Ubiquitous transgene expression and Cre-based recombination driven by the <i>ubiquitin</i> promoter in zebrafish

Mosimann, Christian; Kaufman, Charles K.; Li, Pulin; Pugach, Emily K.; Tamplin, Owen J.; Zon, Leonard I.

doi:10.1242/dev.059345

Cited by 425 publications

(555 citation statements)

References 31 publications

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“…The cmlc2:CreER transgenic was obtained from the Poss laboratory (Duke University, Durham, NC, USA) (Kikuchi et al, 2010). The ubi:CreER transgenic was obtained from the Zon laboratory (Boston Children's Hospital, Boston, MA, USA) (Mosimann et al, 2011). The s1102t Gal4 driver line was obtained from the Zebrafish International Resource Center (Eugene, OR, USA).…”

Section: Zebrafish Husbandry and Strainsmentioning

confidence: 99%

“…The ubi:Zebrabow transgenic construct was generated by inserting the ubiquitin b promoter (Mosimann et al, 2011) ) line (referred to as ubi:Zebrabow-M or line M) had higher expression than ubi:Zebrabow-S. Using quantitative PCR of genomic DNA with ubi:Zebrabow-S heterozygote as a single copy control, 16-32 copies of ubi:Zebrabow transgene were found in this line.…”

Section: Ubi:zebrabow-s and Ubi:zebrabow-mmentioning

confidence: 99%

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Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

et al. 2013

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SUMMARYAdvances in imaging and cell-labeling techniques have greatly enhanced our understanding of developmental and neurobiological processes. Among vertebrates, zebrafish is uniquely suited for in vivo imaging owing to its small size and optical translucency. However, distinguishing and following cells over extended time periods remains difficult. Previous studies have demonstrated that Cre recombinase-mediated recombination can lead to combinatorial expression of spectrally distinct fluorescent proteins (RFP, YFP and CFP) in neighboring cells, creating a 'Brainbow' of colors. The random combination of fluorescent proteins provides a way to distinguish adjacent cells, visualize cellular interactions and perform lineage analyses. Here, we describe Zebrabow (Zebrafish Brainbow) tools for in vivo multicolor imaging in zebrafish. First, we show that the broadly expressed ubi:Zebrabow line provides diverse color profiles that can be optimized by modulating Cre activity. Second, we find that colors are inherited equally among daughter cells and remain stable throughout embryonic and larval stages. Third, we show that UAS:Zebrabow lines can be used in combination with Gal4 to generate broad or tissue-specific expression patterns and facilitate tracing of axonal processes. Fourth, we demonstrate that Zebrabow can be used for long-term lineage analysis. Using the cornea as a model system, we provide evidence that embryonic corneal epithelial clones are replaced by large, wedge-shaped clones formed by centripetal expansion of cells from the peripheral cornea. The Zebrabow tool set presented here provides a resource for next-generation color-based anatomical and lineage analyses in zebrafish.

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Section: Zebrafish Husbandry and Strainsmentioning

confidence: 99%

Section: Ubi:zebrabow-s and Ubi:zebrabow-mmentioning

confidence: 99%

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

et al. 2013

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“…To verify this transgenic line we crossed it with the ubi:loxP-EGFP-loxP-RFP line. 23 Addition of 4-hydroxytamoxifen (4-OHT) at 4 dpf showed a robust expression of RFP in pronephric tubules, while addition of the vehicle, ethyl alcohol (EtOH), resulted in no expression of RFP ( Figure 6B). The excision efficiency of the cdh17:Cre-ER T2 transgenic zebrafish line was high in each segment of the tubule (Supplemental Figure 5).…”

Section: Cre/loxp Mediated Conditional Expression Of Kim-1 In the Promentioning

confidence: 99%

Mammalian Target of Rapamycin Mediates Kidney Injury Molecule 1-Dependent Tubule Injury in a Surrogate Model

Yin¹,

Naini²,

Chen³

et al. 2015

JASN

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Kidney injury molecule 1 (KIM-1), an epithelial phagocytic receptor, is markedly upregulated in the proximal tubule in various forms of acute and chronic kidney injury in humans and many other species. Whereas acute expression of KIM-1 has adaptive anti-inflammatory effects, chronic expression may be maladaptive in mice. Here, we characterized the zebrafish Kim family, consisting of Kim-1, Kim-3, and Kim-4. Kim-1 was markedly upregulated in kidney after gentamicin-induced injury and had conserved phagocytic activity in zebrafish. Both constitutive and tamoxifen-induced expression of Kim-1 in zebrafish kidney tubules resulted in loss of the tubule brush border, reduced GFR, pericardial edema, and increased mortality. Kim-1-induced kidney injury was associated with reduction of growth of adult fish. Kim-1 expression led to activation of the mammalian target of rapamycin (mTOR) pathway, and inhibition of this pathway with rapamycin increased survival. mTOR pathway inhibition in KIM-1-overexpressing transgenic mice also significantly ameliorated serum creatinine level, proteinuria, tubular injury, and kidney inflammation. In conclusion, persistent Kim-1 expression results in chronic kidney damage in zebrafish through a mechanism involving mTOR. This observation predicted the role of the mTOR pathway and the therapeutic efficacy of mTOR-targeted agents in KIM-1-mediated kidney injury and fibrosis in mice, demonstrating the utility of the Kim-1 renal tubule zebrafish models.

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“…In addition, the following reporter lines were used: Tg(β-actin2:loxP-STOPloxP-DsRed-express) (abbreviated to β-actin:switch) (Bertrand et al, 2010); Tg(ubi:loxP-EGFP-loxP-mCherry) (abbreviated to ubi:switch) (Mosimann et al, 2011);Tg(EF1α:loxP-DsRed2-loxP-EGFP) (abbreviated to EF1α:switch) (Hans et al, 2009);Tg(hsp70l:loxP-DsRed2-loxPnlsEGFP) (abbreviated to hs:R to nG) (Knopf et al, 2011); Tg(crestin:Gal4-UAS-GFP) (Y.-Y. Chen, PhD thesis, Eberhard Karls Universität Tübingen, 2011); and Tg(−4725sox10:Cre) (Rodrigues et al, 2012).…”

Section: Transgenic Linesmentioning

confidence: 99%

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

et al. 2013

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SUMMARYAt the protochordate-vertebrate transition, a new predatory lifestyle and increased body size coincided with the appearance of a true head. Characteristic innovations of this head are a skull protecting and accommodating a centralized nervous system, a jaw for prey capture and gills as respiratory organs. The neural crest (NC) is a major ontogenetic source for the 'new head' of vertebrates and its contribution to the cranial skeleton has been intensively studied in different model organisms. However, the role of NC in the expansion of the respiratory surface of the gills has been neglected. Here, we use genetic lineage labeling to address the contribution of NC to specific head structures, in particular to the gills of adult zebrafish. We generated a sox10:ER T2 -Cre line and labeled NC cells by inducing Cre/loxP recombination with tamoxifen at embryonic stages. In juvenile and adult fish, we identified numerous established NC derivatives and, in the cranium, we precisely defined the crest/mesoderm interface of the skull roof. We show the NC origin of the opercular bones and of multiple cell types contributing to the barbels, chemosensory organs located in the mouth region. In the gills, we observed labeled primary and secondary lamellae. Clonal analysis reveals that pillar cells, a craniate innovation that mechanically supports the filaments and forms gill-specific capillaries, have a NC origin. Our data point to a crucial role for the NC in enabling more efficient gas exchange, thus uncovering a novel, direct involvement of this embryonic tissue in the evolution of respiratory systems at the protochordate-vertebrate transition.

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Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish

Cited by 425 publications

References 31 publications

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

Mammalian Target of Rapamycin Mediates Kidney Injury Molecule 1-Dependent Tubule Injury in a Surrogate Model

Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells

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