Ultrastructural Changes in Cotyledons of Pineapple Guava (Myrtaceae) During Somatic Embryogenesis

Canhoto, Jorge; Mesquita, J. F.; Cruz, Gil S.

doi:10.1006/anbo.1996.0149

Cited by 55 publications

(49 citation statements)

References 28 publications

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“…The usual RER concentric profile, seen during the initial stages of G. max and B. forficata meristemoids, may be involved in protein synthesis in response to rapid cellular growth, and not associated with the potential of a morphogenetic system, as suggested for embryogenic processes by Radojevic et al (1975), Vujicic et al (1979), and Canhoto et al (1996). As can be seen in this study, reporting the occurrence of RER concentric profiles in organogenetic systems should be more common.…”

Section: Discussionsupporting

confidence: 58%

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Ultrastructural analysis of in vitro direct and indirect organogenesis

Appezzato‐da‐Glória¹,

Machado²

2004

Rev. bras. Bot.

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-(Ultrastructural analysis of in vitro direct and indirect organogenesis). This is a comparative study of the ultrastructural characteristics of the cells involved in the organogenesis in vitro of Bauhinia forficata Link (indirect system) and Glycine max (L.) Merrill (direct system). B. forficata calli after 30 days culture and G. max meristemoids after 10 days culture were prepared for ultrastructural analysis using conventional methods. Concentrically arranged rough endoplasmic reticulum (RER) and plastids containing starch grains were seen during G. max and B. forficata organogenesis. The amitotic process, the presence of plastids around the nucleus and nuclear envelope with conspicuous pores were found in B. forficata.Key words -amitosis, leguminous, meristemoids, morphogenesis RESUMO -(Análise ultra-estrutural da organogênese in vitro direta e indireta). Este é um estudo comparativo das características ultra-estruturais das células envolvidas na organogênese in vitro de Bauhinia forficata Link (sistema indireto) e Glycine max (L.) Merrill (sistema direto). Calos de B. forficata, após 30 dias de cultura, e meristemóides de G. max, após 10 dias de cultura, foram preparados para as análises ultra-estruturais usando métodos convencionais. Retículo endoplasmático rugoso (RER) arranjado concentricamente e plastídeos contendo grãos de amido foram observados durante a organogênese de G. max e B. forficata. O processo amitótico, a presença de plastídeos ao redor do núcleo e de envelope nuclear com poros conspícuos foram observados apenas em B. forficata.

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Section: Discussionsupporting

confidence: 58%

“…In G. max, the mitochondria are abundant and contain an electron dense matrix and well-developed cristae. These features have been described in embryogenic systems as being associated with higher metabolic activity, usually related to higher respiratory rate (Profumo et al 1987, Fransz & Schel 1991, Jasik et al 1995, Canhoto et al 1996.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural analysis of in vitro direct and indirect organogenesis

Appezzato‐da‐Glória¹,

Machado²

2004

Rev. bras. Bot.

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“…As observed by Canhoto et al (1996), the cells of the Revista Árvore, Viçosa-MG, v.34, n.5, p.789-796, 2010 pro-embryos usually have some characteristics of the meristematic cells, however they can be so covered by vacuoles that there is only a fine cytoplasm between the tonoplast and the plasmatic membrane. The abundant presence of Mitochondria with electrodense matrix and well-developed crests and with round shape was observed in these cells and has also been associated to the embryogenic systems, related to the high metabolic activity due to high respiratory rates (Canhoto et al 1996).…”

Section: Discussionmentioning

confidence: 94%

“…The large quantity of mitochondria, mostly with an elongated shape, also indicated the non-embryogenic caracteristics. According to Canhoto (1996), non-embryogenic cells have longish mitochondria, while the mitochondria in embryogenic cells are rounded. However, Skoog and Miller (1957) affirmed that the non-embryogenic cells are able to develop meristematic cells and can, under satisfactory conditions, bring forth a new plant.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Stein

Paiva

Vargas³

et al. 2010

Rev. Árvore

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-Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis. Keywords: Anthers, Ovaries e Nodal segments. ANÁLISE ULTRAESTRUTURAL DE CALOS DE INGAZEIRO (

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“…Although their fruits are edible, these trees are mainly used for ornamental purposes in Japan. In contrast to other plant structures (Canhoto et al, 1996;Kitayama et al, 1997;O'Brien, 1994;O'Brien et al, 1996), there are few if any reports on the root structure of members of the Myrtaceae. The development of suberin lamellae and Casparian strips in the endodermis, root lignification, and thickening of the cortical cell wall associated with root maturation are particularly important in perennial tree roots.…”

Section: Introductionmentioning

confidence: 99%

Formation of Endodermis-like Cells with Casparian Strip and Thick Wall Cells Derived from Pericycle in the Roots of Feijoa sellowiana (Myrtaceae)

Nii

Ohtsuka

et al. 2012

J. Japan. Soc. Hort. Sci.

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Two anatomical features of cells derived from the pericycle of the roots of Feijoa sellowiana are reported: (1) appearance of endodermis-like cell layer with a Casparian strip, which was confirmed by histological examination; (2) occurrence of a thick wall cell between endodermis-like cells. These features were considered to develop as follows: two tangential cell walls appeared successively in the pericycle cells, resulting in the formation of three cell layers. The walls of the cells of the outer layer, adjacent to the endodermis, became thicker on the interior side of the cell and heavily encrusted with lignin. The walls of the cells in the middle layer derived from the pericycle became suberized and developed into Casparian strips. These cells subsequently developed into endodermis-like cells. The innermost cell layer derived from the pericycle retained their original morphology for a while, but again divided tangentially and developed as mentioned above. Consequently, in aged roots, there were several layers, in which endodermis-like cells with Casparian strips alternated with cells with thick inner walls. The tangential width of endodermis-like cells reduced in sequence from the outer to inner layer. It is quite important to know how the anatomical features of feijoa trees appear under different soil conditions.

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Ultrastructural Changes in Cotyledons of Pineapple Guava (Myrtaceae) During Somatic Embryogenesis

Cited by 55 publications

References 28 publications

Ultrastructural analysis of in vitro direct and indirect organogenesis

Ultrastructural analysis of in vitro direct and indirect organogenesis

Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Formation of Endodermis-like Cells with Casparian Strip and Thick Wall Cells Derived from Pericycle in the Roots of Feijoa sellowiana (Myrtaceae)

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