Ultrasensitive electrochemical immunosensor for carbohydrate antigen 72-4 based on dual signal amplification strategy of nanoporous gold and polyaniline–Au asymmetric multicomponent nanoparticles

Fan, Haixia; Guo, Zhaoheng; Gao, Liang; Zhang, Yihe; Fan, Dawei; Ji, Guanglei; Du, Bin; Wei, Qin

doi:10.1016/j.bios.2014.08.043

Cited by 82 publications

(37 citation statements)

References 41 publications

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“…As signal amplification and noise reduction are crucial for achieving low detection limits in clinical immunoassays, researchers have been devoted to proposing new kinds of detection methods or materials to achieve the above goals. Owing to the unique characteristics of nanoparticles, many of them have been used in the signal amplification strategy, such as quantum dots (Zhao et al, 2012;Wen et al, 2013) and metal nanoparticles (Fan et al, 2014;Li et al, 2012). In addition, enzyme labeling (Sheng and Zheng, 2009;Yan et al, 2012) is the most commonly employed labeling method not only for the intrinsic catalytic activity of enzyme but also for its high efficiency and specificity.…”

Section: Introductionmentioning

confidence: 99%

Bifunctional magnetic nanobeads for sensitive detection of avian influenza A (H7N9) virus based on immunomagnetic separation and enzyme-induced metallization

Zhou

Chen

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Bifunctional magnetic nanobeads for sensitive detection of avian influenza A (H7N9) virus based on immunomagnetic separation and enzyme-induced metallization

Zhou

Chen

et al. 2015

Biosensors and Bioelectronics

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“…Calibration curves were obtained in the range 10 3 –5.10 5 cells mL −1 , with a LoD of 400 cells mL −1 . Direct detection of carbohydrate antigens (CA72-4) was also reported by Fan et al [124] using nanoporous gold as substrate and polyaniline-AuNPs (PANi-AuPt) as label. The signaling antibody (Ab 2 ) was adsorbed onto the PANi-AuNPs and served as catalyst for H 2 O 2 oxidation (added in solution).…”

Section: Discussionmentioning

confidence: 63%

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

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Immunosensors have experienced a very significant growth in recent years, driven by the need for fast, sensitive, portable and easy-to-use devices to detect biomarkers for clinical diagnosis or to monitor organic pollutants in natural or industrial environments. Advances in the field of signal amplification using enzymatic reactions, nanomaterials such as carbon nanotubes, graphene and graphene derivatives, metallic nanoparticles (gold, silver, various oxides or metal complexes), or magnetic beads show how it is possible to improve collection, binding or transduction performances and reach the requirements for realistic clinical diagnostic or environmental control. This review presents these most recent advances; it focuses first on classical electrode substrates, then moves to carbon-based nanostructured ones including carbon nanotubes, graphene and other carbon materials, metal or metal-oxide nanoparticles, magnetic nanoparticles, dendrimers and, to finish, explore the use of ionic liquids. Analytical performances are systematically covered and compared, depending on the detection principle, but also from a chronological perspective, from 2012 to 2016 and early 2017.

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“…The drawback here is the possibility of redox species leakage, which would affect the stability of the label-free electrochemical immunosensor. Although previous studies have introduced enzyme catalysis to achieve a higher electrochemical signal, this could increase the complication and expenses of the immunosensor preparation 34–36 . Considering the above situations, it is of great significance to develop a new type of multifunctional substrate that can provide a redox signal, amplify the signal, and immobilise antibodies for use in a label-free electrochemical immunosensor.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Wang

Gao

2017

Sci Rep

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Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays.

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Ultrasensitive electrochemical immunosensor for carbohydrate antigen 72-4 based on dual signal amplification strategy of nanoporous gold and polyaniline–Au asymmetric multicomponent nanoparticles

Cited by 82 publications

References 41 publications

Bifunctional magnetic nanobeads for sensitive detection of avian influenza A (H7N9) virus based on immunomagnetic separation and enzyme-induced metallization

Bifunctional magnetic nanobeads for sensitive detection of avian influenza A (H7N9) virus based on immunomagnetic separation and enzyme-induced metallization

Recent Advances in Electrochemical Immunosensors

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

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