Ultrabright fluorescent microsphere and its novel application for improving the sensitivity of immunochromatographic assay

Zhang, Gang-gang; Xu, Shaolan; Xiong, Yonghua; Duan, Hong; Chen, Wenyao; Li, Xiangmin; Yuan, Min; Lai, Weihua

doi:10.1016/j.bios.2019.04.023

Cited by 86 publications

(32 citation statements)

References 51 publications

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“…S3 ). Zhang et al 25 prepared solid fluorescent microspheres based on TCBPE, poly(maleic anhydride-alt-1-octadecene), poly(methyl methacrylate), and sodium dodecyl sulfate as loading fluorescent dye, functional linker, polymer framework, and surfactant, respectively, for detecting of E. coli O157:H7. The sensitivity of this method was 3.98 × 10 3 CFU/mL.…”

Section: Resultsmentioning

confidence: 99%

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

et al. 2021

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Section: Resultsmentioning

confidence: 99%

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

et al. 2021

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“…FICA is a membrane detection technique for quantitative analysis [14,15]. The double-antibody sandwich immunochromatographic assay (ICA) is generally adopted when detecting macromolecular antigens with multiple antigenic determinants (proteins, viruses, and pathogenic bacteria).…”

Section: Principle Of Quantitative Detectionmentioning

confidence: 99%

Development of a Smartphone-Based Fluorescent Immunochromatographic Assay Strip Reader

Jiang

et al. 2020

Sensors

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Fluorescence immunochromatographic assay (FICA) is a rapid immunoassay technique that has the characteristics of high precision and sensitivity. Although image FICA strip readers have the advantages of high portability and easy operation, the use of high-precision complementary metal oxide semiconductor (CMOS) image sensors leads to an increase in overall cost. Considering the popularity of CMOS image sensors in smartphones and their powerful processing functions, this work developed a smartphone-based FICA strip reader. An optical module suitable for the test strips with different fluorescent markers was designed by replacing the excitation light source and the light filter. An android smartphone was used for image acquisition and image denoising. Then, the test and control lines of the test strip image were recognized by the sliding window algorithm. Finally, the characteristic value of the strip image was calculated. A linear detection range from 10 to 5000 mIU/mL (R2 = 0.95) was obtained for human chorionic gonadotrophin with the maximum relative error less than 9.41%, and a linear detection range from 5 to 4000 pg/mL (R2 = 0.99) was obtained for aflatoxin B1, with the maximum relative error less than 12.71%. Therefore, the smartphone-based FICA strip reader had high portability, versatility, and accuracy.

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“…First, 400 µL of 0.05 M BB (pH = 8.0) in a 2-mL tube was mixed for 1 min with 100 µL of fluorescent microspheres. To activate the carboxyl groups on the surface of the microspheres (Zhang, Xu, et al, 2019;Yang et al, 2015;Zhang et al, 2016), we added 30 µL of aqueous EDC (10 mg/mL) dropwise. The solution was mixed at 37°C for 20 min and centrifuged at 13,000 rpm for 10 min at 10°C.…”

Section: Conjugation Of Fluorescent Microspheres To Mabmentioning

confidence: 99%

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

Xing

Liu

et al. 2020

Food and Agricultural Immunology

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In this study, we produced a monoclonal antibody (mAb) 2F7 against cadmium (Cd) with IC 50 of 0.20 ng/mL. The cross-reactivity of mAb 2F7 with other eight metals was < 1%. A fluorescent immunoassay strip was developed for the determination of Cd in rice. The cutoff value by visual inspection of the test strip was 50 μg/kg, and the quantitative detection range determined by the strip reader was 3.86-48.92 μg/kg. Both ic-ELISA and the fluorescent immunoassay strip can be used to detect Cd rice. Therefore, our developed fluorescent immunoassay strip may be an effective tool for detecting Cd in rice.

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Ultrabright fluorescent microsphere and its novel application for improving the sensitivity of immunochromatographic assay

Cited by 86 publications

References 51 publications

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

Development of a Smartphone-Based Fluorescent Immunochromatographic Assay Strip Reader

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

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