now frequently isolated. The presence of switched serogroup B strains deserves special attention if they prove as able to spread as their serogroup C progenitors belonging to the hypervirulent ET-37 complex.

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Typing of sequential bacterial isolates by pulsed-field gel electrophoresis

Cited by 18 publications

References 15 publications

Neisseria meningitidisSerogroup X Sequence Type 2888, Italy

Neisseria meningitidisSerogroup X Sequence Type 2888, Italy

Serogroup C meningococci in Italy in the era of conjugate menC vaccination

First Report of Capsule Replacement among Electrophoretic Type 37 Neisseria meningitidis Strains in Italy

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