U three protein 14a (UTP14A) promotes tumour proliferation and metastasis via the PERK/eIF2a/GRP78 signalling pathway in oesophageal squamous cell carcinoma

Abstract

Metastasis and malignant proliferation are major obstacles to the treatment of oesophageal squamous cell carcinoma (ESCC), and UTP14A is associated with poor prognosis in ESCC. However, its mechanisms have not been fully elucidated. The TCGA and GEO databases were used to identify candidate target genes and possible downstream targets. Then, the effects were determined in vitro and in vivo through knockdown and overexpression techniques, and the mechanism was explored. UTP14A was significantly higher in the tumour tissue of ESCC patients than in normal tissue. Knockdown of UTP14A significantly suppressed the migration and proliferation of ESCC cells. The PERK/eIF2a signalling pathway was positively regulated by UTP14A, and its tumour-promoting effect was further activated by overexpression of UTP14A. In conclusion, UTP14A might promote the proliferation and metastasis of ESCC cells by inducing PERK/eIF2a signalling pathway expression.

Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma

“…UTP14A (U three protein 14A) plays a key role in the synthesis of ribosomes and 18S rRNA; however, in the last few years, data have appeared on the association of this gene with some types of tumors—hepatocellular carcinoma, colorectal carcinoma, and esophageal squamous cell carcinoma [ 74 ]. The exact mechanisms of its activity are unknown, but we have shown for the first time that the expression of this gene correlates with genes involved in the development of HNSCC.…”

MicroRNA-Based Markers of Oral Tongue Squamous Cell Carcinoma and Buccal Squamous Cell Carcinoma: A Systems Biology Approach

“…Schatz et al [66] found that it signifcantly overexpressed EIF2S1, EIF5A, and EIF6 when compared to healthy tissue samples. Recently, Li et al [67] linked the UTP14A overexpression in oesophageal squamous cell carcinoma (ESCC) cells to the upregulation of PERK/eIF2a signaling pathway, leading to the cell cycle process and migration of ESCC cells. Te overexpression of OTSCC, oral tongue squamous cell carcinoma; BSCC, buccal squamous cell carcinoma; GO, Gene Ontology.…”

UTP14A, DKC1, DDX10, PinX1, and ESF1 Modulate Cardiac Angiogenesis Leading to Obesity-Induced Cardiac Injury

“…It has been noted that UTP14A is involved in the development of colorectal cancer by promoting angiogenesis, while the inhibition of UTP14A can improve the prognosis of patients [ 30 , 31 ]. The upregulation of UTP14A was associated with the progression of esophageal cancer [ 32 ]. Similarly, PIN2/TRF1-interacting telomerase inhibitor 1 (PinX1) is involved in the development of cancer through angiogenesis [ 33 ].…”