UBE2C cell-free RNA in urine can discriminate between bladder cancer and hematuria

Abstract

BackgroundThere is growing interest in circulating nucleic acids as cancer detection biomarkers. Therefore, the aim of the present study was to identify a key urinary cell-free RNA marker that may assist in the diagnosis of BC.ResultsFive cell-free RNAs were selected as candidate cell-free RNAs from tissue microarray data. An area under the curve (AUC) cut-off value of 0.7 in receiver operating characteristic (ROC) curve analysis identified four urinary cell-free RNAs for further analysis (CDC20, ESM1, UBE2C, and CA9; AUC = 0.716, 0.704, 0.721, and 0.702, respectively). Binary logistic regression analysis revealed that high expression of UBE2C was significantly associated with BC (OR, 1.754; CI, 1.147–2.682; p = 0.010). Analysis of UBE2C expression in urine samples from BC patients and hematuria controls yielded an AUC of 0.839, with a sensitivity of 82.5% and a specificity of 76.2%. UBE2C levels was significantly increased in G2 and G3 tumors compared to normal controls (p <0.001, respectively).Materials and MethodsUrine samples from 212 BC patients and 106 normal controls (64 healthy individuals and 42 with hematuria) were examined. The candidate cell-free RNAs identified from tissue microarrays derived from BC and normal control tissues was then measured in the urine samples.ConclusionsThe levels of urinary UBE2C cell-free RNA were significantly higher in BC samples than in normal and hematuria control samples. The higher levels of urinary UBE2C cell-free RNA in BC might reflect high expression in BC tissues. Therefore, urinary UBE2C cell-free RNA may be a valuable diagnostic marker for BC.

UbcH10 a Major Actor in Cancerogenesis and a Potential Tool for Diagnosis and Therapy

“…The UbcH10 overexpression could be investigated directly on CTCs by immunocytochemical approaches, as demonstrated for other biomarkers [66][67][68][69], or by searching for its transcript in cell free circulating RNA in biological fluids. As it has been shown by recent, encouraging data from a study carried out on the urine of subjects with breast cancer [70], the analysis of UBE2C transcript levels could become a sensitive marker that can be exploited in diagnostics. More studies should be designed and performed in the near future to test the real power of what could be an exceptional tool for diagnostics and clinical practice.…”

Identification of Key Biomarkers in Bladder Cancer: Evidence from a Bioinformatics Analysis

“…UBE2C is involved in the regulation of mitotic cyclins, cell cycle progression, and cancer progression [77]. Kim et al demonstrated that UBE2C in the urine of BCa patients is significantly higher than in normal urine, and suggested it as a prognostic biomarker for BCa [78].…”

UBE2C Overexpression Aggravates Patient Outcome by Promoting Estrogen-Dependent/Independent Cell Proliferation in Early Hormone Receptor-Positive and HER2-Negative Breast Cancer

“…Dysregulation of ubiquitin-dependent processes is involved in the occurrence and progression of cancer, and the ubiquitin-proteasome system has thus become a new druggable target in certain cancers such as bladder carcinoma, cervical cancer, colorectal carcinoma, esophageal squamous Abbreviations: ER, Estrogen receptor; PR, Progesterone receptor; HER2, Human epidermal growth factor receptor 2; FFPE, Formalin-fixed paraffinembedded; DMFS, Distant metastasis-free survival; DFS, Disease-free survival; OS, Overall survival. cell carcinoma, lung cancer, and pancreatic carcinoma (14)(15)(16)(17)(18)(19)(20). High UBE2C expression is associated with a high degree of malignancy, low differentiation, high metastatic tendency, and poor patient survival in a wide range of solid tumors including breast cancer (21)(22)(23)(24)(25).…”