Über die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natürlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfophosphovanillin-Reaktion

Zöllner, N.; Kirsch, Katharina

doi:10.1007/bf02045455

Cited by 777 publications

(198 citation statements)

References 12 publications

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“…Presumably, the lipid composition changed during final maturation to compounds which were less specifically detectable by the sulphophosphovanillin reaction, e.g. saturated fatty acids (Zöllner & Kirsch, 1962). Parallel to lipid deposition, the amount of cell water might have increased and kept an elevated liver weight and thus a high HSI.…”

Section: Discussionmentioning

confidence: 99%

“…We measured total lipids with the sulphophospho-vanillin method according to Zöllner & Kirsch (1962). This method is well suited for total lipid determination, comparable with gravimetric methods (Barnes & Blackstock, 1973), and allows the exact measurement of lipids in mg-quantities of tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Total lipids were determined by the sulphophospho-vanillin method according to Zöllner & Kirsch (1962). A commercial test kit (Merck, 3321) was adapted for the measurement in microplates.…”

Section: Biochemical Investigationsmentioning

confidence: 99%

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Annual changes in the nutritive state of North Sea dab

Saborowski¹,

Búchholz²

1996

Journal of Fish Biology

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The nutritive state of dab Limanda limanda was investigated over a 2-year period at a fixed sampling site northwest of Helgoland (German Bight, North Sea), with respect to feeding habits and the accumulation of biochemical storage products. Ophiuroids formed the main weight of food organisms (50%) while polychaetes (10%), molluscs and crustaceans (<5% each) were less frequent. Feeding activity in males varied between summer and winter, while females fed more constantly. The condition factor and the hepatosomatic index showed characteristic seasonal cycles in both sexes. The glycogen content in the liver reached 40-60 mg g 1 FW in summer and fell to about 10-20 mg g 1 FW in late winter. Total lipids of the liver showed a distinct seasonal cycle with 400 mg g 1 FW in summer and a minimum of 50-100 mg g 1 FW in spring. The lipid content of the muscle ranged from 5 to 6 mg g 1 FW and did not vary significantly between seasons.1996 The Fisheries Society of the British Isles

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Annual changes in the nutritive state of North Sea dab

Saborowski¹,

Búchholz²

1996

Journal of Fish Biology

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“…for measurements of the lipid and protein contents. The lipid content of the homogenate was determined with the sulphophosphovanillin method (Zöllner and Kirsch 1962) modified for microplates (Torres et al 2007b). The protein content of the homogenate was determined using a BioRad (München, Germany) DC Protein Assay kit (based on the method of Lowry et al 1951), which was also modified for microplates (Torres et al 2007a).…”

Section: Measurements Of Body Size Dry Mass and Elemental Compositionmentioning

confidence: 99%

Adaptive traits in ecology, reproduction and early life history of Sesarma meridies, an endemic stream crab from Jamaica

Anger

Torres

Nettelmann

2007

Mar. Freshwater Res.

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The endemic Jamaican freshwater crab Sesarma meridies lives in shady microhabitats on river banks, where temperature and pH are lower and ion concentrations higher than in mid-stream water. Ovigerous females were found to release up to 140 yolk-rich larvae (hatching period: 1 week; >90% at night). Larval development comprised two fully lecithotrophic zoeal stages and a feeding megalopa, which was also able to develop without food (facultative lecithotrophy). After metamorphosis in complete absence of food, juvenile crabs showed reduced body size, delayed moulting, and enhanced mortality. Endotrophic development was fuelled by internal lipid reserves; proteins were conserved as structural components of tissues and organs. Fed megalopae enhanced their protein content rather than re-stocking previously lost lipid reserves. Ecdysial biomass (CHN) losses were very low (zoeal stages: 1–2%; megalopa: 3–7%), showing an energy-saving production of thin exuviae. An extended hatching period may reduce intraspecific competition or cannibalism among juveniles; nocturnal hatching should reduce the predation on larvae. Large egg size, enhanced yolk reserves, an extended embryonic development (7 weeks at 24°C; implying a prolonged period of brood care), abbreviated and partially food-independent larval development, and reduced exuvial losses are considered as life-history adaptations to unpredictable planktonic food availability in the breeding habitat.

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“…The concentrations in erythrocytes obtained from HPLC were corrected by hematocrit values in each sample. Total lipid concentration was measured by a colorimetric method (Diagnos, France) (Zoellner & Kirsch, 1962). Plasma and erythrocyte fatty acids were directly transesteri®ed without previous lipid extaction, by the method proposed by Rodriguez-Palmero & Lo Âpez (Rodriguez-Palmero et al, 1997b).…”

Section: Blood Sampling and Analysismentioning

confidence: 99%

Plasma and erythrocyte α-tocopherol and plasma retinol concentrations in term infants fed formula enriched with long-chain polyunsaturated fatty acids

et al. 1998

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Objective: To assess the effect of long-chain polyunsaturated fatty acids (LCPUFA)-and vitamin Esupplemented formula feeding on erythrocyte and plasma a-tocopherol (VE), and plasma retinol (VA) concentrations in neonates and to compare these values with those found in infants feeding on infant formula without LCPUFA or breast milk Setting: University Hospital of Granada, Spain. Subjects: 49 full-term infants. Design and intervention: Subjects who chose not to breast feed were fed either (i) unsupplemented infant formula (F) or (ii) infant formula supplemented with LCPUFA and vitamin E (FL). a-Tocopherol and retinol were measured at 7 days, 1 month and 3 months. Results: Plasma and erythrocyte VE concentrations and plasma VE/total lipids ratio increased signi®cantly in all groups at 1 month of life (P`0.05), but did not change signi®cantly between 1 month and 3 months in any group (P b 0.05). Erythrocyte VE and VA retinol concentrations were higher in infants fed an infant formula than in breast milk-fed infants at 1 month of life (P`0.05). Finally, there were no signi®cant differences in plasma or erythrocyte VE levels, plasma VA or plasma VE/total lipid ratio between any groups at 3 months of life (P b 0.05). Conclusion: Infants fed on LCPUFA-and vitamin E-supplemented infant formula for 3 months have similar vitamin E and A status to infants fed on breast milk or infant formula without LCPUFA supplementation.

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Über die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natürlichen Lipoiden (allen bekannten Plasmalipoiden) gemeinsamen Sulfophosphovanillin-Reaktion

Cited by 777 publications

References 12 publications

Annual changes in the nutritive state of North Sea dab

Annual changes in the nutritive state of North Sea dab

Adaptive traits in ecology, reproduction and early life history of Sesarma meridies, an endemic stream crab from Jamaica

Plasma and erythrocyte α-tocopherol and plasma retinol concentrations in term infants fed formula enriched with long-chain polyunsaturated fatty acids

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