Ultra high performance liquid chromatography with photodiode array detector and quadrupole time‐of‐flight tandem mass spectrometry coupled with discriminant analysis to evaluate Angelicae pubescentis radix from different regions

Abstract

A rapid and effective method was developed for the qualitative and quantitative analysis of the major chemical constituents in Angelicae pubescentis radix by ultra high performance liquid chromatography with photodiode array detection and quadrupole time-of-flight tandem mass spectrometry. The chromatographic separation was achieved on an ACQUITY UHPLC BEH C18 column (2.1 × 100 mm, 1.7 μm). Nine phenolic acids, 30 coumarins, bisabolangelone, and adenosine were identified by quadrupole time-of-flight tandem mass spectrometry. All calibration curves exhibited good linearity (r > 0.9996) within the linear ranges. The relative standard deviation calculated for intraday and interday precision, stability, and accuracy were <5%. The mean recovery ranged from 95.8 to 106%. The overall limits of detection and quantification were 0.025-0.160 and 0.100-0.560 μg/mL, respectively. Discriminant analysis was investigated as a method for evaluating the quality of the samples with 100% correction in their classification. The results demonstrated that the developed method could successfully be used to differentiate samples from different regions and could be a helpful tool for detection and confirmation of the quality of traditional Chinese medicines.

UPLC–Q‐TOF/MS characterization of efficacy substances on osteoblasts differentiation and function in rat serum after administration of Wang‐Bi tablet

“…By matching against the chemical library, 138 ingredients in WB were identified. Among of them, 14 compounds belonged to Radix Paeoniae alba (A) (Wang, Ma, et al, ), 10 compounds were from Radix Rehmanniae and Radix Rehmanniae Preparata (B) (Zhang, Song, et al, ), 11 compoundswere from Radix Angelicae pubescentis (C) (Ge et al, ) and 26 compounds were derived from Radix aconiti lateralis preparata (D) (Zhang, Song, et al, ). All of them were alkaloids.…”

Traditional Chinese Medicine of Angelicae Pubescentis Radix: A Review of Phytochemistry, Pharmacology and Pharmacokinetics

“…Bisabolangelone (80), adenosine (78), and 30 coumarins in APR were identified by UPLC-PAD-Q-TOF-MS. The developed method could successfully be used to differentiate samples from different regions and could be a helpful tool for detection and confirmation of the quality of TCM (Ge et al, 2014). However, one or several chemical constituents are not the only effective parts of APR.…”

Exploring the Therapeutic Composition and Mechanism of Jiang-Suan-Chu-Bi Recipe on Gouty Arthritis Using an Integrated Approach Based on Chemical Profile, Network Pharmacology and Experimental Support Using Molecular Cell Biology

“…The in-house library that covers all previous reported compounds from the 12 formulated herbs was established in a Microsoft office excel table, which includes compound name, molecular formula, molecular weight, chemical structures, natural source, and related references (Chen et al, 2010;Liang et al, 2013;Ge et al, 2014;Ma et al, 2014;Bai et al, 2015;Guo et al, 2015;He et al, 2016;Sun et al, 2016;Liu et al, 2018;Wang et al, 2018;Xu et al, 2018). The empirical molecular formula that matched the criterion for ppm below 10 were deduced as potential candidates by the function "Find Compounds by Formula" of Agilent MassHunter qualitative analysis software.…”