Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment

Kamagaju, Léocadie; Morandini, Renato; Bizuru, Elias; Nyetera, Polycarpe; Nduwayezu, Jean Baptiste; Stévigny, Caroline; Ghanem, Ghanem; Duez, Pierre

doi:10.1016/j.jep.2013.02.010

Cited by 45 publications

(31 citation statements)

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“…Natural extracts with the ability to scavenge free radicals are a promising source for novel skin protection, whitening and anti-aging cosmeceuticals (Hamed et al, 2015;Saewan and Jimtaisong, 2015;Wang et al, 2015). In addition, tyrosinase as well as elastase inhibitors are known to be used in various skin related treatments as well as in cosmetics as antiwrinkle and skin-whitening agents (Kim et al, 2012;Kamagaju et al, 2013). On the other hand, elastase enzymatically breaks down elastins fibers, that together with collagen, are responsible for the mechanical properties of connective tissue (Antonicelli et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Tyrosinase is the rate-limiting enzyme for controlling the production of melanin, involved in determining the color of mammalian skin and hair (Kim and Uyama, 2005;Kim et al, 2012;Kamagaju et al, 2013). Inhibiting tyrosinase activity has been targeted for the prevention of conditions related to the hyperpigmentation of the skin, such as melasma and age spots (Ando et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

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Nowadays, there is huge interest in natural products obtained from marine organisms that can promote a state of health and well-being for humans. Microalgae represent a primary source of bioactive compounds that could be used as functional ingredients in cosmetic formulations. The aim of the present study is to evaluate, for the first time, the effects of Nannochloropsis gaditana extract against oxidative stress in human primary fibroblasts so as to investigate the potential applications of it in cosmetics. To gain an insight into the molecular mechanisms of N. gaditana bioactivity, we developed a new RT-qPCR platform for studying transcript accumulation for an array of selected genes (up to 100) involved in many skin-related processes including anti-aging, hydration, oxidative stress response, and DNA damage. For the oxidative stress evaluation, H 2 O 2 was used as a stressor. The study of the transcript accumulation of genes revealed that N. gaditana extract exhibits skin protection properties by mediating oxidative responses and apoptosis (including SOD1, GPX1, BID), positively regulates genes involves in skin texture and hydration (including AQP3, Col6A1, FBN1) and modulates the expression of genes involved in skin irritation, DNA damage and aging (including IL1R, PCNA, FOXO3). These findings indicate that the specific N. gaditana extract possesses significant in vitro skin protection activity against induced oxidative stress, and provide new insights into the beneficial role of microalgae bioactive compounds in cosmetic formulations protecting skin from oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

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“…Tyrosinase inhibition was determined as described (Tomita et al, 1990) with minor modifications (Kamagaju et al, 2013). In 96-well plates, 80 L of base 2 logarithmic dilutions of a fresh solution of tested extracts (50, 100 and 500 g/mL) or reference compound (kojic acid, 142 and 1421 g/mL) were mixed with 80 L of 4 mM l-dopa -7 mM l-tyrosine solution [aq.]…”

Section: Measurement Of Tyrosinase Inhibitionmentioning

confidence: 99%

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Brahmi

Hauchard

Guendouze

et al. 2015

Industrial Crops and Products

131

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International audienceThe leaves of three Mentha species harvested in Algeria, Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR) were examined for their content in polyphenols and for some activities-linked biological properties these could impart. The contents in total phenolics (TPC) and flavonoids (TFC) were evaluated by the Folin–Ciocalteu and the aluminum chloride methods, respectively. Whereas MS showed the highest TPC (12.0 ± 0.3 mg gallic acid equivalents/g of dry weight), MR had the highest content in TFC (3.3 ± 0.1 mg quercetin equivalents of dry weight). The pharmacological properties of these extracts were evaluated by assessing in vitro their antioxidant and antityrosinase activities. The modulation of mushroom tyrosinase activity was measured by colorimetry of the melanins formed in the presence of tyrosine. MS exhibited the strongest radical scavenging activity (RSA) in all assays: (i) the IC50s values to neutralize the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS+) and the 2,2′-diphenyl-1-picrylhydrazyl radicals (DPPH) were 10.3 ± 0.9 and 16.2 ± 0.2 μg/mL, respectively; and (ii) its original electrochemically measured superoxide quenching index value is 188 ± 37 μg/mL (AI50). MR however showed the highest tyrosinase inhibitory activity (IC50 = 108 ± 20 μg/mL). A silica gel thin-layer chromatography (TLC) technique revealed the presence of caffeic and rosmarinic acids and diosmin in all extracts. These results were confirmed by high performance liquid chromatography with diode array detection (HPLC/DAD

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“…A better contrast can also be obtained with slight changes in the substrate. Using the TLC bioassay with a mixture of l-DOPA and l-tyrosine as a substrate, several tyrosinase inhibitors could be clearly detected from some other African plants traditionally used for skin lightening (Kamagaju et al, 2013). Interestingly, some compounds led to an activation of tyrosinase, noticeably as dark-brown spots on the plate, and were further confirmed by an increased melanogenesis in melanoma human cell lines (Figure 11).…”

Section: Tyrosinase Inhibitionmentioning

confidence: 96%

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

Urbain

Simões-Pires

2014

Encyclopedia of Analytical Chemistry

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Thin‐layer chromatography ( TLC ) is a widespread separation technique in the field of plant analysis and offers multiple advantages. This versatile technique can provide useful information about the qualitative or semiquantitative chemical composition of a mixture, thanks to coupling, with the detection and spectroscopic techniques and devices for image treatment. Moreover, it can contribute to the detection of biological activity by combining chromatography with bioassays, such as antimicrobial or enzyme inhibition tests. This procedure, providing simultaneous chromatographic separation and in situ assessment of a given biological activity, is named TLC bioautography. The major advantage of this technique is the ability to locate active substances from a complex mixture without prior sample purification. This avoids time‐consuming and tedious isolation of already‐known or inactive compounds. As this method is simple and cost effective, it is suitable for preliminary rapid screening to detect potential activity, as well as being a useful monitoring tool to track the bioguided isolation of active components. In this chapter, we focus on up‐to‐date TLC bioautographic assays, detail their mechanisms and applications in the field of natural drug discovery, and discuss the recent advances of this method that might appear at first glance to be a trivial approach.

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Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment

Cited by 45 publications

References 46 publications

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

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