Ultrasound-Augmented Multienzyme-like Nanozyme Hydrogel Spray for Promoting Diabetic Wound Healing

Abstract

Treatment of diabetic foot ulcers (DFU) needs to reduce inflammation, relieve hypoxia, lower blood glucose, promote angiogenesis, and eliminate pathogenic bacteria, but the therapeutic efficacy is greatly limited by the diversity and synergy of drug functions as well as the DFU microenvironment itself. Herein, an ultrasoundaugmented multienzyme-like nanozyme hydrogel spray was developed using hyaluronic acid encapsulated L-arginine and ultrasmall gold nanoparticles and Cu 1.6 O nanoparticles coloaded phosphorus doped graphitic carbon nitride nanosheets (ACPCAH). This nanozyme hydrogel spray possesses five types of enzyme-like activities, including superoxide dismutase (SOD)-, catalase (CAT)-, glucose oxidase (GOx)-, peroxidase (POD)-, and nitric oxide synthase (NOS)-like activities. The kinetics and reaction mechanism of the sonodynamic/sonothermal synergistic enhancement of the SOD-CAT-GOx-POD/NOS cascade reaction of ACPCAH are fully investigated. Both in vitro and in vivo tests demonstrate that this nanozyme hydrogel spray can be activated by the DFU microenvironment to reduce inflammation, relieve hypoxia, lower blood glucose, promote angiogenesis, and eliminate pathogenic bacteria, thus accelerating diabetic wound healing effectively. This study highlights a competitive approach based on multienzyme-like nanozymes for the development of all-in-one DFU therapies.

Functionalized Mesoporous Polydopamine Nanocarrier with Near-Infrared Laser-Trigged NO Release and Photothermal Effects for the Killing of Pathogenic Bacteria

“…Biofilm is not only one of the important causes of bacterial infection, but also one of the major reasons of bacterial resistance. − Biofilm acts as a barrier and many antibiotics cannot penetrate it to kill bacteria inside it, leading to the failure of bacterial infections treatment. , Therefore, the ability to form antibiofilm is of great importance for antibacterial agents. The inhibitory effect of SNP/MPDA on biofilm formation was assessed employing crystalline violet staining . As shown in Figure A, in the control group, a very obvious biofilm formed at the bottom of the hole.…”

Hydrogel-based dressings designed to facilitate wound healing

“…Consequently, these effects significantly expedite the healing process of diabetic wounds. 158 Collectively, these studies underscored the potential of diverse hydrogel-based strategies that incorporate antioxidants and nanoparticles to enhance wound healing, mitigate oxidative stress, and alleviate inflammation.…”

Synthesis of γ-Cyclodextrin-Reduced Fe(III) Nanoparticles with Peroxidase-like Catalytic Activity for Bacteriostasis of Food

“…TMB was utilized to determine the oxidization ability of Fe-γ-CD. The nanozyme exhibited POD-like activity by catalyzing H 2 O 2 and generating toxic • OH, which oxidized colorless TMB to blue ox TMB (Figure a), showing characteristic absorbance at 652 nm . The POD-like activity of Fe-γ-CD was investigated under fixed conditions, including pH (2–8), temperature (20–60 °C), and reaction time (2–150 min).…”