Ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity

Matsubayashi, Makoto; Suzuta, Fumiko; Terayama, Yoshimi; Shimojo, Kengo; Yui, Takeshi; Haritani, Makoto; Shibahara, Tomoyuki

doi:10.1007/s00436-014-3965-y

Cited by 41 publications

(22 citation statements)

References 22 publications

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“…It is noteworthy that Entamoeba suis was not detected. A study of five pigs taken from a population of 148 displaying hemorrhagic colitis symptoms in Japan suggested that E. suis was responsible for the disease (Matsubayashi et al 2014). Conversely, E. suis was detected in 28 specimens taken from pigs in Vietnam and no ill health was reported (Clark et al 2006).…”

Section: Entamoeba In Pigsmentioning

confidence: 99%

Expanding the Entamoeba Universe: New Hosts Yield Novel Ribosomal Lineages

Jacob

Busby

Levy

et al. 2015

J Eukaryotic Microbiology

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24Removing the requirement for cell culture has led to a substantial increase in the number 25 of lineages of Entamoeba recognized as distinct. Surveying the range of potential host 26 species for this parasite genus has barely been started and it is clear that additional 27 sampling of the same host in different locations often identifies additional diversity. In 28 this study, using small subunit ribosomal RNA gene sequencing, we identify four new 29 lineages of Entamoeba, including the first report of Entamoeba from an elephant, and 30 extend the host range of some previously described lineages. Additionally, examination 31 of microbiome data from a number of host animals suggests that substantial Entamoeba 32 diversity remains to be uncovered. 33 34Keywords: Diversity; next generation sequencing; ribosomal RNA; phylogeny; 35 36 37Over the past 25 years, our understanding of diversity in the genus Entamoeba has 38 increased significantly as a result of complementary developments in DNA amplification, 39 purification and sequencing. Traditionally, naming of species in Entamoeba was based on 40 a mixture of host identity and parasite morphology, the latter being rather limited in these 41 amoeboid organisms and the former being of debatable value due to uncertainty over host 42 ranges of the parasites. DNA sequencing allows quantitative measurement of similarity 43 that is not dependent on such characters and although it is not without its own limitations, 44 it has fundamentally changed our approach to studying diversity in organisms such as 45Entamoeba. 46In this time period, the study of Entamoeba has gone from being dependent on 47 stable laboratory cultures of parasites, preferably in the axenic form, to DNA analysis of 48 organisms directly from stool samples in the absence of even microscopic investigation. 49The latter aspect has been problematic as it is not possible to assign new sequences to 50 previously named species where the original description is dependent on morphology. 51For this reason, many new and distinct Entamoeba sequences have been assigned to 52 'ribosomal lineages' rather than species to reflect the absence of morphological 53 information (Stensvold et al. 2011). 54The new approach is dependent on the reliability of DNA purification from stool 55 samples, which are notorious for the presence of enzyme inhibitors, and the specificity of 56 the primers used for PCR amplification. In addition, investigation of Entamoeba in such 57 samples is largely limited to the ribosomal RNA genes due to the complexity of the 58 DNAs extracted from stool, which often contains DNA from multiple other parasites and 59 may include multiple Entamoeba species. The elimination of culture dependency has led 60 to a dramatic expansion in the number of genetically distinct Entamoeba organisms being 61 recognized but also to a greater understanding of sequence variability within species due 62 to the relative ease with which multiple samples can be studied in parallel.

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Section: Entamoeba In Pigsmentioning

confidence: 99%

Expanding the Entamoeba Universe: New Hosts Yield Novel Ribosomal Lineages

Jacob

Busby

Levy

et al. 2015

J Eukaryotic Microbiology

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“…Entamoeba trophozoites were found in the lumen and/or intestinal lesions in 53/206 (25.7%) pigs from 28/53 (52.8%) farms. The trophozoites were morphologically distinguished as E. suis-like (with the cytoplasm containing one nucleus and ingested host red blood cells visible as small dark bodies) (Matsubayashi et al 2014) or E. polecki-like (with the cytoplasm containing one nucleus and some vacuoles) (Matsubayashi et al 2015a(Matsubayashi et al , 2015b(Matsubayashi et al , 2016. Most of the pigs with trophozoites were approximately 60 days old (average: 60.8 days; median: 60.0 days).…”

Section: Clinical and Subclinical Entamoeba Infections And Tissue Dismentioning

confidence: 99%

“…The following primers were used: EhL-EhR multiplex primer sets targeting the SSU rRNA gene of E. histolytica (or EdL-EdR for E. dispar) (Evangelopoulos et al 2000), 764-RD3 and 764-765 for nested PCR detection of E. suis (Clark et al 2006), and Epolec F6-R6 primers to distinguish among E. polecki subtypes (Matsubayashi et al 2015b). DNA from a human isolate of E. histolytica (unpublished) and from E. suis and E. polecki isolates previously reported by Matsubayashi et al (2014Matsubayashi et al ( , 2015aMatsubayashi et al ( , 2015b) was used as a positive control.…”

Section: Molecular Identification Of Entamoeba Sppmentioning

confidence: 99%

“…are nonpathogenic to pigs; however, both E. suis and E. polecki have been found to cause severe enteritis. E. suis is thought to induce hemorrhagic colitis by invading the lamina propria (Matsubayashi et al 2014). E. polecki ST1 and ST3 are associated with proliferative enteritis caused by Li (Matsubayashi et al 2015a(Matsubayashi et al , 2015b(Matsubayashi et al , 2016, although the ST2 and ST4 subtypes, which have been detected in humans and nonhuman primates (Jirků-Pomajbíková et al 2016, Tuda et al 2016, have not been reported in pigs.…”

Section: Introductionmentioning

confidence: 99%

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Retrospective and Histopathological Studies of <i>Entamoeba</i> spp. and Other Pathogens Associated with Diarrhea and Wasting in Pigs in Aichi Prefecture, Japan

Komatsu¹,

Matsubayashi

Murakoshi

et al. 2019

JARQ

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known to be involved in postweaning wasting (Ellis et al. 1998, Järveots et al. 2016). Salmonellosis, particularly that associated with Salmonella Typhimurium and Salmonella Choleraesuis, can occur concurrently with PMWS (Ha et al. 2005). The enteric protozoan parasites Entamoeba spp., especially E. histolytica, E. polecki (Matsubayashi et al. 2015a, 2015b, 2016), and E. suis (Matsubayashi et al. 2014), were recently identified as the causative agent of diarrhea in pigs. E. polecki can be classified into four subtypes (ST1-ST4) by polymerase chain reaction (PCR) and nucleotide sequencing of the small-subunit ribosomal

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“…The few studies about gut eukaryotes conducted in pigs (Sus scrofa domesticus) used a limited sample size and mainly focused on isolated members of the eukaryote communities or parasite identification under pathogenic conditions Matsubayashi et al 2014;Shieban 1971;Summers et al 2019;White et al 2019;Wylezich et al 2019). In swine, Kazachstania spp.…”

Section: Introductionmentioning

confidence: 99%

Gut eukaryotic communities in pigs: diversity, composition and host genetics contribution

Ramayo-Caldas

Prenafeta²,

Zingaretti

et al. 2020

Preprint

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AbstractThis study aims to characterize commensal fungi and protists inhabiting the gut of healthy pigs, and explore the putative host genetic control over diversity and composition of pig gut eukaryotes. Fecal fungi and protists communities from 514 Duroc pigs of two sexes and two different ages were characterized by 18S and ITS ribosomal RNA gene sequencing. The gut mycobiota was dominated by yeasts, with a high prevalence of Kazachstania spp. Regarding protists, representatives of four genera (Blastocystis, Neobalantidium, Tetratrichomonas and Trichomitus) persisted through more than the 80% of the pigs. Heritabilities for the diversity and abundance of gut eukaryotic communities were estimated with the subset of 60 days aged piglets (N=405). Obtained heritabilities ranged from 0.15 to 0.28, indicating a rather limited host-genetic control. A genome wide association study reported genetic variants associated with the fungal α-diversity (SSC6) and with the abundance of Blastocystis spp. (SSC6, SSC17 and SSC18). Annotated candidate genes (IL23R, IL12RB2, PIK3C3, PIK3CD, HNF4A and TNFRSF9) were mainly related to immunity, gut homeostasis and metabolic processes. Our results point towards a minor and taxa specific genetic control over the diversity and composition of the pig gut eukaryotic communities.

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Ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity

Cited by 41 publications

References 22 publications

Expanding the Entamoeba Universe: New Hosts Yield Novel Ribosomal Lineages

Expanding the Entamoeba Universe: New Hosts Yield Novel Ribosomal Lineages

Retrospective and Histopathological Studies of <i>Entamoeba</i> spp. and Other Pathogens Associated with Diarrhea and Wasting in Pigs in Aichi Prefecture, Japan

Gut eukaryotic communities in pigs: diversity, composition and host genetics contribution

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