Ultrastructural changes in micropylar and granulosa cells during in vitro oocyte maturation in the medaka, <i>Oryzias latipes</i>

Nakashima, Seiko; Isobe, Takashi

doi:10.1002/jez.1402700608

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…The zona pellucida of teleosts oocytes is complex, usually consisting of layers crossed by pores or channels (Selman and Wallace, ). It is interrupted at the animal pole region by a specialized opening, the micropyle, which allows the passage of sperm in fertilization (Nakashima and Iwamatsu, ) and its morphology varies between species (Rizzo et al., ). Follicular cells surround the oocyte and its external morphology may vary depending on the oocyte's development (Guraya, ).…”

Section: Introductionmentioning

confidence: 99%

Ovarian Structure and Oogenesis of Catfish Pimelodella vittata (Lütken, 1874) (Siluriformes, Heptapteridae)

Sales

Santos

Arantes

et al. 2012

Anat Histol Embryol

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The morphology of the ovaries and oogenesis of Pimelodella vittata were studied using anatomical and histological techniques to provide information of its reproductive biology. Eighty adult females were captured trimonthly during the period November 2005 to October 2006. The ovaries are paired, saculiform organs, which are coated with tunica albuginea and contain ovigerous lamellae, where the oocytes develop before being released into the ovarian lumen and following the ovarian duct until reaching the genital papilla. Oogenesis was divided into stages based on the alterations to the nucleus, ooplasm and surrounding follicular layers. Oogonia form groups from the germinal epithelium have asynchronous development and differentiate into initial perinucleolar oocytes. The formation of the zona pellucida is initiated in the advanced perinucleolar oocytes reaching a thickness of 1.46±0.58 μm in the vitellogenic oocytes. The follicular cells are squamous in perinucleolar oocytes, become cubical in the pre-vitellogenic oocytes and prismatic in the vitellogenic oocytes with a height of 11.20±4.74 μm. The histochemical reactions indicate that zona pellucida, cortical alveoli and yolk globules contain neutral glycoproteins and the follicular cells contain neutral glycoproteins in association with carboxylated and sulphated glycoconjugates. Statistical analyses showed significant differences in the diameter of the oocytes and follicular cells height as oocytes matured. This study represents the first data about the ovarian structure and oogenesis of this species.

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Section: Introductionmentioning

confidence: 99%

Ovarian Structure and Oogenesis of Catfish Pimelodella vittata (Lütken, 1874) (Siluriformes, Heptapteridae)

Sales

Santos

Arantes

et al. 2012

Anat Histol Embryol

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“…3d). Several authors have reported that, until the initiation of oocyte maturation, the micropylar canal is plugged with cytoplasmic protrusion of a large, mushroomshaped micropylar cell (Takano and Ohta 1982;Kobayashi and Yamamoto 1985;Iwamatsu et al 1993b;Nakashima and Iwamatsu 1994). Bodies of the micropylar cell and nearby granulosa cells exert mechanical pressure on the external surface of the growing oocyte and thus participate in the formation of the micropylar vestibule.…”

Section: Discussionmentioning

confidence: 98%

Morphology of unfertilized mature and fertilized developing marine pelagic eggs in four types of multiple spawning flounders

et al. 2010

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Starry flounder Platichthys stellatus, spotted halibut Verasper variegates, turbot Scophthalmus maximus, and Japanese flounder Paralichthys olivaceus are four commercially cultivated multiple spawning flounders that spawn pelagic eggs. Through appropriate light and scanning electron microscope processing, the shape and surface structures (such as micropyle, pores, pore density, and paten) of unfertilized mature and fertilized developing eggs of the four species were observed and measured. First, individual or intraspecific comparisons of the surface structures of eggs at different developmental stages were made. Second, interspecific differences among the four species at the same developmental stage of unfertilized mature eggs were statistically computed and analyzed through one-way analysis of variance and hierarchical cluster analysis. Eggs of the same species collected at different stages of development tend to be different in morphology. Smoothing of the convoluted egg envelope surface and closure of the micropyle to serve as a final step of the polyspermy-preventing reaction are common after fertilization. Based on detailed morphology of micropyle of just-mature fertilizable eggs, turbot, starry flounder, and Japanese flounder each have a micropyle with a long canal but no distinct micropylar vestibule, type III of Riehl and Götting (Arch Hydrobiol 74:393-402, 1974). In contrast, spotted halibut has a micropyle with a distinct flat micropylar vestibule and a long canal, type II. Envelope surface microstructures, especially those in the micropyle region, are useful characters for egg identification among the four species. Cluster analysis using selected egg characters indicated the highest similarity between turbot and Japanese flounder and that starry flounder is obviously more similar to turbot and Japanese flounder than to spotted halibut.

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“…As a general rule, viviparous fishes are characterized by a very thin zona pellucida, as recorded in viviparous scorpaenoids, such as Sebastes paucispinis, in which the zona pellucida was 1 µm thick [ 67 , 68 ]. If, in H. dactylopterus , the thickness of zona pellucida can be justified with its reproductive mode (e.g., nourishment of embryos), in a species of the genus Scorpaena , the reduction of thickness could imply loss of mechanical protection (essential when spawning is pelagic), which should be alternatively provided by the gelatinous matrix that encloses the eggs, as also reported by [ 11 ] in S. notata .…”

Section: Discussionmentioning

confidence: 99%

Investigating the Ovarian Microstructure in the Genera Helicolenus and Scorpaena (Teleostei, Sub-Order Scorpaenoidei) with Implications for Ovarian Dynamics and Spawning

Porcu

Lai

Bellodi

et al. 2022

Animals

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The sub-order Scorpenoidei appears to be particularly interesting due to the presence of intermediate stages between oviparity and viviparity in several species. The present study aims to describe the ovarian morphology, using a histological and histochemical approach, in four ovuliparous species belonging to Scorpaena genus compared with a zygoparous species, H. dactylopterus, focusing also on the assessment of the ovarian dynamics in the populations of such species in Sardinia waters (central–western Mediterranean). Ovarian sections of all species were examined using light microscopy. All species showed a specialized ovary, cystovarian type II-3, strictly related to the production of gelatinous matrices surrounding the eggs. Some microscopic peculiarities in the oogenesis process were found: thin zona pellucida, small and low cortical alveoli, and a specialized ovarian wall during the spawning period. All species analyzed were batch-spawners with an asynchronous ovarian organization. A continuous recruitment of oocytes and the occurrence of de novo vitellogenesis was also observed. During the spawning period, low atresia intensity was detected, while a marked increase in this intensity found in the ovaries at the end of spawning season. Our observations may support an indeterminate fecundity type for these species.

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Ultrastructural changes in micropylar and granulosa cells during in vitro oocyte maturation in the medaka, Oryzias latipes

Cited by 7 publications

References 12 publications

Ovarian Structure and Oogenesis of Catfish Pimelodella vittata (Lütken, 1874) (Siluriformes, Heptapteridae)

Ovarian Structure and Oogenesis of Catfish Pimelodella vittata (Lütken, 1874) (Siluriformes, Heptapteridae)

Morphology of unfertilized mature and fertilized developing marine pelagic eggs in four types of multiple spawning flounders

Investigating the Ovarian Microstructure in the Genera Helicolenus and Scorpaena (Teleostei, Sub-Order Scorpaenoidei) with Implications for Ovarian Dynamics and Spawning

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