Ultra-sensitive high performance liquid chromatography–laser-induced fluorescence based proteomics for clinical applications

Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome

“…The system can detect proteins at sub-femtomole levels and its success stories for many clinical applications have been demonstrated. 20 Commercial HPLC systems use deuterium/xenon/mercury lamps and measure the absorption of the eluents from the HPLC column. 14,21 All these sources require relatively large power supplies and absorption measurements are much less sensitive compared to fluorescence, especially at very low concentrations, since absorption measures a very small change in a large signal, whereas fluorescence measures a small signal, where there was none before.…”

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

“…These techniques are time consuming, require prior knowledge of which antigen/antibody you are looking for, and so can be used only for specific proteins. High performance liquid chromatography-laser induced fluorescence (HPLC-LIF) technique has been used for ultrasensitive protein profiling of clinical samples like body fluids (blood, saliva, vaginal wash), tissue homogenates, and cellular lysates, for screening and early detection of oral, cervical, breast and ovarian cancer (Patil et al 2013 ; Patil et al 2015 ). Protein profiles recorded from the saliva of normal, premalignant and malignant oral cancer patients (Fig.…”

“…Protein profiles recorded from the saliva of normal, premalignant and malignant oral cancer patients (Fig. 9 ) were analysed with statistical pattern analysis methods like PCA, and discrimination of normal, pre-malignant and malignant conditions has been achieved with high specificity and sensitivity (Patil et al 2015 ). The protein profiling by HPLC-LIF has many advantages like very high sensitivity (subfemto-mole levels), no need of prior knowledge of the identity of the marker proteins for a given disease condition, and relatively fast, few tens of minutes.…”

“… 2017 [Hernández-Arteaga et al 2017 ] 94% 19 Spectrophotometry Salivary levels of zinc, iron and copper in Head and Neck Cancer 2017 [George et al 2017 ] _ 20 SERS Nasopharyngeal carcinoma NPC. SERS with PCA-LDA; non-invasive detection of NPC 2016 [Qiu et al 2016 ] 86.7% 21 SPR Recombinant human matrix metalloproteinases-9 (MMP-9); tumor progression and metastasis; saliva 2016 [Mohseni et al 2016 ] L.O.D : 8 pg/ml 22 SERS Breast cancer 2015 [Feng et al 2015 ] Malignant 74.19% 23 HPLC-LIF Various cancers 2015 [Patil et al 2015 ] L.O.D : femto-moles 24 AAS. Copper, iron, zinc and manganese in oral submucous fibrosis 2015 [Okade et al 2015 ] _ 25 Fluorescence Oral Cancer 2014 [Yuvaraj et al 2014 ] Emission spectra sensitivity 85.7% 26 Fluorescence Quantum Dots in Breast Cancer 2014 [Jokerst et al] L.O.D CEA: 0.02 ng/ml 27 HPLC-LIF Salivary protein markers; Oral cancer 2018 [Zaorska et al 2018 ] 79% 28 SPR Carbohydrate antigen 15-3 (CA15-3) in saliva 2012 [Liang et al 2012 ] L.O.D : 2.5 U/ml 29 SERS Saliva lung cancer 2011 [Li et al 2011 ] 94% 30 Optical Cancers 2008 [Tan et al 2011] L.O.D IL-8: 4 fM 31 <...>…”