Ultrastructural features of the egg envelope of silver carp, Hypophthalmichthys molitrix (Osteichthyes, Cyprinidae)

Esmaeili, Hamid Reza; Johal, M. S.

doi:10.1007/s10641-004-2589-y

Cited by 15 publications

(12 citation statements)

References 16 publications

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Section: Fish Egg Structure and Their Adhesive Propertiesmentioning

confidence: 99%

“…Pelagic eggs usually possess a thin follicular epithelium layer and zona radiata, while demersal eggs are covered by a thicker follicular epithelium layer and a comparatively more complex egg envelope (Stehr & Hawkes 1979). The egg envelope protects the egg from mechanical forces such as water currents (Riehl & Patzner 1998;Esmaeili & Johal 2005;Mansour et al 2009a,b), predators (Riehl & Patzner 1998;Rizzo et al 2002) and bacterial and fungal infection (Paxton & Willoughby 2000;Zelazowska 2010). The egg envelope also acts as a diffusion barrier to chemicals and environmental pollutants (Esmaeili & Johal 2005).…”

Section: Fish Egg Structure and Their Adhesive Propertiesmentioning

confidence: 99%

“…) and bacterial and fungal infection (Paxton & Willoughby ; Zelazowska ). The egg envelope also acts as a diffusion barrier to chemicals and environmental pollutants (Esmaeili & Johal ).…”

Section: Fish Egg Structure and Their Adhesive Propertiesmentioning

confidence: 99%

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Egg stickiness in artificial reproduction of sturgeon: an overview

Siddique

Pšenička

Cosson

et al. 2014

Reviews in Aquaculture

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The egg structure of sturgeon is complex, with multiple micropyles and an envelope structure different from that of teleosts and other fish groups. In the sturgeon, an adhesive layer in the follicular epithelium is responsible for the egg adhesiveness. This is a problem for artificial reproduction of many fish species, including sturgeon, when a large number of eggs are incubated in hatchery conditions. Although several techniques for removing adhesiveness have been developed and successfully applied to eggs of common carp, tench, pikeperch and European catfish, de‐sticking methods for sturgeon are limited. Proteolytic enzymes have been successfully applied to common carp, tench and African catfish eggs, leading to a hatching rate over 80% following 2‐min treatment. In sturgeon, blue clay is the most commonly used de‐sticking substance. The chemical reactions determining egg adhesiveness in sturgeon are poorly studied, and optimum methods for removing stickiness remain to be developed.

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Section: Fish Egg Structure and Their Adhesive Propertiesmentioning

confidence: 99%

Section: Fish Egg Structure and Their Adhesive Propertiesmentioning

confidence: 99%

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Egg stickiness in artificial reproduction of sturgeon: an overview

Siddique

Pšenička

Cosson

et al. 2014

Reviews in Aquaculture

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“…Number, shape and size of micropyle and ultrastructure of egg membrane and of the micropyle canal; the number and length of the longest and shortest ridge in the micropyle region beyond the structures of the sperm cell have been used as taxonomic tool in ichthyology (Esmaeili and Johal, 2005;Esmaeili and Gholamifard, 2012).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

Murgas¹,

Paulino²,

Palhares³

et al. 2017

fisheriessciences

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Abstract:This study was designed to identify and measure the gametes of neotropical fish using ultrastructural analysis. Number, shape and size of micropyle and ultrastructure of egg membrane and of the micropyle canal; the number and length of the longest and shortest ridge in the micropyle region beyond the structures of the sperm cell have been used as taxonomic tool in ichthyology . Gametes of six breeding pairs of Piaractus mesopotamicus, Brycon orbignyanus, Salminus brasiliensis and Prochilodus lineatus were collected, fixed and subjected to scanning electron microscopy for the observation and measurement of the structures. The results showed many similarities between the gametes of the studied species. An array of grooves and folds was observed in the micropyle in all species but not in the Prochilodus lineatus oocytes. The micropyle was funnel-shaped in all species. The oocytes showed a great variation in the diameters of major and minor micropyle ostium, evidencing different reproductive strategies. The sperm cells showed simple structures with ovoide heads, a cylindrical midpiece with a single flagellum and no acrosome. This study may contribute to a better understanding of fish reproductive biology, conservation genetics and breeding studies. Eight hours after the second CECP dose, fish were removed from the aquarium and had their urogenital papilla cleaned and dried with a towel. The collection of gametes began after delicate manual massage of the coelomic wall. This procedure was performed in accordance with the Ethical Principles of Animal Experimentation, Protocol 040/2009 of Lavras Federal University-UFLA. Keywords Ultrastructural analysis of gametesPost-fixation methodology was accomplished in accordance to the methodology employed in the Laboratory of Eletronic Microscopy and Ultrastructural Analysis of Lavras Federal University-UFLA. Samples containing 1mL of semen or 30 oocytes of each species were fixed separately in microtubules containing a solution of 2.5% glutaraldehyde and 2.0% paraformaldehyde in 0.05 M sodium cacodylate buffer, pH 7.2, calcium chloride 0.001 M. The samples were maintained for 24 hours at 4°C. After this, samples were immersed in 1% osmium tetroxide for 4 hours at room temperature and subsequent dehydration through in ascending series of acetone (25%, 50%, 75%, 90% and 100%).Before evaluation in the electron scanning microscope, the oocytes and spermatozoa were dehydrated with a CPD030 critical point instrument. The samples were coated with gold in a SCD 050 vacuum evaporator according to protocols designed in Ultrastructural Analysis and Electronic Microscopy Laboratory of Federal University of Lavras, Brazil. Morphometric analysis of the gametesIn the morphometry of the oocytes, was valued the major diameter (Md), minor diameter (md), total volume and the sperm head width ratio and the major diameter of major micropyle ostium (MSH/MO). In the morphometry of the micropyle (Figure 1) in the oocytes surface, was measured the diameters of the major ostium (MO) and minor...

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“…Although there are some similarities in the egg envelope of teleosts and acipenserids, notable differences between the two groups exist in the micropyle structure (Debus et al., ). The number of micropyle in the eggs, as well as their fine‐structure, is used for taxonomic description (Riehl, ; Chen et al., ; Li et al., ) and can be considered general phylogenetic characteristics of fish (Esmaeili and Johal, ).…”

Section: Introductionmentioning

confidence: 99%

A review of the structure of sturgeon egg membranes and of the associated terminology

et al. 2014

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Knowledge of the morphological properties, species specific differences, development, and function of the sturgeon egg envelope is of importance for a better understanding of sturgeon reproduction as well as for improvement of the caviar quality. The structure of the egg envelope is similar among the various species, comprising an external envelope (thecal cells, basal lamina, and follicular epithelium), a five-layered oocyte envelope (adhesive layer, alveolar layer, epilayer, and zona radiata externa and interna) and a layer of oocyte matrix and cortical granules. Egg diameters (1.5-4.9 mm), structure and number of the micropyles (2-52), and the size of the micropylar field distinguish eggs of the various species. The development of the egg envelope within the ovary comprises five stages, with further changes following fertilization with the formation of a fertilization cone. The mechanism of polyspermy block in sturgeon eggs and the role of the micropyle and cortical granules in polyspermy block are also described in this review. This review provides information on the morphology and the developmental stages of the egg envelope that will further the understanding of egg biology of acipenserid species. Standardization of terminology describing the egg envelope would minimize confusion and be helpful for future work on acipenserid eggs. U.S.

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Ultrastructural features of the egg envelope of silver carp, Hypophthalmichthys molitrix (Osteichthyes, Cyprinidae)

Cited by 15 publications

References 16 publications

Egg stickiness in artificial reproduction of sturgeon: an overview

Egg stickiness in artificial reproduction of sturgeon: an overview

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

A review of the structure of sturgeon egg membranes and of the associated terminology

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