Ultrasensitive assay based on a combined cascade amplification by nicking-mediated rolling circle amplification and symmetric strand-displacement amplification

Xu, Huo; Zhang, Yafeng; Zhang, Shuxin; Sun, Mengze; Li, Weihong; Jiang, Yifan; Wu, Zai‐Sheng

doi:10.1016/j.aca.2018.10.004

Cited by 58 publications

(30 citation statements)

References 50 publications

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“…RCA has become increasingly popular in miRNA detection due to its simplicity, specificity, and high sensitivity. In most cases, miRNA works as a ligation template, and the padlock probe will respectively hybridize with the target miRNA which will be ligated by T4 RNA ligase or SplintR enzyme, forming a circular ssDNA, followed by extension around the circle with an external primer or miRNA itself as a primer [[79], [80], [81]], ultimately displacing the conjoined miRNA and continuing to produce long cascaded nucleic acid products. The nucleic acid products can be detected by a variety of signal readout techniques, such as fluorescence, colorimetry, electrochemistry, electrochemiluminescence, SERS, and LAMP.…”

Section: Newly Developed Methodsmentioning

confidence: 99%

Research advances in the detection of miRNA

Tian

et al. 2019

Journal of Pharmaceutical Analysis

291

152

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MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy.

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Section: Newly Developed Methodsmentioning

confidence: 99%

Research advances in the detection of miRNA

Tian

et al. 2019

Journal of Pharmaceutical Analysis

291

152

View full text Add to dashboard Cite

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“…It is also worth noting that miRNA‐21 concentration in these diluted serum samples was 0.5 p m ; that is, 500 f m . This detection limit is comparable to or even better than some detection methods with complex enzymatic recycling amplification (Table S2) . These results indicate that the proposed assay may be useful for analysis of biological fluid samples such as urine and cell culture media to quantify miRNA targets at the f m level.…”

Section: Resultsmentioning

confidence: 53%

A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis

Zhang

Zhao

et al. 2020

Chemistry A European J

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MicroRNAs (miRNAs)m odulate the expression of over 30 %o fm ammalian genes during development and apoptosis, and abnormale xpression of miRNAs may lead to arange of humanp athologies. Therefore, analysis of miRNAs is valuable for disease diagnostics. In this work, an ovel onepot fluorescence derivatization strategy wasd eveloped for miRNA analysis. The mechanism of the derivatization reaction was explored by using instrumental methods, including liquid chromatography,f luorescence spectroscopy,a nd mass spectrometry.H ighly fluorescent N 6 -ethenoadenine (e-adenine) was formed and detached from the miRNA sequence through the reaction of adenine in nucleic acids with 2-chloroacetaldehyde (CAA) at 100 8C. This is the first experimental evidence that the cooperation of formed e-adenine and water-mediatedh ydrogen-bond interaction between the protona tt he 2'-a nd the oxyanion at 3'-positions stabilized the oxocarbenium significantly,w hich makes the depurinationa nd derivatization of miRNA highly effective. Based on this derivatization strategy,afacile and sensitive highperformancel iquid chromatography method was developed for quantitative assay of miRNAs. In combination with magnetic solid-phase extraction( MSPE), the HPLCm ethod was shown to be useful for the determination of microRNAsa t sub-picomolar level in serum samples.[a] Dr.

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“…Then, this DNA complex will be ligated by an enzyme to form a circular single-stranded DNA (ssDNA). An external primer or miRNA itself works as primer to extend on the circular ssDNA, and eventually displace the linked miRNA, then long cascaded products, which are the repeated sequences from circular ssDNA, are produced [14,15,[17][18][19][22][23][24][25][26][27]. However, the detecting of RCA products is exactly laborious and time-consuming, although several kinds of methods based on RCA were designed for simple and specific miRNA expression analysis including MNAzyme-mediated [13],…”

Section: Introductionmentioning

confidence: 99%

“…2 of 11 nicking-mediated [17,27,28] and dumbbell probe-mediated RCA [29]. However, the sensitivity of these RCA-based assays is still generally unsatisfactory, with low levels of miRNA detection, which limits its biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

et al. 2021

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Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection. With the participation of Bst 2.0 DNA Polymerase, the method enabled RCA and LAMP amplification to occur simultaneously without thermal cycling. The limit of detection of RC-LAMP was 500 amol/L, which is comparable to previously reported amplification strategies. Moreover, its upper limit of quantitation was higher and showed a stronger resistance to matrix interference. Therefore, it is possible to detect low concentrations of miRNA in samples by increasing the total RNA added. Owing to its facile detection mode and simple operation, this method has great potential in clinical sample detection.

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Ultrasensitive assay based on a combined cascade amplification by nicking-mediated rolling circle amplification and symmetric strand-displacement amplification

Cited by 58 publications

References 50 publications

Research advances in the detection of miRNA

Research advances in the detection of miRNA

A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

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