UCHL1 expression and localization on testicular development and spermatogenesis of Chinese giant salamanders

Abstract

Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73.99 %). Before the gonads of male CGSs matured, the peak level of UCHL1 expression in testes appeared in 3-year-old CGSs according to RT-qPCR and western blot. In adult testes, the level of UCHL1 protein was lower in the breeding period than in the post-breeding period, whereas the level of UCHL1 protein in interstitial fluid of adult CGS testes was higher during the breeding period than during the post-breeding period. In testicular seminiferous lobules in the developmental stage of CGSs, immunohistochemistry displayed three kinds of localizing patterns of UCHL1, including nuclear localization at half year old, cytoplasmic localization from one year to three years old, and extracellular localization in adult. In testicular seminiferous lobules of adult CGS, the different developmental germ cells were separated by cysts containing UCHL1 protein, but UCHL1 did not localize on the mature sperm. The results showed that extracellular UCHL1 loaded on exosomes, as a component of the homogeneous germ cell cysts, could regulate the synchronous development of sperm in testes of adult CGS.

The Transcriptional Cell Atlas of Testis Development in Sheep at Pre-Sexual Maturity

“…Notably, the UCHL1 gene is expressed in rodent spermatogonia and Sertoli cells, whereas in other mammals, including humans, non-primates, and domestic animals, it is expressed only in spermatocytes and the spermatogonia of seminiferous tubules [39]. UCHL1 is considered the most appropriate marker of spermatogonia and meiosis in male germ cells, consistent with the result of the present study.…”

“…Five potential genes, including UCHL1, DDX4, SOHLH1, KITLG, and PCNA, were identified, displaying different levels of their expressions during the process of spermatogenesis. For example, UCHL1 (PGP9.5), a deubiquitinating enzyme, inhibits protein degradation by reversing ubiquitin modifications [39]. Differences in the localization of UCHL1 play important roles in regulating cell proliferation and differentiation through the post-translational ubiquitination of proteins [39].…”

“…For example, UCHL1 (PGP9.5), a deubiquitinating enzyme, inhibits protein degradation by reversing ubiquitin modifications [39]. Differences in the localization of UCHL1 play important roles in regulating cell proliferation and differentiation through the post-translational ubiquitination of proteins [39]. Localization of UCHL1 in the cytoplasm is a marker of non-proliferative spermatogonia in primates, and its conservative expression regulates spermatogenesis in mammalian testes [40].…”

Remodeling of Blood Vessels by Binge Alcohol Intake in the Mouse Trigeminal Ganglia

“…We utilized two antibodies; one of them is for a synaptic and neuron-specific protein Homer1, which is a scaffold protein associated with the plasma membrane, and the other one is for a pan-neuronal marker PGP9.5, also known as UCHL1, which mainly localizes in the cytosol. 32,33) The anti-Homer1 and PGP9.5 antibodies reacted with neuronal cell bodies in the trigeminal ganglia and did not show any change in the staining pattern by ethanol administration (Fig. 5A-D).…”

Analysis of the autophagy gene expression profile of pancreatic cancer based on autophagy-related protein microtubule-associated protein 1A/1B-light chain 3

“…Previous studies have shown that the UCH family plays different roles in the progression of different tumors[40]. Ubiquitin carboxyl terminal esterase L1, a family member of UCHs, is not only overexpressed in neural tissue[41] but is also used as a marker of nerve fibers to study PNI[42].…”