Ultra‐high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantitation of five phthalides in rat plasma: Application to a comparative pharmacokinetic study of Huo Luo Xiao Ling Dan and herb‐pair extract

Abstract

A fast, sensitive, and reliable ultra-high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous quantitation and pharmacokinetic study of five phthalides (senkyunolide A, ligustilide, butylidenephthalide, 3-butylphthalide, and levistilide A) in rat plasma after oral administration of Huo Luo Xiao Ling Dan (HLXLD) or Angelica sinensis--Ligusticum chuanxiong herb pair (DG-CX) between normal and arthritis rats. After extraction from blood, the analytes and internal standard were subjected to ultra-high performance liquid chromatography with a Shim-pack XR-ODS column (75 × 3.0 mm(2) , 2.2 μm particles) and mobile phase was composed of methanol and water (containing 0.05% formic acid) under gradient elution conditions, with an electrospray ionization source in the positive ionization and multiple reaction monitoring mode. The lower limits of quantification were 0.192-0.800 ng/mL for all the analytes. Satisfactory linearity, precision, accuracy, mean extraction recovery, and acceptable matrix effect have been achieved. The validated method was successfully applied to a comparative pharmacokinetic study of five bioactive components in rat plasma after oral administration of HLXLD or DG-CX alone, respectively, between normal and arthritic rats. The results showed that there were unlike characters of pharmacokinetics among different groups.

Pharmacokinetic parameters of three active ingredients hederacoside C, hederacoside D, and ɑ‐hederin in Hedera helix in rats

“…Moreover, double peaks were observed clearly on concentration–time profile with the second peak lower than the first. It is reported that the double peak phenomenon also exist in some other saponins such as mesaconitine, ligustilide . It is well known that drug absorption is a very complex process that manifests itself through potential interaction with a host of physicochemical and physiological variables.…”

Simultaneous determination of savaside A, acteoside, and isoacteoside in rat plasma by UHPLC–MS/MS: Comparative pharmacokinetic and bioavailability characteristics of Monochasma savatieri via different routes of administration

“…It is reported that the double peak phenomenon also exist in some compounds in nature. As drug absorption is a very complex process that manifests itself through potential interaction with a host of physicochemical and physiological variables [26][27][28]. The detected concentration of savaside A was low after oral TPG, and the parameters of savaside A could not be obtained.…”

Comparative Study on Pharmacokinetics of Four Active Compounds in Rat Plasma after Oral Administration of Raw and Wine Processed Chuanxiong Rhizoma

“…Furthermore, Q Exactive Focus Orbitrap has a fast positive and negative ion switching function, which can simultaneously detect positive and negative ions when analyzing samples. Therefore, it is a very effective way to realize simultaneously qualitative and quantitative analysis of multiple components [26][27][28][29]. In this study, full mass scan mode equipped with quick switching and dd-MS 2 modules was used, both positive and negative ions (butylidenephthalide, ligustilide and senkyunolide A were detected in positive ion mode, ferulic acid was detected in negative ions mode) were detected in one injection.…”