Ultrahigh‐performance liquid chromatography coupled with triple quadrupole and time‐of‐flight mass spectrometry for the screening and identification of the main flavonoids and their metabolites in rats after oral administration of <scp><i>Cirsium japonicum</i></scp> DC. extract

Zhang, Xia; Liao, Man; Cheng, Xiaoye; Liang, Caijuan; Diao, Xingxing; Zhang, Lantong

doi:10.1002/rcm.8161

Cited by 22 publications

(12 citation statements)

References 23 publications

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“…14 In recent years, high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) has been routinely used to determine the contents of chemical compounds in traditional chemical materials and also applied in pharmacokinetic studies in rats and humans. 15,16 Moreover, it has been used to study drug metabolism, [17][18][19][20][21][22] toxicokinetics, 23,24 lipidomics, 25,26 proteomics 27,28 and metabolomics. 29,30 The main advantage of mass spectrometry (MS/MS) is the ability to detect a broad range of drugs with high sensitivity and specicity in a single analytical run.…”

Section: Introductionmentioning

confidence: 99%

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Zhang

et al. 2021

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Zhang

et al. 2021

RSC Adv.

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“…The characteristic fragment ion at m / z 505.3441, m / z 481.3888, and m / z 463.3781 signified that phosphorylation occurred at the parent nucleus. Therefore, M9 was identified as the phosphorylation metabolite of RPDQ [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetic and Metabolism Studies of 12-Riboside-Pseudoginsengenin DQ by UPLC-MS/MS and UPLC-QTOF-MSE

et al. 2018

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Pharmacokinetic and metabolism studies of 12-riboside-pseudoginsengenin DQ (RPDQ), a novel ginsenoside with an anti-cancer effect, were carried out, aiming at discussing the characteristics of the ginsenoside with glycosylation site at C-12. In the pharmacokinetic analysis, we developed and validated a method by UPLC-MS to quantify RPDQ in rat plasma. In the range of 5–1000 ng/mL, the assay was linear (R2 > 0.9966), with the LLOQ (lower limit of quantification) being 5 ng/mL. The LOD (limit of detection) was 1.5 ng/mL. The deviations of intra-day and inter-day, expressed as relative standard deviation (RSD), were ≤ 3.51% and ≤ 5.41% respectively. The accuracy, expressed as relative error (RE), was in the range –8.82~3.47% and –5.61~2.87%, respectively. The recoveries were in the range 85.66~92.90%. The method was then applied to a pharmacokinetic study in rats intragastrically administrated with 6, 12, and 24 mg/kg RPDQ. The results showed that RPDQ exhibited slow oral absorption (Tmax = 7.0 h, 7.5 h, and 7.0 h, respectively), low elimination (t1/2 = 12.59 h, 12.83 h, and 13.74 h, respectively) and poor absolute bioavailability (5.55, 5.15, and 6.08%, respectively). Moreover, the investigation of metabolites were carried out by UPLC-QTOF-MS. Thirteen metabolites of RPDQ were characterized from plasma, bile, urine, and feces of rats. Some metabolic pathways, including oxidation, acetylation, hydration, reduction, hydroxylation, glycine conjugation, sulfation, phosphorylation, glucuronidation, glutathione conjugation, and deglycosylation, were profiled. In general, both the rapid quantitative method and a good understanding of the characteristics of RPDQ in vivo were provided in this study.

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“…Further investigation suggests that pectolinarin, a flavonoid compound, may serve as the key active component in Cirsium japonicum responsible for promoting scarless regeneration. 8 Pectolinarin has garnered increasing attention due to its hemostatic, anti-inflammatory, antibacterial, antiviral and other pharmacological activities, and has been utilized in the treatment of various conditions such as diabetes and tumours. [9][10][11] Moreover, pectolinarin has shown promise in the field of tissue repair, where its antioxidant properties have been beneficial in treating liver damage.…”

Section: Introductionmentioning

confidence: 99%

Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

Chen,

Song,

Song

et al. 2024

J Cellular Molecular Medi

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The dressing that promotes scarless healing is essential for both normal function and aesthetics after a wound. With a deeper understanding of the mechanisms involved in scar formation during the wound healing process, the ideal dressing becomes clearer and more promising. For instance, the yes‐associated transcriptional regulator (YAP) has been extensively studied as a key gene involved in regulating scar formation. However, there has been limited attention given to pectolinarin, a natural flavonoid that may exhibit strong binding affinity to YAP, in the context of scarless healing. In this study, we successfully developed a temperature‐sensitive Pluronic@F‐127 hydrogel as a platform for delivering pectolinarin to promote scarless wound healing. The bioactive pectolinarin was released from the hydrogel, effectively enhancing endothelial cell migration, proliferation and the expression of angiogenesis‐related genes. Additionally, a concentration of 20 μg/mL of pectolinarin demonstrated remarkable antioxidant ability, capable of counteracting the detrimental effects of reactive oxygen species (ROS). Our results from rat wound healing models demonstrated that the hydrogel accelerated wound healing, promoting re‐epithelialization and facilitating skin appendage regeneration. Furthermore, we discovered that a concentration of 50 μg/mL of pectolinarin incorporated to the hydrogel exhibited the most favourable outcomes in terms of promoting wound healing and minimizing scar formation. Overall, our study highlights that the significant potential of locally released pectolinarin might substantially inhibit YAP and promoting scarless wound healing.

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Ultrahigh‐performance liquid chromatography coupled with triple quadrupole and time‐of‐flight mass spectrometry for the screening and identification of the main flavonoids and their metabolites in rats after oral administration of Cirsium japonicum DC. extract

Cited by 22 publications

References 23 publications

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Pharmacokinetic and Metabolism Studies of 12-Riboside-Pseudoginsengenin DQ by UPLC-MS/MS and UPLC-QTOF-MSE

Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

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