UCP3 Ablation Exacerbates High-Salt Induced Cardiac Hypertrophy and Cardiac Dysfunction

Abstract

Background/Aims: Excessive salt intake and left ventricular hypertrophy (LVH) are both critical for the development of hypertension and heart failure. The uncoupling protein 3 (UCP3) plays a cardio-protective role in early heart failure development. However, the potential role for UCP3 in salt intake and LVH is unclear. Methods: UCP3^-/- and C57BL/6 mice were placed on either a normal-salt (NS, 0.5%) or a high-salt (HS, 8%) diet for 24 weeks. The cardiac function, endurance capacity, energy expenditure, and mitochondrial functional capacity were measured in each group. Results: Elevated blood pressure was only observed in HS-fed UCP3^-/- mice. High salt induced cardiac hypertrophy and dysfunction were observed in both C57BL/6 and UCP3^-/- mice. However, the cardiac lesions were more profound in HS-fed UCP3^-/- mice. Furthermore, HS-fed UCP3^-/-mice experienced more severe mitochondrial respiratory dysfunction compared with HS-fed C57BL/6 mice, represented by the decreased volume of oxygen consumption and heat production at the whole-body level. Conclusion: UCP3 protein was involved in the incidence of high-salt induced hypertension and the progression of cardiac dysfunction in the early stages of heart failure. UCP3 ablation exacerbated high-salt-induced cardiac hypertrophy and cardiac dysfunction.

Thiopental sodium loaded solid lipid nano-particles attenuates obesity-induced cardiac dysfunction and cardiac hypertrophy via inactivation of inflammatory pathway

“…Previous research suggests that LVH enhanced cardiovascular diseases related mortality and morbidity (Lang et al., 2018 ; Wang et al., 2019 ). It is proofed that the CVD risk factors are linked with the expansion of LVH.…”

“…In the meantime, increased BP level plays a crucial role in activating LV myocardial expansion via chronic hemodynamic overload and enhanced central pressure. Primary hypertension disease in which environmental and genetic factors interact, with the fat intake being a significant environmental marker (Lang et al., 2018 ; Wang et al., 2019 ). Various studies on the animals and epidemiological surveys have exhibited a positive correlation between the BP level and fat intakes.…”

“…For the estimation of the hemodynamic index, blood samples directly from the heart and kept in the heparin pretreated tubes and centrifuged for 10 min at the 35 g rpm at the 4 °C and the supernatant was stored at the −80 °C for further analysis (Lang et al., 2018 ).…”

“…At the end of the experimental study, all group mice were sacrificed and the heart tissue were successfully removed and calculated the heart to body weight ratio (HW/BW) by dividing the heart weight (HW) via body weight (BW) after the harvest and weighting the hearts (Lang et al., 2018 ; Wang et al., 2019 ).…”

Analysis of association between common variants of uncoupling proteins genes and diabetic retinopathy in a Chinese population

“…Its mechanism is adjusting energy homeostasis though thermogenesis and regulating ROS release [28]. The association between UCPs and hypertension was discovered in lab studies [29,30], and was verified in Asian population [31,32]. Molecular epidemiology studies found UCP2 polymorphism alters the risk of developing CAD in some populations [33,34], while the relation between UCPs and obesity was widely recognized in several populations [35][36][37].…”