Ultrastructural changes of cornea after ethanol ingestion in Otsuka Long-Evans Tokushima fatty (OLETF) and Long-Evans Tokushima Otsuka (LETO) rats

Kim, Eun Chul; Kim, Daijin; Lee, Seong Su; Kim, Man Soo

doi:10.1007/s00417-010-1432-8

Cited by 6 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Abnormal structure and distribution of the hemidesmosomes of the corneal epithelium are related to reduced epithelial adhesion 12 , 13 and delayed corneal epithelial wound closure. 24 We found that the expression of collagen IV in the corneal epithelial basement membrane was reduced in Col4a3 −/− mice. The ultrastructure of the corneal epithelial basement membrane revealed that the hemidesmosome structure was abnormal in the Col4a3 −/− mice.…”

Section: Discussionmentioning

confidence: 68%

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome

Wang,

Zhu,

Zhao

et al. 2024

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

Purpose The purpose of this study was to investigate the ocular morphological characteristics of Col4a3 −/− mice as a model of Alport syndrome (AS) and the potential pathogenesis. Methods The expression of collagen IV at 8, 12, and 21 weeks of age was evaluated by immunohistochemistry in wild-type (WT) and Col4a3 −/− mice. Hematoxylin and eosin (H&E) staining and thickness measurements were performed to assess the thickness of anterior lens capsule and retina. Ultrastructure analysis of corneal epithelial basement membrane, anterior lens capsule, internal limiting membrane (ILM), and retinal pigment epithelium (RPE) basement membrane was performed using transmission electron microscopy. Finally, Müller cell activation was evaluated by glial fibrillary acidic protein (GFAP) expression. Results Collagen IV was downregulated in the corneal epithelial basement membrane and ILM of Col4a3 −/− mice. The hemidesmosomes of Col4a3 −/− mice corneal epithelium became flat and less electron-dense than those of the WT group. Compared with those of the WT mice, the anterior lens capsules of Col4a3 −/− mice were thinner. Abnormal structure was detected at the ILM Col4a3 −/− mice, and the basal folds of the RPE basement membrane in Col4a3 −/− mice were thicker and shorter. The retinas of Col4a3 −/− mice were thinner than those of WT mice, especially within 1000 µm away from the optic nerve. GFAP expression enhanced in each age group of Col4a3 −/− mice. Conclusions Our results suggested that Col4a3 −/− mice exhibit ocular anomalies similar to patients with AS. Additionally, Müller cells may be involved in AS retinal anomalies. Translational Relevance This animal model could provide an opportunity to understand the underlying mechanisms of AS ocular disorders and to investigate potential new treatments.

show abstract

Section: Discussionmentioning

confidence: 68%

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome

Wang,

Zhu,

Zhao

et al. 2024

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

show abstract

“…Expression of Cytochrome p450 2E1 (CYP2E1) mRNA, CYP2E1 protein activity, and production of reactive oxygen species were determined to be induced by ethanol in a concentration-dependent manner in retinal pigment epithelium [20]. Moreover, chronic alcohol consumption was also associated with the ultra-structural alterations on cornea [21].…”

Section: Discussionmentioning

confidence: 99%

The effects of thiamine pyrophosphate on ethanol induced optic nerve damage

Uçak

Karakurt

Tasli

et al. 2019

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

Background We aimed to determine the protective effects of thiamine pyrophosphate on ethanol induced optic neuropathy in an experimental model. Methods The rats were assigned into 4 groups, with 6 rats in each group as follows: healthy controls (HC group), only ethanol administered group (EtOH group), ethanol + thiamine pyrophosphate (20 mg/kg) administered group (TEt-20 group), and only thiamine pyrophosphate (20 mg/kg) (TPG group) administered group. To the rats in TEt-20 and TPG groups, 20 mg/kg thiamine pyrophosphate was administered via intraperitoneal route. To the rats in HC and EtOH groups, the same volume (0.5 ml) of distilled water as solvent was applied in the same manner. To the rats in TEt-20 and EtOH groups, one hour after application of thiamine pyrophosphate or distilled water, 32% ethanol with a dose of 5 g/kg was administered via oral gavage. This procedure was repeated once a day for 6 weeks. From the blood samples and tissues obtained from the rats, Malondialdehyde (MDA), reduced glutathione (GSH), interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) levels were studied. Histopathological evaluations were performed to the optic nerve tissue. Results Serum and tissue IL-1β, TNF-α and MDA levels were the highest in EtOH group which were significantly lower in thiamine pyrophosphate administered group (TEt-20 group) (p: 0.001). Serum and tissue reduced GSH levels were the lowest in EtOH group which were also significantly higher in TEt-20 group (p:0.001). In histopathological evaluations, in EtOH group there was obvious destruction and edema with hemorrhage and dilated blood vessels which were not present in any other groups. Conclusions There was an apparent destruction in ethanol administered group in histopathological analyses with an augmented level of oxidative stress markers and all those alterations were prevented with concomitant thiamine pyrophosphate administration. These protective effects of thiamine pyrophosphate are extremely important in chronic ethanol consumption. Clinical studies are warranted to define the exact role of thiamine pyrophosphate in prevention of ethanol induced optic neuropathy.

show abstract

“…Diminished expression of the tight junction proteins including β-catenin and zonula occludens-1 was also observed, indicating the disruption of cell to cell junctions in diabetic cornea 53. Other animal studies showed detrimental effects of hyperglycemia on the corneal epithelium basement membrane complex and demonstrated findings suggesting compromise of corneal epithelial function, such as increase in corneal thickness, disruption of tight junctions, and loss of basal epithelial cells 19,54. The accumulation of AGE in the corneal epithelium basement membrane complex promotes proapoptotic and antiproliferative pathways, which results in corneal epithelial damage 19.…”

Section: Ocular Surface Abnormalities In Dmmentioning

confidence: 99%

Influence of diabetes mellitus on anterior segment of the eye

Han

Yang

Hyon

2018

CIA

View full text Add to dashboard Cite

Diabetes mellitus (DM) has been emerging as one of the most serious health problems worldwide. Ocular complications of DM are currently one of the major causes of blindness in developed countries, among which diabetic retinopathy is relatively well studied and understood. However, although ocular surface complications of DM are common, diabetic complications of anterior segment of the eye, such as, cornea, conjunctiva, and lacrimal glands, are often overlooked. DM is associated with progressive damage to corneal nerves and epithelial cells, which increases the risk of anterior segment disorders including dry eye disease, corneal erosion, persistent epithelial defects, and even sight-threatening corneal ulcer. In this review, the authors will discuss the association of DM with disorders of anterior segment of the eye. Studies indicating the value of corneal nerve assessment as a sensitive, noninvasive, and repeatable biomarker for diabetic neuropathy will also be introduced. In addition, treatment modalities of anterior segment disorders associated with DM is discussed. The studies introduced in this review suggest that early and periodic screening of the anterior segment of the eye, as well as the retina, is important for the optimal treatment of DM.

show abstract

Ultrastructural changes of cornea after ethanol ingestion in Otsuka Long-Evans Tokushima fatty (OLETF) and Long-Evans Tokushima Otsuka (LETO) rats

Cited by 6 publications

References 31 publications

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome

The effects of thiamine pyrophosphate on ethanol induced optic nerve damage

Influence of diabetes mellitus on anterior segment of the eye

Contact Info

Product

Resources

About

Ultrastructural changes of cornea after ethanol ingestion in Otsuka Long-Evans Tokushima fatty (OLETF) and Long-Evans Tokushima Otsuka (LETO) rats

Cited by 6 publications

References 31 publications

Characterization of Ocular Morphology in Col4a3−/− Mice as a Murine Model for Alport Syndrome

Characterization of Ocular Morphology in Col4a3−/− Mice as a Murine Model for Alport Syndrome

The effects of thiamine pyrophosphate on ethanol induced optic nerve damage

Influence of diabetes mellitus on anterior segment of the eye

Contact Info

Product

Resources

About

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome

Characterization of Ocular Morphology in Col4a3^−/− Mice as a Murine Model for Alport Syndrome