Ultrastructural changes in the oviductal mucosa throughout the sexual cycle inBufo arenarum

Winik, Beatriz C.; Alcaide, María F.; Crespo, Claudia A.; Medina, Marcela F.; Ramos, Inés; Fernández, Silvia N.

doi:10.1002/(sici)1097-4687(199901)239:1<61::aid-jmor4>3.0.co;2-n

Cited by 15 publications

(22 citation statements)

References 29 publications

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“…In this way a close correlation can be established between the ultrastructural variability of the secretory granules present in the cells in charge of the secretion of the jelly components (Winik et al, 1999), the chemical variability of the products stored in these granules (Alcaide & Cruz López, 2002) and the variability of the components present in the secretion.…”

Section: Discussionmentioning

confidence: 83%

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Analysis ofBufo arenarumoviductal secretion during the sexual cycle

Crespo

Ramos²,

Medina³

et al. 2009

Zygote

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SummaryBufo arenarum oocytes are oviposited surrounded by jelly coats, one component of the extracellular matrix required for fertilization. The secretion, released to the oviductal lumen, was analysed by SDS-PAGE. The coomassie blue staining evidenced an electrophoretic pattern with molecules ranging between 300 and 19 kDa that showed variations in their secretion profiles during the sexual cycle. In the preovulatory period the densitometric analysis showed the presence of nine peaks with marked predominance of the 74 kDa molecule. Once ovulation has occurred, the jelly coats become arranged around the oocytes during their transit throughout the oviductal pars convoluta (PC), revealing the addition of three proteins only observed during this period, which suggests a differential secretion. Some of these proteins could not diffuse under any extraction treatment, indicating for them a structural or in situ function. Proteins of low molecular mass diffused totally while others showed a partial diffusing capacity. After ovulation a marked decrease in the relative amount of all the proteins released to the lumen, especially the 74 kDa protein, could be detected. During this period, unlike the other stages of the sexual cycle, a differential secretion pattern was observed along the PC. The histochemical analysis performed during the ovulatory period showed the presence of glycoconjugates including both acidic and neutral groups. The present results are in agreement with previous ultrastructural and histochemical studies that describe the role of Bufo arenarum jelly coats in fertilization.

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Section: Discussionmentioning

confidence: 83%

“…Histological (Moreno, 1972) and ultrastructural studies (Winik et al, 1999) demonstrate that, in Bufo arenarum, the oviduct is divided into three main zones (Fig. 1A, B).…”

Section: Introductionmentioning

confidence: 99%

Analysis ofBufo arenarumoviductal secretion during the sexual cycle

Crespo

Ramos²,

Medina³

et al. 2009

Zygote

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“…In this cycle two periods can be identified, a breeding period and a post-reproductive one. During the breeding period, in the spring to summer months, both ovary and oviduct reach their maximum development and functionality (Hedrick & Nishihara, 1991; Winik et al , 1999; Fernández & Ramos, 2003). The post-reproductive period, during the fall to winter months, is characterized by the recovery of the organs involved in reproduction (Fernández & Ramos, 2003).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of the oviductal mucosa of Leptodactylus chaquensis. Analysis of the preovulatory and postovulatory periods

Medina

Ramos

Crespo

et al. 2014

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In the present study we analysed the ultrastructural characteristics of the oviductal mucosa of Leptodactylus chaquensis during the preovulatory period and immediately after ovulation. Epithelial secretory cells, ciliated cells, basal cells and glandular secretory cells are described. During the preovulatory period, the oviduct exhibits its maximum degree of development at both the epithelial and the glandular levels, with numerous secretory cells that contain a large number of secretory granules whose contents are released into the oviductal lumen by apocrine and exocytotic secretory processes. The secretory cells present throughout the oviduct display considerable variability in the characteristics of their secretory granules, which show different shapes, sizes, organization of the material contained and electron density. The different cell types are distributed following a characteristic pattern for each oviductal zone, thus creating an ultrastructural mosaic along the oviduct. During the postovulatory period, the number of secretory cells decreases and the remaining ones exhibit a marked reduction in secretory granules. Ciliated cells show a typical ultrastructural organization that is not modified throughout the reproductive cycle. Basal cells, located at the basal region of the epithelium, are characterized by their heterochromatic nuclei and electron-lucent cytoplasm, while glandular secretory cells exhibit oval, round or polyhedric granules, most of them with a prominent core. Our results, which indicate a high heterogeneity of secretory cell contents, allow us to suggest differential synthesis and secretion of specific products in each oviductal zone.

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“…In the toad Rhinella arenarum , as in other anuran amphibians, the oviducts are structurally and functionally divided into three main zones: the pars recta (PR), the pars convoluta (PC) and the ovisac or uterus (Moreno, ; Fernández et al, ; Winik et al, ; Medina et al, ). In these vertebrates, two types of oviductal secretion have been identified, each of them produced by a different portion of the duct and with a different biological role in fertilization.…”

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confidence: 99%

“…The second secretion is the jelly, a highly viscous material that is deposited on the oocytes whereas they are being transported through the PC. Histological (Crespo et al, ) and ultrastructural (Winik et al, ) studies demonstrated that in R. arenarum four jelly coats are sequentially formed by components provided by both the ESC and the glandular secretory cells (GSC). These cells are located along the different portions of the oviductal PC, termed intermediate proximal zone (IPZ), pars preconvoluta (PPC) and pars convoluta itself (pc), all of which exhibit conspicuous changes in their structural organization (Winik et al, ; Crespo et al, ) and secretory activity (Medina et al, ; Crespo et al, ) throughout the reproductive cycle.…”

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confidence: 99%

Homeostasis and secretion of calcium in the oviductal mucosa of toad Rhinella arenarum

et al. 2014

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The presence of a calcium pump, calbindin D-28KD, and calmodulin in the secretory cells (SC) of the oviductal pars convoluta (PC) of Rhinella arenarum was established for the first time in amphibians using immunohistochemical techniques. Marked variations were observed in the localization and degree of expression of these proteins according to the duct segment and the period of the sexual cycle analyzed. During the preovulatory and ovulatory periods the calcium pump colocalized with calbindin D-28KD can be seen mainly in the apical border of the SC, which are located in the first zones of PC and synthesize and secrete the components of the inner jelly coat layers. These envelopes, which surround the oocytes, contain the molecules indispensable for fertilization, probably inducing the sperm acrosome reaction (AR). Our results suggest that calmodulin, colocalized with the calcium pump at the SC cytoplasmic level, would be involved in the active transport of the cation inside the secretory granules, maintaining adequate levels of intracellular Ca(2+) . During the postreproductive period, a calcium pump colocalized with calbindin D-28KD appears for the first time in the cycle in the basal zones of the SC. This system may be related to the replenishing of intracellular Ca(2+) stores. In contrast, in R. arenarum the Ca(2+) present in the jelly coats that surround the oocytes participates in the AR during fertilization, suggesting that this secretion system of the cation provided by the oviductal mucosa is functionally more active during the reproductive period of this species.

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Ultrastructural changes in the oviductal mucosa throughout the sexual cycle inBufo arenarum

Cited by 15 publications

References 29 publications

Analysis ofBufo arenarumoviductal secretion during the sexual cycle

Analysis ofBufo arenarumoviductal secretion during the sexual cycle

Ultrastructure of the oviductal mucosa of Leptodactylus chaquensis. Analysis of the preovulatory and postovulatory periods

Homeostasis and secretion of calcium in the oviductal mucosa of toad Rhinella arenarum

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