Ultrahigh performance supercritical fluid chromatography of lipophilic compounds with application to synthetic and commercial biodiesel

Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system

“…In the experiment, nonlinear extraction variation for temperature were concluded when comparing the respective peak area of Schisandrin B, Schisandrin A, Schisandrol B, and Schisandrin in the condition of extraction pressure at 15 MPa, temperature at 40, 50, and 60°C, and time at 3, 4, and 5 min, respectively. Extraction variation tendency of rising first and declining then was shown, which was similar to conventional SFE studies . Therefore, extraction temperature and time were confirmed at 50°C and 4 min.…”

“…Combining SFE with SFC, this type of instrument complements the gap and takes the advantages of both SFE and SFC. SFE presents high ability when extracting low polarity, low boiling point, and lipophilic compounds, which benefited from the extraction fluid of supercritical CO 2 (scCO 2 ) with the characters of similar diffusion coefficient to gas, perfect solubility, and minimal interfacial tension . When different co‐solvents were used, a wide polarity range of extractable analytes could be handled with; meanwhile, samples could be extracted by the scCO 2 in a stable, mild, natural, and non‐chemical polluted condition .…”

Process Development for the Instant Quantification of Lycopene from Agricultural Produces Using Supercritical Fluid Chromatography-Diode Array Detector (SFC-DAD)

“…It is mostly carried out to screen different modifiers on several columns packed with the stationary phase. Optimization of the chromatography parameters for using UPSFC-DAD was performed by the method as designed by Ashraf-Khorassani et al [ 34 ]. Suitability of the available stationary columns named: (1) BEH—3 × 100 mm, 1.7 µm; (2) C18—3 × 100 mm, 1.8 µm; and (3) BEH-2EP—2.1 × 150 mm, 5 µm—with an isocratic elution of a combination of two mobile phases CO 2 /methanol (MeOH) and CO 2 /ethanol (EtOH) at different ratio of 100:0; 90:10; 85:15, and 80:20 ( v / v ) were maintained.…”

Study of UltraHigh Performance Supercritical Fluid Chromatography to measure free fatty acids with out fatty acid ester preparation

“…Following the progress of HPLC as a guide, one can expect lower dispersion, higher pressures and miniaturization in conjunction with polar and nonpolar stationary phases to lead to both reversed phase and normal phase separations with low non-toxic solvent delivery [21][22][23] UHPSFC is capable of analyzing free fatty acids without pre-derivatization as well as the naturally occurring methyl esters. In this regard, the analysis of FFA`s has been recently described by Isaac, et al and the authors of this manuscript for a series of oil samples [24,25]. The separation of FFA species was based on chain length and number of double bonds.…”