UHPLC-Q-Exactive Orbitrap MS/MS based untargeted lipidomics reveals fatty acids and lipids profiles in different parts of capelin (Mallotus villosus)

Yin, Mingyu; Chen, Min; Matsuoka, Ryosuke; Song, Xinyi; Xi, Yinci; Zhang, Long; Wang, Xichang

doi:10.1016/j.jfca.2022.105096

Cited by 7 publications

(2 citation statements)

References 54 publications

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“…The column temperature was set at 35 • C with an injection volume of 1 µL, and the mobile phase consisted of solvent A (0.1% formic acid and 10 mM ammonium formate in acetonitrile/water = 60:40) and solvent B (0.1% formic acid and 10 mM ammonium formate in isopropanol/acetonitrile = 90:10) at a flow rate of 0.3 mL/min. The gradient elution procedure was as follows: 0-1.5 min, 40% B; 1.5-10.5 min, 40-85% B; 10.5-14 min, 85% B; 14-14.1 min, 85-100% B; 14.1-15 min, 100% B; 15-15.2 min, 100-40% B; and 15.2-18 min, 40% B [57]. Mass spectrometry was performed on a Q Exactive™ Focus Orbitrap™ (Thermo Fisher Scientific).…”

Section: Lipomics Analysismentioning

confidence: 99%

Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds: A Potential Application in Alzheimer’s Disease?

Martens,

Zhan,

Voortman

et al. 2023

Nutrients

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The nuclear liver X receptors (LXRα/β) and peroxisome proliferator-activated receptors (PPARα/γ) are involved in the regulation of multiple biological processes, including lipid metabolism and inflammation. The activation of these receptors has been found to have neuroprotective effects, making them interesting therapeutic targets for neurodegenerative disorders such as Alzheimer’s Disease (AD). The Asian brown seaweed Sargassum fusiforme contains both LXR-activating (oxy)phytosterols and PPAR-activating fatty acids. We have previously shown that dietary supplementation with lipid extracts of Sargassum fusiforme prevents disease progression in a mouse model of AD, without inducing adverse effects associated with synthetic pan-LXR agonists. We now determined the LXRα/β- and PPARα/γ-activating capacity of lipid extracts of six European brown seaweed species (Alaria esculenta, Ascophyllum nodosum, Fucus vesiculosus, Himanthalia elongata, Saccharina latissima, and Sargassum muticum) and the Asian seaweed Sargassum fusiforme using a dual luciferase reporter assay. We analyzed the sterol and fatty acid profiles of the extracts by GC-MS and UPLC MS/MS, respectively, and determined their effects on the expression of LXR and PPAR target genes in several cell lines using quantitative PCR. All extracts were found to activate LXRs, with the Himanthalia elongata extract showing the most pronounced efficacy, comparable to Sargassum fusiforme, for LXR activation and transcriptional regulation of LXR-target genes. Extracts of Alaria esculenta, Fucus vesiculosus, and Saccharina latissima showed the highest capacity to activate PPARα, while extracts of Alaria esculenta, Ascophyllum nodosum, Fucus vesiculosus, and Sargassum muticum showed the highest capacity to activate PPARγ, comparable to Sargassum fusiforme extract. In CCF-STTG1 astrocytoma cells, all extracts induced expression of cholesterol efflux genes (ABCG1, ABCA1, and APOE) and suppressed expression of cholesterol and fatty acid synthesis genes (DHCR7, DHCR24, HMGCR and SREBF2, and SREBF1, ACACA, SCD1 and FASN, respectively). Our data show that lipophilic fractions of European brown seaweeds activate LXRs and PPARs and thereby modulate lipid metabolism. These results support the potential of brown seaweeds in the prevention and/or treatment of neurodegenerative diseases and possibly cardiometabolic and inflammatory diseases via concurrent activation of LXRs and PPARs.

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Section: Lipomics Analysismentioning

confidence: 99%

Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds: A Potential Application in Alzheimer’s Disease?

Martens,

Zhan,

Voortman

et al. 2023

Nutrients

View full text Add to dashboard Cite

show abstract

“…Ceramide non-hydroxyfatty acid-dihydrosphingosine (Cer/NDS), hexosylceramide non-hydroxyfatty acid-dihydrosphingosine (HexCer/NDS), and sphingomyelin (SM) were the most abundant lipids in the SP. As an important lipid, TAG is an intermediate for energy and lipid storage and acts as a transporter of FA in animal organisms [29]. TAG, PC, and PE are reportedly the major lipids in marine fish tissues [30].…”

Section: Identification Of Lipids During the Fermentation Of B Subtil...mentioning

confidence: 99%

Lipidomics and whole-genome sequencing identify lipid profiles in fermented golden pompano ( Trachinotus ovatus) inoculated with Bacillus subtilis: Influence of indigenous strains on lipid changes

Wu,

Wang,

Xiang

et al. 2024

Food Science and Human Wellness

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Lipidomics Analysis of Different Marine Fish Oils Using Untargeted Liquid Chromatography–Orbitrap High-Resolution Mass Spectrometry and Chemometrics

Windarsih,

Irnawati,

Suratno

et al. 2024

Chromatographia

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UHPLC-Q-Exactive Orbitrap MS/MS based untargeted lipidomics reveals fatty acids and lipids profiles in different parts of capelin (Mallotus villosus)

Cited by 7 publications

References 54 publications

Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds: A Potential Application in Alzheimer’s Disease?

Activation of Liver X Receptors and Peroxisome Proliferator-Activated Receptors by Lipid Extracts of Brown Seaweeds: A Potential Application in Alzheimer’s Disease?

Lipidomics and whole-genome sequencing identify lipid profiles in fermented golden pompano ( Trachinotus ovatus) inoculated with Bacillus subtilis: Influence of indigenous strains on lipid changes

Lipidomics Analysis of Different Marine Fish Oils Using Untargeted Liquid Chromatography–Orbitrap High-Resolution Mass Spectrometry and Chemometrics

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