Ultra-high performance liquid chromatography combined with mass spectrometry for determination of aflatoxins using dummy molecularly imprinted polymers deposited on silica-coated magnetic nanoparticles

Tan, Lei; He, Rong; Chen, Kuncai; Peng, Rongfei; Huang, Cong; Yang, Rong; Tang, Yawen

doi:10.1007/s00604-016-1790-y

Cited by 56 publications

(17 citation statements)

References 33 publications

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“…The best extraction conditions involved using 80 mg of sorbent to achieve the extraction of aflatoxins from corn samples, obtaining recovery values ranging from 75 to 99%. Tan et al also developed a magnetic dummy MIP for the determination of aflatoxins from corn and tea leaves [73]. In this case, the magnetic core was of Fe 3 O 4 which was first coated with silica and afterwards the silanol groups of the silica surface were modified with the MIP.…”

Section: Micro-magnetic Solid-phase Extraction (µ-Mspe)mentioning

confidence: 99%

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

2020

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Natural toxins are chemical substances that are not toxic to the organisms that produce them, but which can be a potential risk to human health when ingested through food. Thus, it is of high interest to develop advanced analytical methodologies to control the occurrence of these compounds in food products. However, the analysis of food samples is a challenging task because of the high complexity of these matrices, which hinders the extraction and detection of the analytes. Therefore, sample preparation is a crucial step in food analysis to achieve adequate isolation and/or preconcentration of analytes and provide suitable clean-up of matrix interferences prior to instrumental analysis. Current trends in sample preparation involve moving towards “greener” approaches by scaling down analytical operations, miniaturizing the instruments and integrating new advanced materials as sorbents. The combination of these new materials with sorbent-based microextraction technologies enables the development of high-throughput sample preparation methods, which improve conventional extraction and clean-up procedures. This review gives an overview of the most relevant analytical strategies employed for sorbent-based microextraction of natural toxins of exogenous origin from food, as well as the improvements achieved in food sample preparation by the integration of new advanced materials as sorbents in these microextraction techniques, giving some relevant examples from the last ten years. Challenges and expected future trends are also discussed.

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Section: Micro-magnetic Solid-phase Extraction (µ-Mspe)mentioning

confidence: 99%

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

2020

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“…Four milliliters (10 μg/mL) AFB1 solution (prepared in methanol-water) was added into the tubes. The mixture was vigorously shaken at 2500 rpm for a series of time (1,2,3,6,9,12,15,20,25,30,35,40, and 60 min). The tubes were centrifuged and the supernatants were measured by HPLC.…”

Section: Dynamics Of Polymersmentioning

confidence: 99%

Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample

Song

Chen

et al. 2019

J of Separation Science

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A kind of molecularly imprinted polymers modified with biocompatible medium was prepared by suspension polymerization. The obtained hybrid materials were used as the adsorbents for the solid‐phase extraction of aflatoxins B1 in real samples. A structural analog of the target, 6‐methyl‐4‐phenylchroman‐2‐one was used as the pseudo‐template, owing to their lower toxicity and cheaper price compared with aflatoxins B1; and methacrylic acid and glycidyl methacrylate were used as the co‐monomers. Scanning electron microscopy and size distribution analysis were used to characterize the obtained polymers. The extraction parameters were optimized to achieve the desired extraction performance. The polymer solid‐phase extraction coupled with high‐performance liquid chromatography was successfully applied to determine aflatoxins B1 from soy sauce without the process of protein removal. Under the optimum extraction conditions, the detection results of aflatoxins B1 in lab‐made column in soy sauce samples was carried out, with a recovery rate of 96%. The established method presented a linear range from 10 to 1000 ng/mL with the coefficient of determination of 0.9994 and the limit of detection of 0.05 ng/mL. Likewise, the inherent selectivity of lab‐made column towards aflatoxins B1, Ochratoxin A, and Zearalenone was demonstrated.

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“…Tan et al [11] discovered that the MIP they had prepared using 5,7-dimethoxycoumarin as a dummy template showed high adsorption capability for four aflatoxins (aflatoxins B1, B2, G1 and G2), and also showed that the rebinding capacity of the MIPs to the aflatoxins is much better than to three other mycotoxins. On the other hand, their NIPs did not exhibit an obvious difference in rebinding capacity between aflatoxins and other mycotoxins.…”

Section: Selectivity Of the Mipmentioning

confidence: 99%

“…Analytical techniques like gas chromatography (GC) or gas chromatography tandem mass spectrometry (GC-MS) have also traditionally been employed for aflatoxin analysis. However, HPLC-MS/ MS has become the technique of choice due its ability to rapidly analyze a more diverse set of analytes with better sensitivity [11]. These methods, however, lack direct application to food samples because the contaminants are present at very low concentrations in highly complex and morphologically structured matrices [12].…”

mentioning

confidence: 99%

“…However, several authors have developed methods for the extraction of aflatoxins from real food samples using molecularly imprinted polymers. For instance, Tan et al [11] described a method for the extraction of aflatoxins using dimethoxycoumarin (DMC) as an aflatoxin mimicking template (dummy) deposited on silica-coated magnetic nanoparticles. To surface graft imprint the functional monomers on the surface of the magnetic particles, they used vinyl functionalized superparamagnetic Fe 3 O 4 particles as the supports, 5,7-dimethoxycoumarin (DMC) as the dummy template, and methacrylic acid and 4-vinyl pyridine as the functional monomers.…”

mentioning

confidence: 99%

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Development of an aflatoxin B1 specific molecularly imprinted solid phase extraction sorbent for the selective pre-concentration of toxic aflatoxin B1 from child weaning food, Tsabana

Semong¹,

Batlokwa²

2017

Molecular Imprinting

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This paper presents the synthesis, optimization and application of a molecularly imprinted polymer (MIP) sorbent for the selective extraction and pre-concentration of the potent toxin, aflatoxin B1 (AFB1), from the child weaning food, Tsabana (manufactured in Serowe, Botswana). As a food safety regulatory measure, Tsabana must be cleared of hazardous aflatoxins, especially AFB1, before consumption. This is because AFB1 is the most common and potent of the aflatoxins commonly found in cereals. Accurate analysis of AFB1 is challenging because it exists in very low concentrations in complex, ‘dirty’ matrices such as food, making it difficult to detect using analytical instruments, even if these analytical techniques have sensitivities at the femto level. The MIP extraction sorbent synthesized in this paper deals with these challenges by selectively pre-concentrating AFB1 from real Tsabana samples, successfully achieving a pre-concentration factor of 5 and therefore significantly increasing ABF1 signal intensity for easier detection. Further advantages of this system include the short time (25.0 minutes) and reasonable optimal MIP dose (20.0 mg) needed for maximum AFB1 extraction by the sorbent. Scanning electron microscopy revealed that the prepared AFB1 powder particles have spherical geometries and reasonably small sizes (800 nm), two advantageous physical characteristics that are associated with excellent sorbent materials.

show abstract

Ultra-high performance liquid chromatography combined with mass spectrometry for determination of aflatoxins using dummy molecularly imprinted polymers deposited on silica-coated magnetic nanoparticles

Cited by 56 publications

References 33 publications

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample

Development of an aflatoxin B1 specific molecularly imprinted solid phase extraction sorbent for the selective pre-concentration of toxic aflatoxin B1 from child weaning food, Tsabana

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