Ultrastructural Changes in Granulosa Cells in Porcine Antral Follicles Undergoing Atresia Indicate Apoptotic Cell Death.

Sugimoto, Miki; Manabe, Noboru; Kimura, Yuichi; Myoumoto, Akira; Imai, Yuzuru; Ohno, Hirohisa; Miyamoto, Hajime

doi:10.1262/jrd.44.7

Cited by 40 publications

(36 citation statements)

References 27 publications

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“…For example, follicular atresia in mice (Byskov, 1974), rats (Osman, 1985;Hirshfield, 1988), pigs (Sugimoto et al, 1998), goats (Garcia et al, 1997), and yaks (Cui and Yu, 1999) was always divided into two or three stages. For guinea pigs, however, it has been difficult to provide uniform classification criteria because there are unique morphologic changes in the atretic process.…”

Section: Introductionmentioning

confidence: 99%

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:There is a lack of appropriate classification criteria for the determination of atretic follicles in guinea pigs. In the present study, new criteria were established based on the latest morphologic criteria for cell death proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009. Ovaries of guinea pigs were sampled on different stages of estrous cycle, and the morphologic observations of atretic follicles were investigated in serial sections. The results showed that the process of follicular atresia could be classified into four continuous stages: (1) the granulosa layer became loose, and some apoptotic bodies began to appear; (2) the granulosa cells were massively eliminated; (3) the theca interna cells differentiated; and (4) the residual follicular cells degenerated. In addition, the examination revealed that these morphologic criteria were accurate and feasible. In conclusion, this study provides new criteria for the classification of atretic follicles in guinea pigs, and this knowledge can inform future research in the area.

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Section: Introductionmentioning

confidence: 99%

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Wang

Liu

Tian

et al. 2010

J. Zhejiang Univ. Sci. B

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“…Manabe et al (1996aManabe et al ( , 1997Manabe et al ( , 1998 indicate that the degeneration of atretic follicles in mammalian ovaries can be explained, at least in part, by apoptotic cell death of granulosa cells. In the early stage of atresia in pig ovaries, apoptosis demonstrated histochemically and ultrastructually was seen in scattered granulosa cells located on the inner surface of the follicular wall, but not in cumulus cells, oocytes or the cells of internal or external theca layers (Manabe et al, 1996a;Sugimoto et al, 1998). However, the apoptotic signal transducing pathway system that mediates apoptosis in granulosa cells of pig ovaries is not known.…”

Section: Introductionmentioning

confidence: 99%

Expression and activity of Apaf1 and caspase-9 in granulosa cells during follicular atresia in pig ovaries

Matsui¹

2003

Reproduction

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Apoptosis in granulosa cells plays a crucial role in ovarian follicular atresia, but the intracellular regulating mechanism, especially the mitochondrion-dependent apoptosis signalling pathway, is still largely unknown. This study examined whether the mitochondrial pathway is associated with granulosa cell apoptosis during atresia in pig ovaries. Both mRNAs of caspase-9 and apoptotic protease-activating factor 1 (Apaf1), which are major signal transducing components in the mitochondrial pathway, were detected in granulosa cells in healthy, early atretic and progressed atretic follicles by RT-PCR. No changes in the expression of Apaf1 mRNA were seen during follicular atresia, but the expression of caspase-9 mRNA increased during atresia. Apaf1 protein was steadily detected in granulosa cells prepared from healthy, early atretic and progressed atretic follicles by western blot analysis, but high expression of the precursor of caspase-9 (procaspase-9) was detected only in granulosa cells of healthy follicles. Decreased procaspase-9 protein was demonstrated during follicular atresia. Proteolytic activity of caspase-9 increased during atresia, in agreement with the diminution of procaspase-9 protein. Intensive expression of caspase-9 mRNA was demonstrated in the granulosa cells of early atretic and progressed atretic follicles but not in those of healthy follicles. These results indicate that the mitochondrial signalling pathway, which is mediated by Apaf1 and caspase-9, plays a crucial role in determining the fate of granulosa cells during atresia in pig ovaries.

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“…Recent findings have suggested that apoptosis, originally described by Kerr et al [4], is the key mechanism underlying ovarian follicular atresia. Apoptotic cell death of granulosa cells of rabbit Graafian follicles with atresia was first observed deoxynucleotidyl transferase (TdT)-mediated biotinylated deoxyuridine triphosphate nick endlabeling (TUNEL) method, and conventional electron microscopic analysis was also performed [9]. In healthy follicles, no apoptotic cells were observed among granulosa or cumulus cells, interna or externa theca cells, or oocytes.…”

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confidence: 99%

Species-specific Differences in Apoptotic Cell Localization in Granulosa and Theca Interna Cells during Follicular Atresia in Porcine and Bovine Ovaries.

Nakayama¹,

Manabe²,

Nishihara³

et al. 2000

J. Reprod. Dev.

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Abstract. Species-specific differences in the process of apoptosis in granulosa and theca layers during follicular atresia in porcine and bovine ovaries were investigated by in situ DNA 3'endlabeling (TUNEL) at the level of individual cells. In porcine ovaries, granulosa cells located on the inner surface of the granulosa layer appeared to undergo apoptosis at the first stage, followed by neighboring granulosa cells. In the contrast, granulosa cells located on the middle region appeared to undergo apoptosis at the first stage in bovine ovaries. In both porcine and bovine ovaries, detachment and degeneration of the granulosa cell layer and fragmentation of basement membrane occurred in the follicles at advanced stage of atresia. In the ovaries of both species, theca interna cells were still intact at the stage of early atresia and then apoptotic cells appeared in the late to final stages of atresia, but no apoptotic cells were observed in theca externa layers during follicular atresia. The present findings indicated that apoptosis occurring in granulosa cells is an initial symptom of follicular atresia in the ovaries of both species, and that the apoptosis inducing factor(s) and/or survival factor(s) for the granulosa cells may be different between porcine and bovine ovaries.

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Ultrastructural Changes in Granulosa Cells in Porcine Antral Follicles Undergoing Atresia Indicate Apoptotic Cell Death.

Cited by 40 publications

References 27 publications

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Morphologic observation and classification criteria of atretic follicles in guinea pigs

Expression and activity of Apaf1 and caspase-9 in granulosa cells during follicular atresia in pig ovaries

Species-specific Differences in Apoptotic Cell Localization in Granulosa and Theca Interna Cells during Follicular Atresia in Porcine and Bovine Ovaries.

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