Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract

Yoshihara, Eidi; Minho, Alessandro Pelegrine; Tabacow, Victor Bittencourt Dutra; Cardim, Sérgio Tosi; Yamamura, Milton Hissashi

doi:10.5433/1679-0359.2015v36n6p3763

Cited by 16 publications

(18 citation statements)

References 15 publications

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“…Yoshihara et al (2014) required 100 mg mL -1 of a commercial extract of Acacia mearnsii for 97.1% inhibition of larval migration. In addition to the anthelmintic action, the extract caused ultrastructural changes in adult parasites H. contortus after contact in vitro and in vivo (Yoshihara et al, 2015). For flavonoids and tannins, the highest levels were found in the concentration of 3 mg mL -1 .…”

Section: Resultsmentioning

confidence: 97%

Anthelmintic and antioxidant potential of Fagopyrum esculentum Moench in vitro

Gonçalves¹,

Debiage²,

Yoshihara³

et al. 2016

Afr. J. Agric. Res.

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One of the limiting factors of sheep breeding is helminth infection, mainly resulting in poor weight gain. The combination of parasite control strategies, including the use of medicinal herbs can reduce the use of chemical anthelmintics. Tanniferous plants, by having phenolic compounds, mainly condensed tannins were associated with anthelmintic action. Fagopyrum esculentum Moench (buckwheat) has flavonoids, phenolic acids, tannins and high content of lysine, and the highest levels of these compounds are found in the seeds. Tannins exert direct anthelmintic action in reducing the the fertility of female nematodes, and indirect by increasing the immune response to protect the ingested protein of rumen degradation. The objective of this study was to evaluate the anthelmintic potential of the hydroalcoholic extract of F. esculentum Moench seeds (ETM) in the control of gastrointestinal nematodes of sheep in vitro and the antioxidant activity of ETM. Faeces from sheep presenting at least 2,000 eggs per gram of faeces were used. Hatchability and larvae migration tests were performed to evaluate the treatments ETM at concentrations of 0.625; 1.25; 2.5 and 5 mg mL-1 , negative and positive controls and DMSO control (0.75% + distilled water). The treatment means were compared by Tukey test at 5% of probability. Subsequently, the total content of polyphenols, flavonoids, tannins and antioxidant activity of ETM were determined. For the conditions evaluated in this study, it was possible to verify anthelmintic activity of ETM in both tests in vitro. The ETM inhibited 19.66% of hatching in concentration 1.25 mg mL-1 and 17.66% of larvae migration in the concentration of 5 mg mL-1. The anthelmintic activity may be due to condensed tannin content found (288.89 mg equivalent tannic acid per gram of extract). Antioxidant activity was observed at all concentrations, reaching 38.71% at 3 mg mL-1 with IC 50% = 3.83 mg mL-1 and 468.12 µM equivalent Trolox per gram of extract. At the same concentration to flavonoids and total polyphenols was observed respectively 31 mg equivalent rutin and 54.33 mg equivalent gallic acid per gram of extract. In addition to the direct effect of ETM on trichostrongylids of sheep, future research is also justified by the possibility of an indirect effect due to immune stimuli that protein diet provides on fostering in combating worms. It was concluded that it was possible to verify anthelmintic and antioxidant activity, demonstrating the potential of ETM in parasitological control of sheep.

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Section: Resultsmentioning

confidence: 97%

Anthelmintic and antioxidant potential of Fagopyrum esculentum Moench in vitro

Gonçalves¹,

Debiage²,

Yoshihara³

et al. 2016

Afr. J. Agric. Res.

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“…The cuticle of H. contortus was severely deformed following treatment with Acacia mearnsii extract (Yoshihara et al, 2015). Millettia pachycarpa root bark extract also caused damaging effects on the cuticle of A. galli, including shrinkage and collapse of the lips (Lalchhandama, 2019).…”

Section: Resultsmentioning

confidence: 99%

Nematocidal effects of tobacco infusion (tuibur) against intestinal helminth parasites of chicken

Lalruatfela¹,

Lalthanpuii²,

Lalnunfela³

et al. 2020

JEB

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To study the effect of tobacco infusion, locally known as tuibur in Mizoram, as an antiparasitic agent. A traditionally prepared tobacco infusion was tested for its possible effects on the nematode parasite, Ascaridia galli. The tobacco infusion was prepared in an exponential increase of concentrations, viz. 12.5, 25 and 50% from the original solution. Live and sentient A. galli were collected from the intestines of freshly slaughtered chicken, Gallus gallus domesticus. They were maintained in culture plates that contained phosphatebuffered saline (PBS) in a biological incubator maintained at 37±1ºC. Both tobacco infusion and standard drug showed a dose-dependent efficacy against the nematodes. Scanning electron microscopy of tobacco infusion-treated nematodes showed extensive structural damage. The sensory amphid w a s r u p t u r e d , a n d t h e surrounding lips collapsed. The cuticle was distorted and shrunk all over the body as patchy rows of longitudinal corrugations. Our data shows evidence that tobacco infusion has anthelmintic activity on parasitic nematodes and may serve as a promising candidate for pharmaceutical development. A n t h e l m i n t i c , Ascaridia galli, Nematode, Tobacco infusion, Tuibur Nematocidal effects of tobacco infusion (tuibur) against intestinal helminth parasites of chicken

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“…25 Natural cysteine proteinases also exert anthelmintic activity by primarily attacking the cuticle in different roundworms. 26,27 Extract of Acacia mearnsii was shown to cause shrinkage and severe destruction on the cuticle of H. contortus 28 . We have also shown that a related plant Acacia oxyphylla extract primarily acted through the cuticle to cause tissue damages in the internal musculature in A. galli.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Anthelmintic Activity of Millettia pachycarpa Root Bark Extract on an Intestinal Roundworm, Ascaridia galli

Lalchhandama¹

2019

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Background: Millettia pachycarpa Benth. is member of the family Fabaceae and is a wellknown traditional medicinal plant used for different health conditions. Its roots and seeds are rich in bioactive compounds such as chalcones, isoflavones and rotenoids, which are attributed to the anticancer, antiparasitic and insecticidal properties of the plant. In Mizo traditional medicine, the root bark is also used for parasitic infections. Objective: The study was designed to understand the anthelmintic efficacy and effects of M. pachycarpa on an intestinal roundworm, Ascaridia galli. Materials and Methods: The methanolic extract of the root bark was prepared and survival test was performed against A. galli. The structural effects were studied using scanning electron microscopy and histological preparations. Results: The roundworm showed extensive structural changes and damages. The cuticle showed extensive shrinkage and cracks. Lacerations and scars distorted the fine transverse rings. The mouthparts collapsed with shrunken lips and degenerated denticles. Internal tissues such as musculature and reproductive organs were also impaired. Conclusion: The findings indicate that the plant extract is effective against parasitic roundworms.

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Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract

Cited by 16 publications

References 15 publications

Anthelmintic and antioxidant potential of Fagopyrum esculentum Moench in vitro

Anthelmintic and antioxidant potential of Fagopyrum esculentum Moench in vitro

Nematocidal effects of tobacco infusion (tuibur) against intestinal helminth parasites of chicken

Anthelmintic Activity of Millettia pachycarpa Root Bark Extract on an Intestinal Roundworm, Ascaridia galli

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