Ultrastructural and biochemical changes induced by salt stress in Jatropha curcas seeds during germination and seedling development

Alencar, Nara Lídia Mendes; Gadelha, Cibelle Gomes; Gallão, Maria Izabel; Dolder, Mary Anne Heidi; Prisco, José Tarquı́nio; Gomes-Filho, Enéas

doi:10.1071/fp15019

Cited by 23 publications

(21 citation statements)

References 22 publications

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“…Similar results were found by other authors, such as Alencar et al (2015), who observed delay in the reserve mobilization during the germination of Jatropha curcas seeds submitted to salt stress. The metabolic processes of lipid degradation can be highly sensitive to stresses, such as water deficit, salinity, among others (KRASENSKY & JONAK, 2012).…”

Section: Resultssupporting

confidence: 81%

Mobilization of Reserves of Sunflower Seeds Under Saline Stress

Alencar¹,

Otaviano²,

Gomes-Filho³

2017

Anais Do IV Inovagri International Meeting - 2017

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SUMMARY:This study aimed to evaluating salt stress effects on germination of BRS-122 sunflower seeds, through determination of reserve mobilization by cytochemical and biochemical methods. The experiment consisted in the seed germination in control treatment (distilled water) and salt treatment (200 mM NaCl) in germination chamber (BOD) at 25 °C and 12h photoperiod of light. In lipid determination, there was an accumulation of these reserves in cotyledons, in salt conditions, demonstrating a delay in the use of these reserves. Regarding to protein mobilization, a 21.2% reduction was observed for these contents in control treatment at 2DAI, and on other hand, at the final germination period, it was verified an accumulation of proteins. The Na + content was higher in plants exposed to NaCl, whereas K + content was reduced over time in control treatment, however, in salt conditions, there was an accumulation of this ion. We assumed that the salinity exposure in sunflower seeds led to Na + ion accumulation, which negatively affected the reserve mobilization of these seeds. KEYWORDS: Germination, Helianthus annus L., salinity MOBILIZAÇÃO DE RESERVAS DE SEMENTES DE GIRASSOL SUBMETIDAS AO ESTRESSE SALINORESUMO: Nesse trabalho, objetivou-se estudar os efeitos do estresse salino sobre a germinação de sementes de girassol BRS-122, avaliando a mobilização de reservas ao longo da germinação por meio da determinação de compostos de reserva por métodos citoquímicos e bioquímicos. O experimento consistiu na germinação das sementes em tratamento controle (água destilada) e tratamento salino (200 mM de NaCl) em câmara de germinação (BOD) com temperatura de 25°C e fotoperíodo de 12h. Na determinação de lipídios, observou-se que no tratamento salino houve um acúmulo de lipídios nos cotilédones, demonstrando retardo na utilização dessas reservas. Em relação a mobilização proteica dos cotilédones para o eixo

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Section: Resultssupporting

confidence: 81%

Mobilization of Reserves of Sunflower Seeds Under Saline Stress

Alencar¹,

Otaviano²,

Gomes-Filho³

2017

Anais Do IV Inovagri International Meeting - 2017

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“…Some authors have reported the negative effect on germination of seeds subjected to saline stress, such as for pinhão-manso (Alencar et al, 2015) and cajueiro anão precoce (Voigt et al, 2009;Marques et al, 2013). In addition to the negative effect on the germination parameters, these authors also mentioned that the exposure to saline stress contributes to delay of the mobilization of reserves, which compromises the development of the seedlings, affecting their development.…”

Section: Methodsmentioning

confidence: 99%

“…Physiological responses of plant species to abiotic stresses, such as water and saline stress, have been widely repported by several authors (Munns;Tester, 2008;Voigt et al, 2009;Marques et al, 2013;Alencar et al, 2015). The physiological mechanisms of tolerance to these stresses have been studied in plant species during the germinative phase (Voigt et al, 2009, Marques et al, 2013, Alencar et al, 2015.…”

Section: Piptadenia Stipulacea Mimosa Caesalpiniifolia Benth Caesalmentioning

confidence: 99%

“…The physiological mechanisms of tolerance to these stresses have been studied in plant species during the germinative phase (Voigt et al, 2009, Marques et al, 2013, Alencar et al, 2015. However, little is known about mechanisms of seed adaptation of native species, especially of the northeastern semiarid region, with conditions of water restriction and salinity in the soil, and how these seeds can initiate their vegetative development in this natural environment.…”

Section: Piptadenia Stipulacea Mimosa Caesalpiniifolia Benth Caesalmentioning

confidence: 99%

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Germination of Caatinga Native Species Under Salt Stress Conditions

Alencar¹,

Oliveira²,

Mota³

et al. 2017

Anais Do IV Inovagri International Meeting - 2017

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ABSTRACT:The physiological tolerance mechanisms of some plants to salt and water stress during germination are already well known. However, little is known about these mechanisms in native Caatinga species, based on this, this study aimed to evaluate the influence of stress on seed germination of the Caatinga native species, Piptadenia stipulacea (jurema blanca), Mimosa caesalpiniaefolia (sabiá), Caesalpinea ferreae (jucá) and Pityrocarpa moniliformis (catanduva). Salt treatments corresponded to 0; 40.36; 80.72; 121.09 and 165.41 mM NaCl.The following variables were evaluated: percentage of germination (% G), germination speed index (GSI) and mean germination time (MGT). No significant differences were found for %G of P. stipulaceae; C. ferreae and P. moniliformis for salt treatments. Regarding GSI, the analyzed species presented differences between treatments, except for. P. stipulaceae and P. moniliformis showed to be the most tolerant to salt stress during the germination phase, because they presented high levels of germination, even in the higher doses of salt.KEYWORDS: seed development, salinity, tolerance. GERMINAÇÃO DE ESPÉCIES NATIVAS

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“…In Cereus jamacaru, Alencar et al (2012) discovered that lipids were the main reserve in seeds that were stored in cotyledons while soluble sugar and starch were the minor reserves. In Jatropha curcas seeds, it was determined that the endosperm lipids represent the most abundant reserve and proteins were the secondmost important reserve (Alencar et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Histochemical changes during the ontogeny of malagueta and biquinho pepper seeds

Abud

Araújo

et al. 2017

Acta Sci. Agron.

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ABSTRACT. This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol's iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat cells was observed. Proteins and lipids were the main reserve compounds of malagueta and biquinho pepper seeds stored in the embryo and endosperm. Starch served as a transient reserve during embryo development. The lipid reserves were observed in embryo and endosperm cells from 25 until 100 DAA. At 100 DAA, the protein contents of the malagueta and biquinho pepper seeds were 0.33 and 0.62 g g Alterações histoquímicas durante a ontogenia de sementes de pimenta malagueta e biquinho RESUMO. Objetivou-se acompanhar as alterações estruturais, a deposição de reservas e determinar a composição química de sementes de pimenta malagueta e biquinho durante a sua ontogênese. Frutos foram coletados aos 25, 40, 55, 70, 85 e 100 dias após a antese (DAA). Após a coleta, as sementes foram extraídas e fixadas em FAA 50 e, armazenadas em álcool 70%. Posteriormente, foram incluídas em Historesina Metacrilato e seccionadas em micrótomo. Os cortes foram submetidos à coloração com Azul de Toluidina, para análise anatômica e a análise histoquímica realizada com XylidinePonceau, Periodic acid Schiff (PAS) e Lugol. Para reação com Floroglucina Ácida e Sudam III, foram realizados cortes em criomicrótomo. Verificou-se que houve lignificação progressiva das células do tegumento. As proteínas e os lipídeos constituem os principais compostos de reserva das sementes de pimenta malagueta e biquinho, estocados no embrião e endosperma. O amido constitui uma fonte transitória de reserva para o desenvolvimento do embrião. As reservas lipídicas são observadas nas células do embrião e do endosperma dos 25 aos 100 DAA. Aos 100 DAA, as sementes de pimenta malagueta e biquinho apresentam concentração de proteínas de 0,33 e 0,62 g g -1 M.S. e lipídeos de 0,30 e 0,25 g g -1 M.S. respectivamente.

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Ultrastructural and biochemical changes induced by salt stress in Jatropha curcas seeds during germination and seedling development

Cited by 23 publications

References 22 publications

Mobilization of Reserves of Sunflower Seeds Under Saline Stress

Mobilization of Reserves of Sunflower Seeds Under Saline Stress

Germination of Caatinga Native Species Under Salt Stress Conditions

<b>Histochemical changes during the ontogeny of <i>malagueta</i> and <i>biquinho</i> pepper seeds

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