Ultrasensitive Colorimetric DNA Detection using a Combination of Rolling Circle Amplification and Nicking Endonuclease‐Assisted Nanoparticle Amplification (NEANA)

Xu, Wei; Xie, Xiaoji; Li, Dawei; Yang, Zhaoqi; Li, Tianhu; Liu, Xiaogang

doi:10.1002/smll.201200263

Cited by 112 publications

(67 citation statements)

References 62 publications

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“…In recent years, enzymes have been widely applied for the development of amplified colorimetric sensors to detect DNA because the mechanisms of various enzymes involve nucleic acids as substrates [194][195][196][197][198][199][200][201]. Liu et al developed an approach to enhance the sensitivity of a DNA sensor by using nicking endonuclease-assisted nanoparticle amplification (NEANA) [194].…”

Section: Increased Aggregate Size: Enzyme-guided Aggregate Formationmentioning

confidence: 99%

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Strategies for enhancing the sensitivity of plasmonic nanosensors

et al. 2015

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Based on the localized surface plasmon resonance (LSPR) of metallic nanoparticles, plasmonic nanosensors have emerged as a powerful tool for biosensing applications. Many detection schemes have been developed and the field is rapidly growing to incorporate new methodologies and applications. Amidst all the ongoing research efforts, one common factor remains a key driving force: continued improvement of high-sensitivity detection. Although there are many excellent reviews available that describe the general progress of LSPR-based plasmonic biosensors, there has been limited attention to strategies for improving the sensitivity of plasmonic nanosensors. Recognizing the importance of this subject, this review highlights recent progress on different strategies used for improving the sensitivity of plasmonic nanosensors. These strategies are classified into the following three categories based on their different sensing mechanisms: (1) sensing based on target-induced local refractive index changes, (2) colorimetric sensing based on LSPR coupling, and (3) amplification of detection sensitivity based on nanoparticle growth. The basic principles and cutting-edge examples are provided for each kind of strategy, collectively forming a unifying framework to view the latest attempts to improve the sensitivity of nanoplasmonic sensors. Future trends for the fabrication of improved plasmonic nanosensors are also discussed.

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Section: Increased Aggregate Size: Enzyme-guided Aggregate Formationmentioning

confidence: 99%

“…Soon after, Liu and his colleagues combined the advantages of the RCA and the NEANA techniques in order to develop a colorimetric sensor for rapid DNA detection (Fig. 12) [196]. In particular, a highly specific padlock oligonucleotide was developed for the RCA reaction.…”

Section: Increased Aggregate Size: Enzyme-guided Aggregate Formationmentioning

confidence: 99%

Strategies for enhancing the sensitivity of plasmonic nanosensors

et al. 2015

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“…[ 1 ] The last decade witnessed major advances in the development of novel DNA biosensors. [ 1c , 1d , 2 ] Different signal transduction techniques including colorimetric, [ 3 ] electrochemical, [ 4 ] fl uorescent, [ 5 ] chemiluminescent, [ 6 ] light scattering, [ 7 ] and mass-based methods [ 8 ] have been utilized to achieve the detection of DNA at trace levels. To realize ultrasensitive detection, several signal amplifi cation strategies have been employed including nanomaterial-based signal amplifi cation, [ 9 ] enzyme catalysis, [ 10 ] and molecular biology-based amplifi cation such as polymerase chain reaction.…”

Section: Doi: 101002/adma201502982mentioning

confidence: 99%

Ultratrace DNA Detection Based on the Condensing‐Enrichment Effect of Superwettable Microchips

et al. 2015

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A sensitive nucleic acid detection platform based on superhydrophilic microwells spotted on a superhydrophobic substrate is fabricated. Due to the wettability differences, ultratrace DNA molecules are enriched and the fluorescent signals are amplified to allow more sensitive detection. The biosensing interface based on superwettable materials provides a simple and cost-effective way for ultratrace DNA sensing.

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“…Consequently targets without the recognition sequence cannot be detected by NEANA. To improve the feasibility of the NEANA for detecting real biological samples with various sequences, RCA was combined with NEANA (Xu et al, 2012). Although this improvement allows NEANA to detect any target sequence, target-specific padlock probes are required; this might result in a high cost when detecting different targets.…”

Section: Introductionmentioning

confidence: 99%