Ultrastructural changes and nestin expression accompanying compensatory renal growth after unilateral nephrectomy in adult rats

Eladl, Mohamed Ahmed; Elsaed, Wael M.; Atef, Hoda; El-Sherbiny, Mohamed

doi:10.2147/ijnrd.s121473

Cited by 9 publications

(8 citation statements)

References 76 publications

(83 reference statements)

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“…Compensatory and adaptive reactions of a single kidney after removal from the body of the contralateral form part of the general system of adaptation of the body in cases of its damage. We have established an increase in the mass of the right kidney of rats after the removal of the left kidney, which reached its peak after 30 days, consistent with the data of other authors [7]. By comparing the mass of a single kidney remaining after contralateral nephrectomy, with the weight of two kidneys of the control group in a given experiment period, we found that the index of hypertrophy of the kidney during the postoperative period ranged from 52.35% to 65.22The maximum index of kidney hypertrophy was observed in the period of 30 days.…”

Section: Discussionsupporting

confidence: 92%

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Changes in the sizes of the kidney after contralateral nephrectomy in the experiment

Моnastyrskyi

2018

Rep. of Morph.

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The evaluation of renal measurements such as length, width and thickness, volume is important in the diagnosis and treatment of many renal disorders, since there is a close relationship between the sizes of the kidneys and its function. The purpose of the study was to establish and compare during the postoperative period changes in the mass and sizes of the kidney left after nephrectomy in the sexually mature male rats. An experimental study was carried out on 84 sexually mature white male rats weighing 178-194 grams. Animals were divided into two groups: control (42 rats) and experimental (42 rats). In the control group, the animals under ketamine anesthesia were followed by an abdominal cavity dilution, after which the abdominal wall was applied in a row. All animals of the experimental group performed surgical intervention – nephrectomy of the left kidney. The animals were withdrawn from the trial by intra-pleural administration of thiopental-sodium 50 mg/kg after 7, 14, 21, 30, 90 days after nephrectomy. Macroscopic evaluation and description of the kidneys of animals was performed after their removal. Their weight was determined on the laboratory scale of HLR-200 up to 0.1 mg, and the length, width and thickness of the organ were measured with the help of a caliper to an accuracy of 0.05 mm. Calculated the volume of the kidney. The statistical analysis of the obtained results was carried out using the program STATISTICA 5.5 using parametric methods for evaluating the results. It was established that the mass, length, width, thickness and volume of the kidney of animals in the experimental group, as compared with the control group, were statistically significantly higher in all terms of observation. The fraction of the growth of the width and thickness of the single kidney of animals in the experimental group compared with the control animals in the animals was statistically significantly greater than the proportion of kidney growth during the postoperative period. It was found that the largest increase in the mass and thickness of the kidney, as compared with the control group, was observed after 30 days of the postoperative period, and the greatest increase in the length and width of the kidney, as compared with the control group, was observed after 14 days of the postoperative period.

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Section: Discussionsupporting

confidence: 92%

“…In the future, it is planned to compare changes in organometric parameters of the kidney, remaining after nephrectomy, in non-sexually mature and mature animals. внутрішньоплеврального введення тіопенталу-натрію 50 мг/кг через 7,14,21,30,90…”

Section: Discussionunclassified

Changes in the sizes of the kidney after contralateral nephrectomy in the experiment

Моnastyrskyi

2018

Rep. of Morph.

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“…The extensive changes either in light microscopy or in the ultrastructural examination in groups IV and VI were attributed to vasoactive mediators induced by oxidative stress [29,49]. The protective effect of E2 was approved histopathologically by minimal glomerular, PT and DT changes when compared to the OVX rats.…”

Section: Discussionmentioning

confidence: 99%

“…The slides were digitized and saved as TIFF files. The results obtained from the images were analyzed on IntelR Core I3R built computer by using Video Test Morphology R software (Saint-Petersburg, Russia) with a particular built-in routine for calibrated immune staining quantification in relation to the total sectional area [49].…”

Section: Methodsmentioning

confidence: 99%

Potential role of estradiol in ovariectomy-induced derangement of renal endocrine functions

2019

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Menopause is an important physiological event associated with structural and functional changes in the kidneys. An animal model of bilateral ovariectomy was used to study the effects of estrogen depletion, replacement and antiestrogen on renal structure and endocrine function. Sixty female rats were divided into six groups; group I was the control group, the remaining five groups underwent ovariectomy: group II received no treatment. The other groups received estradiol in group III, tamoxifen in group IV, estradiol followed by tamoxifen in group V and tamoxifen followed by estradiol in group VI. Serum creatinine, blood urea nitrogen, and endocrine functions of kidney were measured. Tissue samples were examined both microscopically for beta estrogen receptors and ultrastructurally for cell changes. Groups II, IV & VI showed a significant increase in creatinine, blood urea nitrogen, renal malondialdehyde, renal erythropoietin, plasma renin and plasma prostaglandin E2 and a significant decrease in renal antioxidants and serum vitamin D3. Groups III &V had a significant decrease in creatinine, blood urea nitrogen, renal malondialdehyde and renal erythropoietin with an increase in renal antioxidants, plasma prostaglandin E2 and serum vitamin D3. Histopathological and ultrastructural examinations revealed atrophic tubular changes in group II. The changes were less marked in groups III &V and more extensive in groups IV & VI. Estrogen receptor beta staining showed progressively increased expression in the absence of estrogen. Structural and most endocrine functions of the kidney were significantly affected by estradiol deficiency. Estradiol replacement exhibited a protective effect on renal tissue and endocrine functions.

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“…According to the literature, experiments conducted on rats have shown that these animals can be used as a model for studying the course of recovery of the body after nephrectomy and resection of the kidney and the selection of corrective therapy, aimed at the earliest adaptation of the organism to existence in the new conditions [10,11]. Male rats were more sensitive and less adapted to renal excretion [24].…”

Section: Methodsmentioning

confidence: 99%

Organometric parameters of the remaining kidney after removal of the contralateral in immature rats

Моnastyrskyi

2018

Rep. of Morph.

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The processes of compensatory hypertrophy of the kidney to the period of puberty are insufficiently studied. Changes in renal parameters after contralateral removal are of scientific interest. The purpose of the study was to establish and compare changes in organometric parameters of the kidney that remained after nephrectomy in non-sexually adult male rats and in sexually mature male rats. Experimental study was performed on 84 non-adult white male rats weighing 53-71 grams, which were kept on a standard vivarium diet. All animals were divided into two groups (42 in each): the first - control, the second - the experimental group. In the control group, the abdominal cavity was opened under ketamine anesthesia, after wich the abdominal wall was sutured in layers. All animals of the experimental group performed surgical intervention - nephrectomy of the left kidney. Animals were withdrawn from the trial by intra-pleural administration of thiopental-sodium 50 mg/kg after 7, 14, 21, 30, 90 days after nephrectomy. Macroscopic evaluation and description of the kidneys of animals was performed after their removal. The statistical analysis of the obtained results was carried out using the “STATISTICA 5.5” program, using parametric and non-parametric methods for evaluating the obtained results. It was found that in the immature rats, the weight of animals in the experimental group (after nephrectomy) was statistically significantly lower in all terms of the observation compared to the control group, and the weight of the kidney of animals in the experimental group, on the contrary, was statistically significantly higher in all terms of observation. The length, width, thickness, volume of kidneys of animals of the experimental group, as well as the magnitude of increment of these parameters with each term of observation are determined. The greatest increase in organometric parameters of the kidney was observed after 30 days of the postoperative period (p<0.05). Subsequently, on the 60th and 90th day, the magnitude of the increase in organometric parameters of the kidney significantly decreased. The index of hypertrophy of the kidney remained unpaired by nephrectomy, which varied from 52.24% to 63.21%. The highest rates of hypertrophy were observed between the 21st and 30th days.

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Ultrastructural changes and nestin expression accompanying compensatory renal growth after unilateral nephrectomy in adult rats

Cited by 9 publications

References 76 publications

Changes in the sizes of the kidney after contralateral nephrectomy in the experiment

Changes in the sizes of the kidney after contralateral nephrectomy in the experiment

Potential role of estradiol in ovariectomy-induced derangement of renal endocrine functions

Organometric parameters of the remaining kidney after removal of the contralateral in immature rats

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