Ultrasound Cell-Labeling Methods

Wu, Yijen; Ye, Qing; Zhang, Haosen; Hitchens, T. Kevin; Ho, Chien

doi:10.1201/b11182-20

Cited by 1 publication

(1 citation statement)

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“…The ability to observe cell migration in living organisms is key to understanding biological processes and developing novel cellular therapies for a large number of diseases that continue to elude traditional therapeutic approaches. There are several imaging modalities that are able to track cells in vivo, such as MRI, singlephoton emission tomography (SPECT), or bioluminescence and fluorescence imaging (1)(2)(3)(4). Of these, MRI has the broadest application because it is useful clinically, is not limited by depth of penetration, has a moderately high resolution, and does not rely on a radioactive tracer.…”

Section: Introductionmentioning

confidence: 99%

Combining perfluorocarbon and superparamagnetic iron‐oxide cell labeling for improved and expanded applications of cellular MRI

Hitchens

Liu

Foley

et al. 2014

Magnetic Resonance in Med

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Purpose The ability to detect the migration of cells in living organisms is fundamental in understanding biological processes and important for the development of novel cell-based therapies to treat disease. MRI can be used to detect the migration of cells labeled with superparamagnetic iron-oxide (SPIO) or perfluorocarbon (PFC) agents. In this study, we explored combining these two cell-labeling approaches to overcome current limitations and enable new applications for cellular MRI. Methods We characterized 19F-NMR relaxation properties of PFC-labeled cells in the presence of SPIO and imaged cells both ex vivo and in vivo in a rodent inflammation model to demonstrate selective visualization of cell populations. Results We show that with UTE3D, RARE and FLASH 19F images one can uniquely identify PFC-labeled cells, co-localized PFC- and SPIO-labeled cells, and PFC/SPIO co-labeled cells. Conclusion This new methodology has the ability to improve and expand applications of MRI cell tracking. Combining PFC and SPIO strategies can potentially provide a method to quench PFC signal transferred from dead cells to macrophages, thereby eliminating false positives. In addition, combining these techniques could also be used to track two cell types simultaneously and probe cell-cell proximity in vivo with MRI.

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Section: Introductionmentioning

confidence: 99%