Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana

Succinate Dehydrogenase Subunit C Contributes to Mycelial Growth and Development, Stress Response, and Virulence in the Insect Parasitic Fungus Beauveria bassiana

“…Briefly, the coding sequence of each gene was amplified with the respective primers (PL x F/PL x R) (X, BbSdhC1 and BbSdhC2) using cDNA as the template. The resultant gene fragments were fused to the 5′ terminus of green fluorescence protein gene in the vector p0380-gfp-myc-bar ( 35 ).The resultant p0380-gfp-X-bar was integrated into the WT strain via Agrobacterium -mediated transformation. Putative transformants were screened on the selection plates with phosphinothricin (200 μg/mL) and confirmed under a fluorescence microscope.…”

The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii

“…DUBs can maintain ubiquitin homeostasis, which modulates the removal and processing of ubiquitin (Kim et al, 2003 ; Suresh et al, 2020 ; Snyder and Silva, 2021 ). Recent studies have demonstrated that the ubiquitin system and ubiquitin-related genes play important roles in the development and virulence of fungal pathogens (McCafferty and Talbot, 1998 ; Oh et al, 2012 ; Wang et al, 2019b , 2020 ; Wang D. Y. et al, 2021 ). However, little is known about the deubiquitination process.…”

“…Metarhizium robertsii is an insect pathogenic fungus that is widely used for the biological control of diverse insect pests (Wang and Wang, 2017 ) and has become a model fungus for studying insect-fungal pathogen interactions in fungal insect pathogens (Guo et al, 2017 ). Previously, functional analysis of some ubiquitin-related genes has been performed in entomopathogenic fungi (Wang et al, 2019b ; Wang D. Y. et al, 2021 ). In this study, we investigated the biological role of MrUbp14 (MAA_07752) in M. robertsii .…”

Essential Role of COP9 Signalosome Subunit 5 (Csn5) in Insect Pathogenicity and Asexual Development of Beauveria bassiana

“…This can be distinguished from all analysed USP genes ( uspA – G ) upregulated in A. nidulans ∆ csnE [ 37 ]. Previously, free ubiquitin accumulation was abolished through the deletion of ubi4 , an E1 gene unlike ubi1/2 or ubi3 required for cell viability [ 7 ], and increased through the deletion of E3 ubr1 (i.e., ubl7 in Figure S3B ) in B. bassiana [ 8 ], as seen in A. nidulans ∆ uspA [ 40 ]. Repressed ubi3 and upregulated ubi4 imply that their effects on ubiquitination could be counteracted as a result of csn5 disruption.…”

“…The biological control potential of such fungi involves the overall output of various cellular processes that are determinant to the virulence, stress tolerance, and conidiation capacity vital for survival/dispersal in host habitats and are controlled or affected by numerous effectors and signalling proteins [ 3 , 4 , 5 , 6 ]. The ubiquitination of target proteins is one of the posttranslational modification (PTM) mechanisms underlying the overall output [ 7 , 8 ] and is regulated by the ubiquitin–proteasome system (UPS), which comprises ubiquitin-activating (E1), -conjugating (E2) and -ligating (E3) enzymes and the ubiquitin-specific proteases (USPs) or deubiquitinases required for protein quality control and cell growth, division, differentiation, and development [ 9 , 10 , 11 ].…”