Tyrosinase Inhibitory and Anti-oxidative Effects of Lactic Acid Bacteria Isolated from Dairy Cow Feces

Abstract

Overproduction and accumulation of melanin cause a number of skin diseases. The inhibitors of tyrosinase are important for the treatment of skin diseases associated with hyper-pigmentation after UV exposure and application in cosmetics for whitening and depigmentation. Reactive oxygen species (ROS) including hydrogen peroxide are generated by chemical substances and metabolic intermediates and cause various diseases including cancer and heart diseases. We have isolated four different lactic acid bacteria (LAB) strains from dairy cow feces and investigated the tyrosinase inhibition and anti-oxidative effects of culture filtrates prepared from the isolated bacteria, which are designated as EA3, EB2, PC2, and PD3. To investigate optimal culture conditions isolated LAB strains, the measurements of tyrosinase inhibitory and anti-oxidative activities were performed. The results of tyrosinase inhibitory activities revealed that Enterococcus sp. EA3 showed about 65% at culture conditions (14 h, 30 °C, pH 8, and 0% NaCl), Enterococcus sp. EB2 about 65% at culture conditions (12 h, 30 °C, pH 9, and 0% NaCl), Pediococcus sp. PC2 about 80% at culture conditions (20 h, 30 °C, pH 6, and 0% NaCl), and Pediococcus sp. PD3 about 80% at culture conditions (20 h, 30 °C, pH 8, and 0% NaCl), respectively. In addition, anti-oxidative activities against four different LAB strains showed approximately more than 30% at optimal conditions for the measurements of tyrosinase inhibitory activities. From the results, we have suggested that the isolated four LAB strains could be useful for a potential agent for developing anti-oxidants and tyrosinase inhibitors.

A comprehensive review on tyrosinase inhibitors

“…Pp2-459 176 and a toxic strain of the cyanobacterium, Oscillatoria agardhii 177 . Interestingly, some probiotics such as Lactobacillus sp 178 . which are used in the fermentation process have been investigated as natural tyrosinase inhibitor sources.…”

Mechanisms of Action and Preservation Effects of Packaging Systems for Mushrooms: Novel Approaches to Preserve Irish Edible Mushrooms

“…The mechanisms of action of packaging systems can be explained by enzymatic, (such as tyrosinase and polyphenol oxidase ac- A smaller class of alkaloids and polyphenols found in fungi, including Aspergillus sp., Paecilomyces sp., Trichoderma sp., Phellinus linteus, Daedalea dickinsii, and Dictyophora indusiata, have been reported to selectively block the enzyme and are a source of novel tyrosinase inhibitors [40,41]. Studies have shown that four distinct strains of lactic acid bacteria isolated from cow faeces exhibit tyrosinase inhibitory activity [80]. For instance, the most active compounds within the group of natural flavones, flavanols, isoflavones, and flavanones inhibited mushroom tyrosinase with an IC 50 of 44-500 µM, while natural anthocyanidins, aurones, and chalcones had an IC 50 ranging from 18 to 106.7 µM, which was in comparison to kojic acid (a potent inhibitor of tyrosinase) with a tyrosinase inhibitory activity of IC 50 of 59-318 µM (Table 3).…”

A Study on the Tyrosinase Inhibitory and Antioxidant Effect of Microalgae Extracts

“…Melanin is the final product produced by melanogenesis and is influenced by various factors such as hormonal changes and nutritional status. Besides, melanogenesis can cause damage to cell membranes and DNA by generating free radicals, causing DNA lipid peroxidation, reducing membrane fluidity, and DNA mutations leading to cancer and degenerative diseases [4,5]. This melanogenesis occurs in melanocytes located in the epidermis basement layer, and tyrosinase acts as the main enzyme in this process.…”