Ultrafast spectroscopy of UV-induced DNA-lesions — on the search for strategies which keep DNA alive

Fingerhut, Benjamin P.; Herzog, Teja T.; Ryseck, Gerald; Haiser, Karin; Graupner, Franziska F.; Heil, Korbinian; Gilch, Peter; Schreier, Wolfgang; Carell, Thomas; Vivie‐Riedle, Regina de

doi:10.1051/epjconf/20134107005

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Formation of photolesions is too fast for the triplet to be a precursor but the doorway state may be involved. [106][107] Figure 10: Schematic Jablonski diagram of the processes following UV excitation in thymine and uracil. 105…”

Section: Tautomersmentioning

confidence: 99%

How nature covers its bases

Boldissar

Vries

2018

Phys. Chem. Chem. Phys.

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The response of DNA and RNA bases to ultraviolet (UV) radiation has been receiving increasing attention for a number of important reasons: (i) the selection of the building blocks of life on an early earth may have been mediated by UV photochemistry, (ii) radiative damage of DNA depends critically on its photochemical properties, and (iii) the processes involved are quite general and play a role in more biomolecules as well as in other compounds. A growing number of groups worldwide have been studying the photochemistry of nucleobases and their derivatives. Here we focus on gas phase studies, which (i) reveal intrinsic properties distinct from effects from the molecular environment, (ii) allow for the most detailed comparison with the highest levels of computational theory, and (iii) provide isomeric selectivity. From the work so far a picture is emerging of rapid decay pathways following UV excitation. The main understanding, which is now well established, is that canonical nucleobases, when absorbing UV radiation, tend to eliminate the resulting electronic excitation by internal conversion (IC) to the electronic ground state in picoseconds or less. The availability of this rapid "safe" de-excitation pathway turns out to depend exquisitely on molecular structure. The canonical DNA and RNA bases are generally short-lived in the excited state, and thus UV protected. Many closely related compounds are longer lived, and thus more prone to other, potentially harmful, photochemical processes. It is this structure dependence that suggests a mechanism for the chemical selection of the building blocks of life on an early earth. However, the picture is far from complete and many new questions now arise.

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Section: Tautomersmentioning

confidence: 99%

How nature covers its bases

Boldissar

Vries

2018

Phys. Chem. Chem. Phys.

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“…). Short time and steady‐state spectroscopic studies have shown that depending on the local conformation of the DNA bases relative to each other in the duplex, reactions to different photoinduced products occur . It is interesting that the vast majority of UV‐induced lesions are created at pyrimidine–pyrimidine sites, indicating that the photoexcitation is somewhat concentrated at such positions.…”

Section: Structure Of Uv‐induced Dna Lesionsmentioning

confidence: 99%

Formation and Direct Repair of UV‐induced Dimeric DNA Pyrimidine Lesions

Kneuttinger

Kashiwazaki

Prill

et al. 2013

Photochem & Photobiology

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Direct repair of UV-induced DNA lesions represents an elegant method for many organisms to deal with these highly mutagenic and cytotoxic compounds. Although the participating proteins are structurally well investigated, the exact repair mechanism of the photolyase enzymes remains a vivid subject of current research. In this review, we summarize and highlight the recent contributions to this exciting field.

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Ultrafast spectroscopy of UV-induced DNA-lesions — on the search for strategies which keep DNA alive

Abstract: Abstract. UV-induced photolesions are studied in the visible and IR. While structural distortions of the DNA-backbone at the moment of light absorption are prerequisite for CPD-formation, strain from the backbone guides Dewar-formation.

Cited by 2 publications

References 7 publications

How nature covers its bases

How nature covers its bases

Formation and Direct Repair of UV‐induced Dimeric DNA Pyrimidine Lesions

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